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Gene Expression Profiling with DNA Microarrays

Revolutionary Tools to Help Diagnosis, Prognosis, Treatment Guidance, and Drug Discovery

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Biomarkers in Breast Cancer

Part of the book series: Cancer Drug Discovery and Development ((CDD&D))

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Abstract

DNA microarrays are small solid supports on the surface of which DNA probes for thousands of genes have been orderly arrayed. Hybridization of labeled RNA from tissues or tumors allows evaluation of the relative amount of any specific mRNA present in the samples, depicting its gene expression profile. During development and progression of breast cancers, specific genetic programs are activated, which can be assayed on DNA microarrays. Studies performed so far show that the gene expression profile of a tumor defines its biology, its invasive and metastatic potential, and its responsiveness to treatments. Classification of breast cancer by expression profiling appears a very powerful approach, outperforming all commonly used methods of classification. However, much work has to be done before microarray will give robust information for clinical decisions and become routine practice.

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Author information

Authors and Affiliations

  1. Department of Oncological Sciences, Institute for Cancer Research and Treatment (IRCC), University of Turin, Turin, Italy

    Michele De Bortoli PHD

  2. Unit of Gynecological Oncology, Institute for Cancer Research and Treatment (IRCC), University of Turin, Turin, Italy

    Nicoletta Biglia MD

Authors
  1. Michele De Bortoli PHD
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  2. Nicoletta Biglia MD
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Editor information

Editors and Affiliations

  1. Division of Medical Oncology, “San Filippo Neri” Hospital, Rome, Italy

    Giampietro Gasparini MD

  2. Breast Oncology Program, University of Michigan Comprehensive Cancer Center, Ann Arbor, MI

    Daniel F. Hayes MD

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© 2006 Humana Press Inc., Totowa, NJ

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De Bortoli, M., Biglia, N. (2006). Gene Expression Profiling with DNA Microarrays. In: Gasparini, G., Hayes, D.F. (eds) Biomarkers in Breast Cancer. Cancer Drug Discovery and Development. Humana Press. https://doi.org/10.1385/1-59259-915-X:047

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