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The Oncogenomics Handbook pp 53–67Cite as

From FISH to Proteomics

A Molecular Brush to Define Antitumor Drug Action

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Part of the book series: Cancer Drug Discovery and Development ((CDD&D))

Abstract

Technological milestones in genomics have initiated a new approach in the development of novel anticancer drugs to specific genes. However, the heterogeneity of cancer involving multigene complexity calls upon a complementary approach to effectively develop novel anticancer drugs either to specific tumors or with broad range of anti-tumor activity. Among various techniques, fluorescence in situ hybridization (FISH) provides the opportunity to identify mRNA sequences at the subcellular level and has, therefore, become an important tool in gene expression studies. In our drug discovery and development program, we adopt a new hypothesis focusing on the whole cancer cell as a single target. A component of our unique developmental paradigm includes a drug-action profile paradigm defining the drug-specific antiproliferative effects of newly discovered investigational agents, at the molecular level using a genomic-proteomic interface. Such an approach using multicolor fluorescence hybridization on cDNA microarray and two-dimensional gel electrophoresis called, Painting with a Molecular Brush, has been successfully adopted to unravel the mechanism of action of a new anticancer agent, XK469.

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Authors and Affiliations

  1. Drug Discovery and Development Program, Henry Ford Health System, Detroit, MI

    Balanehru Subramanian PhD, Alexander Nakeff PhD & Frederick Valeriote PhD

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  1. Balanehru Subramanian PhD
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  2. Alexander Nakeff PhD
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  3. Frederick Valeriote PhD
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Editors and Affiliations

  1. CuraGen Corporation, New Haven, CT

    William J. LaRochelle PhD  & Richard A. Shimkets PhD  & 

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© 2005 Humana Press Inc., Totowa, NJ

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Subramanian, B., Nakeff, A., Valeriote, F. (2005). From FISH to Proteomics. In: LaRochelle, W.J., Shimkets, R.A. (eds) The Oncogenomics Handbook. Cancer Drug Discovery and Development. Humana Press. https://doi.org/10.1385/1-59259-893-5:53

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  • DOI: https://doi.org/10.1385/1-59259-893-5:53

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-425-8

  • Online ISBN: 978-1-59259-893-9

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