Abstract
This chapter reviews the use of viral gene transfer to disentangle the complexities of neurogenic hypertension. Viral gene manipulation allows lasting and controllable genetic manipulation in selected areas of the brain in different species and strains, including experiments in the spontaneously hypertensive rat, an established model of hypertension. Recent evidence indicates that, in contrast to pharmacological tools that may act on any cellular target within a given area of the brain, viral vectors deliver transgene in a nonuniform manner, and its concentration in different types of cells may vary greatly. This occurs as a result of both transductional tropism of a viral vector system and the transciptional activity of the promoter in different cellular types which are present in any brain nucleus. Properties of adenoviral and lentiviral vectors are compared and contrasted. Application of viral vectors for overexpression of biologically active molecules, expression of dominant negative proteins, pathway tracking, and other experiments to study central mechanisms of cardiovascular control are discussed. In summary, virally mediated gene delivery to the brain is a powerful research tool that can be used to address a wide range of questions related to mechanisms of human essential hypertension.
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Kasparov, S., Teschemacher, A.G., Paton, J.F.R. (2005). Application of Viral Gene Transfer in Studies of Neurogenic Hypertension. In: Rai, M.K., Paton, J.F.R., Kasparov, S., Katovich, M.J. (eds) Cardiovascular Genomics. Contemporary Cardiology. Humana Press. https://doi.org/10.1385/1-59259-883-8:247
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