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Biological Weapons Defense pp 551–579Cite as

Concepts for the Development of Immunodiagnostic Assays for Detection and Diagnosis of Biothreat Agents

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Part of the book series: Infectious Disease ((ID))

Abstract

An integrated approach to agent detection and identification provides the most reliable laboratory data and is essential to a complete and accurate disease diagnosis. To achieve more rapid biological agent identification with a high level of confidence, a combination of state-of-the-art antigen and nucleic acid analysis methods is required. The most significant problem associated with the development of an integrated diagnostic system has been the inability of antigen-detection technologies to detect agents with sensitivities approaching those of nucleic acid-detection technologies. These differences in assay sensitivity increase the probability of obtaining disparate results, thus complicating medical decisions. However, advances in immunodiagnostic technologies provide the basis for developing antigen-detection platforms capable of meeting stringent requirements for sensitivity, specificity, assay speed, robustness, and simplicity. Such advances must be exploited to increase the confidence of immunodiagnostic testing and to ensure the success of the integrated approach toward diagnosis of disease caused by biological warfare (BW) agents.

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Author information

Authors and Affiliations

  1. Diagnostic Systems Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD

    George V. Ludwig PhD & Cynthia A. Rossi MS

  2. Naval Medical Research Center, Navy Biological Defense Research Directorate, Silver Spring, MD

    Robert L. Bull PhD

Authors
  1. George V. Ludwig PhD
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  2. Cynthia A. Rossi MS
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  3. Robert L. Bull PhD
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Editor information

Editors and Affiliations

  1. Department of Homeland Security, Science and Technology Directorate, National Biodefense Analysis and Countermeasures Center, Fort Detrick, MD

    Luther E. Lindler PhD  & George W. Korch PhD (Deputy Director) &  (Deputy Director)

  2. Department of Bacterial Diseases, Walter Reed Army Institute of Research, Silver Spring, MD

    Luther E. Lindler PhD

  3. Department of Cell Biology and Biochemistry, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD

    Frank J. Lebeda PhD

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© 2005 Humana Press Inc., Totowa, NJ

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Ludwig, G.V., Rossi, C.A., Bull, R.L. (2005). Concepts for the Development of Immunodiagnostic Assays for Detection and Diagnosis of Biothreat Agents. In: Lindler, L.E., Lebeda, F.J., Korch, G.W. (eds) Biological Weapons Defense. Infectious Disease. Humana Press. https://doi.org/10.1385/1-59259-764-5:551

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  • DOI: https://doi.org/10.1385/1-59259-764-5:551

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-184-4

  • Online ISBN: 978-1-59259-764-2

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