Immunoassay of Steroids

  • M. J. WheelerEmail author
  • G. Barnard


Prior to 1970, few chemical methods existed that were appropriate for the determination of nanomolar and picomolar concentrations of steroid hormones in biological fluids. Nevertheless, by the end of the 1960s, several technologies were being proposed for the measurement of steroids in serum and urine, which included gas–liquid chromatography (Collins et al., 1968), double isotope derivatization (Kliman and Peterson, 1960; Gandy and Peterson, 1968), spectrophotometry and fluorometry (Brown et al., 1968).


Direct Assay Label Antigen Corticosteroid Binding Globulin Free Steroid Extraction Assay 
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  1. Abraham GE (1974) Radioimmunoassay of steroids in biological materials. Acta Endocrinol. 75; 7–42.Google Scholar
  2. Ali H, Ghaffari MA, Vanlier JE (1987) Regioselective a-ring iodination of estradiol diacetates. J. Steroid Biochem. Mol. Biol. 28; 21–23.Google Scholar
  3. Aslam M, Dent AH (eds) (1998) Bioconjugation: Protein Coupling Methods for the Biomedical Sciences. Macmillan, London.Google Scholar
  4. Babson AL (2005) United reagent random-access analysers: Immulite® and Immulite 1000. In The Immunoassay Handbook (ed) Wild D. Elsevier, Oxford, pp. 370–373.Google Scholar
  5. Bai C, Schmidt A, Freedman LP (2003) Steroid hormone receptors and drug discovery: therapeutic opportunities and assay designs. Assay Drug Dev. Technol. 1; 843–852.Google Scholar
  6. Bangham D (1988) Reference preparations and matrix effects. In Complementary Immunoassays (ed) Collins WP. Wiley, Chichester, pp. 13–25.Google Scholar
  7. Barnard GJ (1988) The development of fluorescence immunoassays. In Non-radiometric Assay: Technology and Application in Polypeptide and Steroid Hormone Detection (eds) Albertson B, Haseltine F Progress in Clinical and Biological Research, Vol 285. Alan Liss, New York, pp. 153–180.Google Scholar
  8. Barnard G, Kohen F (1990) Idiometric assay: a non-competitive immunoassay for small molecules typified by the measurement of serum estradiol. Clin. Chem. 36; 1945–1950.Google Scholar
  9. Barnard G, Kohen F (1998) Monitoring ovarian function by the simultaneous time-resolved fluorescence immunoassay of two urinary steroid metabolites. Clin. Chem. 44; 1520–1528.Google Scholar
  10. Barnard GJ, Williams JL, Shah HP, et al. (1988) Time-resolved fluoroimmunoassay. In Complementary Immunoassays (ed) Collins WP. Wiley, Chichester, pp. 149–167.Google Scholar
  11. Blockz P, Martin M (2005) Laboratory quality assurance. In The Immunoassay Handbook (ed) Wild D. Elsevier, Oxford, pp 456–471.Google Scholar
  12. Boots LR, Potter S, Potter D, Azziz R (1998) Measurement of total serum testosterone levels using commercially available kits: high degree of between-kit variability. Fertil. Steril. 69; 286–292.Google Scholar
  13. Breuer H, Hamel D, Kruskemper HL (eds) (1976) Methods in Hormone Analysis. Wiley, New York, pp. 1–520.Google Scholar
  14. Brown JB, MacLeod SC, Macnaughtan C, et al. (1968) A rapid method for estimating estrogens in urine using a semi-automatic extractor. J. Endocrinol. 42; 5–15.Google Scholar
  15. Bush VJ, Janu MR, Bathur F, et al. (2001) Comparison of BD vacutainer SSTTM Plus Tubes with BD SSTTM II Plus Tubes for common analytes. Clin. Chim. Acta. 306; 139–143.Google Scholar
  16. Cameron EHD, Scarisbrook JJ, Morris SE, et al. (1974) Some aspects of the use of 125I-labelled ligands for steroid radioimmunoassay. J. Steroid Biochem. 5; 749–756.Google Scholar
  17. Cawood ML, Field HP, Ford CG, et al. (2005) Testosterone measurement by isotope-dilution liquid chromatography-tandem mass spectrometry: validation of a method for routine clinical practice. Clin. Chem. 51; 1472–1479.Google Scholar
  18. Collins WP, Sisterson JM, Koullapis EN, et al. (1968) The evaluation of a gas-liquid chromatographic method for the determination of plasma testosterone using nickel-63 electron capture detection. J. Chromatogr. 37; 33–45.Google Scholar
  19. Cook B, Beastall GH (1987) Measurement of steroid hormone concentrations in blood, urine and tissues. In Steroid Hormones: A Practical Approach (eds) Green B, Leake RE. IRL Press, Oxford, pp. 1–65.Google Scholar
  20. Cook NJ, Read GF, Walker RF, et al. (1992) Salivary cortisol and testosterone as markers of stress in normal subjects in abnormal situations. In Assessment of Hormones and Drugs in Biobehavioural Research (eds) Kirschbaum C, Read GF, Hellhammer D. Hofgrefe & Huber, Seattle, WA.Google Scholar
  21. Corrie JET (1983) [125] Iodinated tracers for steroid radioimmunoassay: the problem of bridge recognition. In Immunoassays for Clinical Chemistry (eds) Hunter WM, Corrie JET. Churchill Livingstone, Edinburgh, pp. 353–357.Google Scholar
  22. Corrie JET, Hunter WM, Macpherson J (1981) A strategy for radioimmunoassay of plasma progester-one with use of a homologous-site 125I-labelled radioligand. Clin. Chem. 27; 594–599.Google Scholar
  23. De Boever J, Kohen F, Usanachitt C, et al. (1986) Direct chemiluminescence immunoassay for estradiol in serum. Clin. Chem. 32; 1985–1990.Google Scholar
  24. Diver MJ (1987) Plasma estradiol concentrations in neonates. Clin. Chem. 33; 1934.Google Scholar
  25. Ekins R (2005) Ambient analyte assay. In The Immunoassay Handbook (ed) Wild D. Elsevier, Oxford, pp 48–62.Google Scholar
  26. Ekins RP (1960) The estimation of thyroxine in human plasma by an electrophoretic technique. Clin. Chim. Acta. 5; 452–459.Google Scholar
  27. Ekins RP (1983a) The precision profile: its use in assay design, assessment and quality control. In Immunoassays for Clinical Chemistry (eds) Hunter WM, Corrie JET. Churchill Livingstone, Edinburgh, pp. 76–104.Google Scholar
  28. Ekins RP (1983b) The direct immunoassay of free (non-protein bound) hormones in body fluids. In Immunoassays for Clinical Chemistry (eds) Hunter WM, Corrie JET. Churchill Livingstone, Edinburgh, pp. 319–337.Google Scholar
  29. Ekins RP (1985) Current concepts and future developments. In Complementary Immunoassays (ed) Collins WP. Wiley, Chichester, pp. 219–237.Google Scholar
  30. Ekins RP (1992) The free hormone hypothesis and measurement of free hormones. Clin. Chem. 38; 1289–1293.Google Scholar
  31. Erlanger BF (1981) The preparation of antigenic hapten-carrier conjugates: a survey. In Methods in Enzymology, Vol 70 (eds) Van Vunakis H, Langone JJ. Academic, New York, pp. 85–104.Google Scholar
  32. Ewis AA, Zhelev Z, Bakalova R, et al. (2005) A history of microarrays in biomedicine. Expert Rev. Mol. Diagn. 5; 315–328.Google Scholar
  33. Ferry JD, Collins S, Sykes E (1999) Effect of serum volume and time of exposure to gel barrier tubes on results for progesterone by Roche Diagnostics Elecsys 2010. Clin. Chem. 45; 1574–1575.Google Scholar
  34. Fraser CG (1992) Biological variation in clinical chemistry - an update - collated data 1988–1991. Arch. Pathol. Lab. Med. 116; 916–923.Google Scholar
  35. Gandy HM, Peterson RE (1968) Measurement of testosterone and 17-ketosteroids in plasma by double isotope dilution derivative technique. J. Clin. Endocrinol. Metab. 28; 949–977.Google Scholar
  36. Gillis EH, Gosling JP, Sreenam JM, et al. (2002) Development and validation of a biosensor-based immunoassay for progesterone in bovine milk. J. Immunol. Meth. 267; 131–138.Google Scholar
  37. Goding JW (1996) Monoclonal antibodies: principles and practice - production and application of monoclonal antibodies in cell biology. In Biochemistry and Immunology. Academic Press, London.Google Scholar
  38. Granger DA, Cicchetti D, Rogosch FA, et al. (2007) Blood contamination in children’s saliva: prevalence, stability, and impact on the measurement of salivary cortisol, testosterone and dehydroepiandrosterone. Psychoneuroendocrinology. 32; 724–733.Google Scholar
  39. Guo TD, Chan M, Soldin SJ (2004) Steroid profiles using liquid chromatography-tandem mass spectrometry with atmospheric pressure photoionization source. Arch. Pathol. Lab. Med. 128; 469–475.Google Scholar
  40. Guo TD, Taylor RL, Singh RJ, et al. (2006) Simultaneous determination of 12 steroids by isotope dilution liquid chromatography-photo spray ionization tandem mass spectrometry. Clin. Chim. Acta. 372; 76–82.Google Scholar
  41. Hadd AG, Brown JT, Fandruss BF, et al. (2005) Adoption of array technologies into the clinical laboratory. Expert Rev. Mol. Diagn. 5; 409–420.Google Scholar
  42. Harlow E, Lane D (1998) Using Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.Google Scholar
  43. Hay ID, Bayer MF, Kaplan MM, et al. (1991) American Thyroid Association assessment of current free thyroid hormone and thyrotropin measurements and guidelines for future clinical assays. Clin. Chem. 37; 2002–2008.Google Scholar
  44. Heald AH, Butterworth A, Kane JW, et al. (2006) Investigation into possible causes of interference in serum testosterone measurement in women. Ann. Clin. Biochem. 43; 189–195.Google Scholar
  45. Hemmila IA (1991) Applications of Fluorescence in Immunoassays. Wiley, New York, pp. 1–344.Google Scholar
  46. Herold DA, Fitzgerald RL (2003) Immunoassays for testosterone in women: better than a guess? Clin. Chem. 49; 1250–1251.Google Scholar
  47. Hommola J (2003) Present and future of surface plasmon resonance biosensors. Anal. Bioanal. Chem. 377; 528–529.Google Scholar
  48. Ismail AAA, Walker PL, Barth JL, et al. (2002) Wrong biochemistry results: two case reports and observational study in 5310 patients on potentially misleading thyroid-stimulating hormone and gonadotropin immunoassay results. Clin. Chem. 48; 2023–2029.Google Scholar
  49. Jackson TM, Ekins RP (1986) Theoretical limitations on immunoassay sensitivity. J. Immunol. Meth. 87; 13–20.Google Scholar
  50. Kabat EA (1981) Basic principles of antigen-antibody reactions. In Methods in Enzymology, Vol 70 (eds) Van Vunakis H, Langone JJ. Academic Press, New York, pp. 3–49.Google Scholar
  51. Kaiser T, Gudat P, Stock W, et al. (2000) Biotinylated steroid derivatives as ligands for biospecific interaction analysis with monoclonal antibodies using immunosensor devices. Anal. Biochem. 282; 173–185.Google Scholar
  52. Kellie AE, Lichman KV, Samarajeewa P (1975) Chemistry of steroid-protein conjugate formation. In Steroid Immunoassay (eds) Cameron EHD, Hillier SG, Griffiths K. Alpha Omega, Cardiff, pp. 33–46.Google Scholar
  53. Kesner JS, Wright DM, Schrader SM, et al. (1992) Methods of monitoring menstrual function in field studies - efficacy of methods. Reprod. Toxicol. 6; 385–400.Google Scholar
  54. Key TJA, Moore JW (1988) Interference of sex-hormone binding globulin in a no-extraction double antibody radioimmunoassay for oestradiol. Clin. Chem. 34; 1357–1358.Google Scholar
  55. Kliman B, Peterson RE (1960) Double isotope derivative assay of aldosterone in biological extracts. J. Biol. Chem. 235; 1639–1648.Google Scholar
  56. Kohler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of pre-defined specificity. Nature. 256; 495–497.Google Scholar
  57. Korenman SG, Perrin LE, Rao BR, et al. (1970) Plasma estrogen assays utilizing the cytoplasmic estrogen binder of rabbit uterus. Res. Steroids. 4; 287–296.Google Scholar
  58. Kricka LJ (1994) Selected strategies for improving sensitivity and reliability of immunoassays. Clin. Chem. 40; 347–357.Google Scholar
  59. Kumar A, Goel G, Fahrenbach E, et al. (2005) Microarrays: the technology, analysis and application. Eng. Life Sci. 5; 215–222.Google Scholar
  60. Lamph SA, Wheeler MJ, Halloran SP (2003a) Dynex Technologies DSX immunoassay analyser. Medical and Healthcare Products Regulatory Agency Evaluation Report: MHRA 0317, Centre for Evidence-Based Purchasing, London.Google Scholar
  61. Lamph SA, Wheeler MJ, Halloran SP (2003b) Eight testosterone assays. Medical and Healthcare Products Regulatory Agency Evaluation Report: MHRA 03127, Centre for Evidence-Based Purchasing, London.Google Scholar
  62. Lamph SA, Wheeler MJ, Halloran SP (2004) Six oestradiol assays. Medical and Healthcare Products Regulatory Agency Evaluation Report: MHRA 04106, centre for evidence-based purchasing, London.Google Scholar
  63. Le Roux CW, Sivakumaran S, Alaghband-Zadeh J, et al. (2002) Free cortisol index as a surrogate marker for free cortisol. Ann. Clin. Biochem. 39; 406–408.Google Scholar
  64. Le Roux CW, Chapman GA, Kong WM, et al. (2003) Free cortisol index is better than free total cortisol in determining hypothalamo-pituitary-adrenal status in patients undergoing surgery. J. Clin. Endocrinol. Metab. 88; 2045–2048.Google Scholar
  65. Leake RE, Habib F (1987) Steroid hormone receptors: assay and characterization. In Steroid Hormones: A Practical Approach (eds) Green B, Leake RE. IRL Press, Oxford, pp. 67–97.Google Scholar
  66. Marks V (2002) False-positive immunoassay results: a multicenter survey of erroneous immunoassay results from assays of 74 analytes in 10 donors from 66 laboratories in seven countries. Clin. Chem. 48; 2008–2016.Google Scholar
  67. Maunsell Z, Wright DJ, Rainbow SJ (2005) Routine isotope-dilution liquid chromatography-tandem mass spectrometry assay for simultaneous measurement of the 25-hydroxy metabolites of vitamins D2 and D3. Clin. Chem. 51; 1683–1690.Google Scholar
  68. Mayes D, Nugent CA (1970) Plasma estradiol determined with a competitive protein binding method. Steroids. 15; 389–403.Google Scholar
  69. McCapra F (1974) Chemiluminescence of organic compounds. Prog. Org. Chem. 8; 231–277.Google Scholar
  70. Medical and Healthcare Products Regulatory Agency (2004) Medical Device Alert for BD vacutainer® SSTTM, and SST IITM AdvanceTM blood collection tubes (glass and plastic). MDA/2004/048.Google Scholar
  71. Middle JG (2007) Dehydroepiandrosterone sulphate interferes in many direct immunoassays for testosterone. Ann. Clin. Biochem. 44; 173–177.Google Scholar
  72. Mikola H, Sundell A-C, Hanninen E (1993) Labeling of estradiol and testosterone alkyloxime derivatives with a europium chelate for time-resolved fluoroimmunoassays. Steroids. 58; 330–334.Google Scholar
  73. Miro F, Coley J, Gani MM, et al. (2004) Comparison between creatinine and pregnanediol adjustments in the retrospective analysis of urinary hormone profiles during the human menstrual cycle. Clin. Chem. Lab. Med. 42; 1043–1050.Google Scholar
  74. Mitchell JS, Wu Y, Cook C, et al. (2006) Estrogen conjugation and antibody binding interactions in surface plasmon resonance biosensing. Steroids. 71; 618–631.Google Scholar
  75. Moal V, Mathieu E, Reynier P, et al. (2007) Low serum testosterone assayed by liquid chromatography-tandem mass spectrometry. Comparison with five immunoassay techniques. Clin. Chim. Acta. 386; 12–19.Google Scholar
  76. Nahoul K, Castanier M, Gervasi G, et al. (1989) Assay of plasma progesterone and testosterone. Comparison of enzyme immunoassays and radioimmunoassays. Ann. Biol. Clin. Sci. Paris. 47; 127–134.Google Scholar
  77. Nars PW, Hunter WM (1973) A method for labelling oestradiol-17 with radioiodine for radioimmunoassay. Endocrinology. 57; xlvii-xlviii.Google Scholar
  78. National Committee for Clinical Laboratory Standards (1992) Evaluation of precision performance of clinical chemistry devices - Second Edition. NCCLS document EP5-T2, NCCLS, Villanova, PA.Google Scholar
  79. Ngo TT, Lenhoff HM (1982) Enzymes as versatile labels and signal amplifiers for monitoring immunochemical reactions. Mol. Cell. Biochem. 44; 3–12.Google Scholar
  80. Nicoloff JT, Spencer CA (1990) The use and misuse of sensitive thyrotropin assays. J. Clin. Endocrinol. Metab. 71; 553–558.Google Scholar
  81. Nieschlag E, Wickings EJ (1975) Review of radioimmunoassay. Z. Klin. Chem. Klin. Bio. 13; 261–271.Google Scholar
  82. Nisbet JA, Jomain PA (1987) Discrepancies in plasma estradiol values obtained with commercial kits. Clin. Chem. 33; 1672.Google Scholar
  83. Novotny M, Wilson DH (2005) Testosterone testing: an immunoassay with improved accuracy in samples from both males and females. Clin. Lab. 29; 26–27.Google Scholar
  84. Petersen PH, Fraser CG, Jorgensen L, et al. (2002) Combination of analytical quality specifications based on biological within- and between-subject variation. Ann. Clin. Biochem. 39; 543–550.Google Scholar
  85. Pratt JJ (1978) Steroid immunoassay in clinical chemistry. Clin. Chem. 24; 1869–1890.Google Scholar
  86. Quinn FA (2005) Achitect® i2000® and i2000®SR analysers. In The Immunoassay Handbook (ed) Wild D. Elsevier, Oxford, pp. 406–411.Google Scholar
  87. Ratcliffe WA (1983) Direct (non-extraction) serum assays for steroids. In Immunoassays for Clinical Chemistry (eds) Hunter WM, Corrie JET. Churchill Livingstone, Edinburgh, pp. 401–409.Google Scholar
  88. Ratcliffe WA, Carter GD, Dowsett M, et al. (1988) Oestradiol assays: applications and guidelines for the provision of a clinical biochemistry service. Ann. Clin. Biochem. 25; 466–483.Google Scholar
  89. Read GF, Walker RF, Wilson DW, et al. (1990) Steroid analysis in saliva for the assessment of endocrine function. Ann. N Y Acad. Sci. 595; 260–274.Google Scholar
  90. Reeves BD, de Souza MLA, Thompson JE, et al. (1970) An improved method for the assay of progesterone by competitive protein binding. Acta Endocrinol. (Kbh. ) 63; 225–241.Google Scholar
  91. Riad Fahmy D, Read GF, Walker RF, et al. (1982) Steroids in saliva for assessing endocrine function. Endocr. Rev. 3; 367–395.Google Scholar
  92. Richardson A, Kim JB, Barnard G, et al. (1985) Chemiluminescence immunoassay of plasma progesterone using progesterone-acridinium ester as the labelled antigen. Clin. Chem. 31; 1664–1668.Google Scholar
  93. Roda A, Girotti S, Piacentini AL, et al. (1986) Development of a sensitive direct luminescent enzyme immunoassay for plasma oestradiol. Ann. Clin. Biochem. 23; 135–145.Google Scholar
  94. Rosner W (2001) An extraordinary inaccurate assay for free testosterone is still with us. J. Clin. Endocrinol. Metab. (Letter) 86; 2903.Google Scholar
  95. Scatchard G (1949) The attraction of protein for small molecules and ions. Ann. N Y Acad. Sci. 51; 660–672.Google Scholar
  96. Schroeder HR, Yeager FM (1978) Chemiluminescence and detection limits of some isoluminol derivatives in various oxidation systems. Anal. Chem. 50; 1114–1120.Google Scholar
  97. Self CH (1985) Antibodies, manufacture and use. International Patent No. WO/85/04422.Google Scholar
  98. Self CH (1989) Determination method, use and components. International Patent No. WO/89/05453.Google Scholar
  99. Self CH, Dessi JL, Winger LA (1994) High-performance assays for small molecules: enhanced sensitivity, rapidity and convenience demonstrated with a noncompetitive immunometric anti-immune complex assay system for digoxin. Clin. Chem. 40; 2035–2041.Google Scholar
  100. Simpson JSA, Campbell AK, Woodhead JS, et al. (1981) Chemiluminescence labels in immunoassay. In Bioluminescence and Chemiluminescence: Basic Chemistry and Analytical Applications (eds) DeLuca M, McElroy WD. Academic Press, New York, pp. 673–679.Google Scholar
  101. Slaats EH, Kennedy JC, Kruijswijk H (1987) Interference of sex-hormone binding globulin in the ‘Coat-a-Count’ testosterone no-extraction radioimmunoassay. Clin. Chem. 33; 300–302.Google Scholar
  102. Stavreus-Evers A, Cekan SZ (2001) Quantitative measurements of steroid receptors and their messenger ribonucleic acids with a special emphasis on polymerase chain reaction. J. Lab. Clin. Med. 137; 383–397.Google Scholar
  103. Sweet F, Patrick TB, Mudd JM (1979) A-ring iodination of estradiol. J. Org. Chem. 44; 2296–2298.Google Scholar
  104. Taieb J, Mathian B, Millot F, et al. (2003) Testosterone measured by 10 immunoassays and isotope-dilution gas chromatography-mass spectrometry in sera from 116 men, women and children. Clin. Chem. 49; 1381–1395.Google Scholar
  105. Tarle M, Padovan R, Spaventi S (1978) Catalytic iodination and direct radiolabelling of dihy-drotestosterone and estradiol-17-diphosphates. J. Labelled Compd. R. 15; 665–671.Google Scholar
  106. Thienpont LM (1998) Standardization of steroid immunoassays - in theory an easy task. Clin. Chem. Lab. Med. 36; 349–352.Google Scholar
  107. Thienpont LM, De Leenheer AP (1998) Efforts by industry toward standardization of serum, estradiol-17|3 measurements. Clin. Chem. 44; 671–674.Google Scholar
  108. Thienpont LM, Van Uytfanghe K, De Leenheer AP (2002) Reference measurement systems in clinical chemistry. Clin. Chim. Acta 323; 73–87.Google Scholar
  109. Thorpe GHG, Kricka LJ, Moseley SB, et al. (1985) Phenols as enhancers of the chemiluminescent horseradish peroxidase-luminol-hydrogen peroxide reaction: application in luminescence monitored enzyme immunoassays. Clin. Chem. 31; 1335–1341.Google Scholar
  110. Titus MA, Gregory CW, Ford OH, et al. (2005) Steroid 5 alpha-reductase isoenzymes I and II in recurrent prostate cancer. Clin. Cancer Res. 11; 4365–4371.Google Scholar
  111. Usuki S, Kondoh K, Kubo T (2000) Plasma endothelin and LH-RH, LH, FSH, prolactin, progesterone, 17 alpha-hydroxyprogesterone, estrone, 17 beta-estradiol, delta(4)-androstenedione, testosterone, active renin, angiotensin-II and ANP levels in blood and LH, estrone and 17 beta-estradiol and pregnanediol levels in urine of normal cycling women. J. Cardiovasc. Pharmacol. 36; S421–S427.Google Scholar
  112. van Weeman BK, Schuurs AHWM (1975) The influence of heterologous combinations of antiserum and enzyme-labelled estrogen on the characteristics of estrogen enzyme-immunoassays. Immunochemistry. 12; 667–670.Google Scholar
  113. Vermeulen A, Verdonck L, Kaufman JM (1999) A critical evaluation of simple methods for the estimation of free testosterone in serum. J. Clin. Endocrinol. Metab. 84; 3666–3672.Google Scholar
  114. Wade S, Haegele AD (1991) Time-integrated measurements of corticosteroids in saliva by oral diffusion sink technology. Clin. Chem. 37; 1166–1172.Google Scholar
  115. Wang C, Catlin DH, Demers LM, et al. (2004) Measurement of total serum testosterone in adult men: comparison of current laboratory methods versus liquid chromatography-tandem mass spectrometry. J. Clin. Endocrinol. Metab. 89; 534–543.Google Scholar
  116. Warner MH, Kane JW, Atkin SL, et al. (2006) Dehydroepiandrosterone sulphate interferes with the Abbott Architect direct immunoassay for testosterone. Ann. Clin. Biochem. 43; 196–199.Google Scholar
  117. Weeks I, McCapra F, Campbell AK, et al. (1982) Immunoassays using chemiluminescent labelled antibodies. In Immunoassays for Clinical Chemistry (eds) Hunter WM, Corrie JET. Churchill Livingstone, Edinburgh, pp. 525–530.Google Scholar
  118. Wheeler MJ (1995) The determination of bio-available testosterone. Ann. Clin. Biochem. 32; 345–357.Google Scholar
  119. Wheeler MJ (2001) Automated immunoassay analysers. Ann. Clin. Biochem. 38; 217–229.Google Scholar
  120. Wheeler MJ, Lowy C (1987) Warning on serum testosterone measurement. Lancet. 29; 514.Google Scholar
  121. Wheeler MJ, Barnes SC (2008) Measurement of testosterone in the diagnosis of hypogonadism in the ageing male. Clin. Endocrinol. 69; 515–525.Google Scholar
  122. Wheeler MJ, Hutchinson JM (eds) (2006) Hormone Assays in Biological Fluids. Humana Press, Totowa, NJ.Google Scholar
  123. Wheeler MJ, D’Souza A, Matadeen J, et al. (1996) Ciba Corning ACS: 180 testosterone assay evaluated. Clin. Chem. 42; 1445–1449.Google Scholar
  124. Wild D (ed) (2005) The Immunoassay Handbook. Elsevier, Oxford, pp 406–411.Google Scholar
  125. Wild D, Kusnezow W (2005) Separation systems. In Immunoassay Handbook (ed) Wild D. Elsevier, Oxford, pp. 177–191.Google Scholar
  126. Wiwanitkit V (2001) Comparison of blood specimens from plain and gel vacuum blood collection tubes. J. Med. Assoc. Thai. 84; 723–726.Google Scholar
  127. Wood P (2009) Salivary steroid assays-research or routine. Ann. Clin. Biochem. 46; 183–196.Google Scholar
  128. Wu Y, Mitchell J, Cook C, et al. (2002) Evaluation of progesterone-ovalbumin conjugates with different length linkers in enzyme-linked immunosorbant assay and surface plasmon resonance-based immunoassay. Steroids. 67; 565–572.Google Scholar
  129. Yalow RS, Berson SA (1960) Immunoassay of endogenous plasma insulin in men. J. Clin. Invest. 39; 1157–1175.Google Scholar
  130. Yaneva M, Mosnier-Pudar H, Dugué M-A, et al. (2004) Midnight salivary cortisol for the initial diagnosis of Cushing’s syndrome of various causes. J. Clin. Endocrinol. Metab. 89; 3345–3351.Google Scholar
  131. Zola H. (1999) Monoclonal Antibodies. Bios Scientific, Oxford.Google Scholar

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© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Department of Chemical PathologyGuy‘s and St. Thomas‘ NHS TrustLondonUK
  2. 2.Centre for Veterinary Science, Department of Veterinary MedicineUniversity of CambridgeCambridgeUK

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