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Immunoassay of Steroids

  • M. J. WheelerEmail author
  • G. Barnard
Chapter

Abstract

Prior to 1970, few chemical methods existed that were appropriate for the determination of nanomolar and picomolar concentrations of steroid hormones in biological fluids. Nevertheless, by the end of the 1960s, several technologies were being proposed for the measurement of steroids in serum and urine, which included gas–liquid chromatography (Collins et al., 1968), double isotope derivatization (Kliman and Peterson, 1960; Gandy and Peterson, 1968), spectrophotometry and fluorometry (Brown et al., 1968).

Keywords

Direct Assay Label Antigen Corticosteroid Binding Globulin Free Steroid Extraction Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Department of Chemical PathologyGuy‘s and St. Thomas‘ NHS TrustLondonUK
  2. 2.Centre for Veterinary Science, Department of Veterinary MedicineUniversity of CambridgeCambridgeUK

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