Abstract
Proteolysis is one of the reasons for poor production of recombinant proteins in Escherichia coli. Important properties of E. coli proteases, which are relevant for the production of recombinant proteins, are reviewed. Furthermore, various strategies to control the proteolysis of the recombinant proteins are presented. These strategies for control of proteolysis can be applied on various stages of the process: design of more stable protein, a modification of the host cell in respect to proteolytic activity, optimisation of cultivation and downstream processing. However, before implementing these measures the proteolysis rate should be measured in order to calculate a potential benefit of reduced proteolysis rate.
This is a preview of subscription content, log in via an institution.
Preview
Unable to display preview. Download preview PDF.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this chapter
Cite this chapter
Rozkov, A., Enfors, SO. Analysis and Control of Proteolysis of Recombinant Proteins in Escherichia coli . In: Physiological Stress Responses in Bioprocesses. Advances in Biochemical Engineering, vol 89. Springer, Berlin, Heidelberg. https://doi.org/10.1007/b95567
Download citation
DOI: https://doi.org/10.1007/b95567
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-20311-7
Online ISBN: 978-3-540-39669-7
eBook Packages: Springer Book Archive