Abstract
In 1995, Wolfenden and Radzicka showed that orotidine 5′-monophosphate decarboxylase (ODCase) was the most proficient enzyme. Since then, the mechanism of catalysis has been widely debated. The recent appearance of crystal structures for ODCase has led not to a definitive picture of catalysis as might be expected, but to even more conjecture concerning the mechanism. In addition, the many theoretical studies on ODCase have caused opinions to diverge, rather than converge, about the mechanism. This review summarizes the mechanistic, crystallographic, and computational evidence for the mechanism of ODCase, and offers a critical evaluation of the various mechanisms based upon this evidence.
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Abbreviations
- ODCase :
-
Orotidine 5′-monophosphate decarboxylase
- OMP :
-
Orotidine 5′-monophosphate
- UMP :
-
Uridine 5′-monophosphate
- BMP :
-
Barbituric acid ribosyl 5′-monophosphate
- 6-azaUMP :
-
6-azauridylate 5′-monophosphate
- XMP :
-
Xanthosine 5′-monophosphate
- CMP :
-
Cytidine 5′-monophosphate
- CPMD :
-
Car-Parrinello molecular dynamics
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Houk, K.N., Tantillo, D.J., Stanton, C., Hu, Y. What Has Theory and Crystallography Revealed About the Mechanism of Catalysis by Orotidine Monophosphate Decarboxylase?. In: Lee, J. (eds) Orotidine Monophosphate Decarboxylase. Topics in Current Chemistry, vol 238. Springer, Berlin, Heidelberg. https://doi.org/10.1007/b94536
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DOI: https://doi.org/10.1007/b94536
Publisher Name: Springer, Berlin, Heidelberg
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