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What Has Theory and Crystallography Revealed About the Mechanism of Catalysis by Orotidine Monophosphate Decarboxylase?

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Book cover Orotidine Monophosphate Decarboxylase

Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 238))

Abstract

In 1995, Wolfenden and Radzicka showed that orotidine 5′-monophosphate decarboxylase (ODCase) was the most proficient enzyme. Since then, the mechanism of catalysis has been widely debated. The recent appearance of crystal structures for ODCase has led not to a definitive picture of catalysis as might be expected, but to even more conjecture concerning the mechanism. In addition, the many theoretical studies on ODCase have caused opinions to diverge, rather than converge, about the mechanism. This review summarizes the mechanistic, crystallographic, and computational evidence for the mechanism of ODCase, and offers a critical evaluation of the various mechanisms based upon this evidence.

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Abbreviations

ODCase :

Orotidine 5′-monophosphate decarboxylase

OMP :

Orotidine 5′-monophosphate

UMP :

Uridine 5′-monophosphate

BMP :

Barbituric acid ribosyl 5′-monophosphate

6-azaUMP :

6-azauridylate 5′-monophosphate

XMP :

Xanthosine 5′-monophosphate

CMP :

Cytidine 5′-monophosphate

CPMD :

Car-Parrinello molecular dynamics

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Author notes

  1. Dean J. Tantillo

    Present address: Department of Chemistry, University of California, One Shields Avenue, Davis, CA , 95616, USA

Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, 90095–1569, USA

    K. N. Houk, Courtney Stanton & Yunfeng Hu

  2. Department of Chemistry and Chemical Biology, Baker Laboratory , Cornell University, Ithaca, NY , 14853–1301, USA

    Dean J. Tantillo

Authors
  1. K. N. Houk
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  2. Dean J. Tantillo
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  3. Courtney Stanton
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  4. Yunfeng Hu
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Corresponding author

Correspondence to K. N. Houk .

Editor information

J.K. Lee

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Houk, K.N., Tantillo, D.J., Stanton, C., Hu, Y. What Has Theory and Crystallography Revealed About the Mechanism of Catalysis by Orotidine Monophosphate Decarboxylase?. In: Lee, J. (eds) Orotidine Monophosphate Decarboxylase. Topics in Current Chemistry, vol 238. Springer, Berlin, Heidelberg. https://doi.org/10.1007/b94536

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  • DOI: https://doi.org/10.1007/b94536

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-20566-1

  • Online ISBN: 978-3-540-40039-4

  • eBook Packages: Springer Book Archive

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