Stress Induced by Recombinant Protein Production in Escherichia coli

Part of the Advances in Biochemical Engineering book series (ABE, volume 89)


Strong production of recombinant proteins interferes with cellular processes in many ways. Drainage of precursors and energy urges the cell to readjust metabolic fluxes and enzyme composition, stress responses are induced, and hence the cellular activity is shifted from growth to reorganisation of biomass. This may result in inhibition of growth or low level of product accumulation. The extent of the bacterial stress response is determined by the specific properties of the recombinant protein, and by the rates of transcription and translation. Taking into account the capacities of the host for protein processing and physiological adaptation, production schemes can be developed that enhance volumetric productivity and sustainability of the process.

Stress response Recombinant protein production Escherichia coli 


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Authors and Affiliations

  1. 1.Institut für BiotechnologieMartin-Luther-Universität Halle-WittenbergHalleGermany
  2. 2.Biochemical Engineering DivisionGBF German Research Centre for BiotechnologyBraunschweigGermany

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