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DNA Immobilization: Silanized Nucleic Acids and Nanoprinting

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Immobilisation of DNA on Chips II

Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 261))

Abstract

Over the last decade, the development of DNA microarray technology has allowed the simultaneous analysis of many thousands of different genes in histological or cytological specimens. Although DNA microarrays can also be fabricated by photolithographic synthesis, the current paper focuses mainly on methods of DNA attachment by spotting on solid surfaces. Chemical modifications on solid surfaces (such as glass) and the DNA molecules have been presented with special focus on the approach of silanized nucleic acids, a method of DNA modification that can rid the need of glass surface modifications, and nanoprinting method, a way of producing high-density DNA arrays by a stamping mechanism. Different glass and DNA modifications have been applied to a different extent in the DNA microarray industry, but it is anticipated that, with the application of the DNA chip shifting gradually from large-scale expression profiling to more focused genotyping or diagnostics, the utilization of these methods may also shift according to the needs.

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Abbreviations

Tris:

tris(hydroxymethyl)aminomethane

PCR:

polymerase chain reaction

ssDNA:

single stranded DNA

DMSO:

dimethylsulfoxide

APS:

ammonium persulfate

TEMED:

N,N,N′,N′-tetramethyl-ethylene-diamine

References

  1. Pollack JR, Perou CM, Alizadeh AA, Eisen MB, Pergamenschikov A, Williams CF, Jeffrey SS, Botstein D, Brown PO (1999) Nature Genet 23:41

    CAS  Google Scholar 

  2. Hacia JG, Fan JB, Ryder O, Jin L, Edgemon K, Ghandour G, Mayer RA, Sun B, Hsie L, Robbins CM, Brody LC, Wang D, Lander ES, Lipshutz R, Fodor SP, Collins FS (1999) Nature Genet 22:164

    CAS  Google Scholar 

  3. Dill K, Stanker LH, Young CR (1999) J Biochem Biophys Methods 41:61

    CAS  Google Scholar 

  4. Drmanac S, Kita D, Labat I, Hauser B, Schmidt C, Burczak JD, Drmanac R (1998) Nature Biotechnol 16:54

    Article  CAS  Google Scholar 

  5. Lipshutz RJ, Fodor SP, Gingeras TR, Lockhart DJ (1999) Nature Genet 21:20

    CAS  Google Scholar 

  6. McGall G, Labadie J, Brock P, Wallraff G, Nguyen T, Hinsberg W (1996) Proc Natl Acad Sci USA 93:13555

    Article  CAS  Google Scholar 

  7. Proudnikov D, Timofeev E, Mirzabekov A (1998) Anal Biochem 259:34

    Article  CAS  Google Scholar 

  8. Chrisey LA, Lee GU, O'Ferrall E (1996) Nucleic Acids Res 24:3031

    Article  CAS  Google Scholar 

  9. Rogers YH, Jiang-Baucom P, Huang ZJ, Bogdanov V, Anderson S, Boyce-Jacino MT (1999) Anal Biochem 266:23

    Article  CAS  Google Scholar 

  10. Cohen G, Deutsch J, Fineberg J, Levine A (1997) Nucleic Acids Res 25:911

    Article  CAS  Google Scholar 

  11. Beier M, Hoheisel JD (1999) Nucleic Acids Res 27:1970

    Article  CAS  Google Scholar 

  12. Guo Z, Guilfoyle RA, Thiel AJ, Wang R, Smith LM (1994) Nucleic Acids Res 22:5456

    Article  CAS  Google Scholar 

  13. Kumar A, Larsson O, Parodi D, Liang Z (2000) Nucleic Acids Res 28:E71

    Article  CAS  Google Scholar 

  14. Schena M, Shalon D, Heller R, Chai A, Brown PO, Davis RW (1996) Proc Natl Acad Sci USA 93:10614

    Article  CAS  Google Scholar 

  15. Lindroos K, Liljedahl U, Raitio M, Syvanen AC (2001) Nucleic Acids Res 29:E69

    Article  CAS  Google Scholar 

  16. Lamture JB, Beattie KL, Burke BL, Eggers MD, Ehrich DJ, Fowler R, Hollis MA, Koskcki BB, Reich RK, Smith SR, Varma RV, Hogan ME (1994) Nucleic Acids Res 22:2121

    Article  CAS  Google Scholar 

  17. Joos B, Koster H, Cone R (1997) Anal Biochem 247:96

    Article  CAS  Google Scholar 

  18. Mirzabekov AD (1994) Trends Biotechnol 12:27

    Article  CAS  Google Scholar 

  19. Hauser NC, Vingron M, Scheideler M, Krems B, Hellmuth K, Entian KD, Hoheisel JD (1998) Yeast 14:1209

    Article  CAS  Google Scholar 

  20. Guschin D, Yershov G, Zaslavsky A, Gemmel A, Shick V, Proudnikov D, Arenkov P, Mirzabekov A (1997) Anal Biochem 250:203

    Article  CAS  Google Scholar 

  21. Yershov G, Barsky V, Belgovskiy A, Kirillov E, Kreindlin E, Ivanov I, Parinov S, Guschin D, Drobishev A, Dubiley S, Mirzabekov A (1996) Proc Natl Acad Sci USA 93:4913

    Article  CAS  Google Scholar 

  22. Dubiley S, Kirillov E, Lysov Yu, Mirzabekov A (1997) Nucleic Acids Res 25:2259

    Article  CAS  Google Scholar 

  23. Afanassiev V, Hanemann V, Wolfl S (2000) Nucleic Acids Res 28:E66

    Article  CAS  Google Scholar 

  24. Southern E, Mir K, Shchepinov M (1999) Nature Genet 21(Suppl):5

    Google Scholar 

  25. Maskos U, Southern E (1992) Nucleic Acids Res 20:1679

    Article  CAS  Google Scholar 

  26. Benters R, Niemeyer CM, Wohrle D (2001) Chembiochem 2:686

    Article  CAS  Google Scholar 

  27. Benters R, Niemeyer CM, Drutschmann D, Blohm D, Wohrle D (2002) Nucleic Acids Res 30:E10

    Article  Google Scholar 

  28. Tomalia DA, Naylor AM, Goddard WA (1990) Angew Chem Int Ed Engl 29:138

    Article  Google Scholar 

  29. Adessi C, Matton G, Ayala G, Turcatti G, Mermod JJ, Mayer P (2000) Nucleic Acids Res 28:E87

    Article  CAS  Google Scholar 

  30. Connolly BA, Rider P (1985) Nucleic Acids Res 13:4485

    Article  CAS  Google Scholar 

  31. Zuckerman R, Corey D, Shultz P (1987) Nucleic Acids Res 15:5305

    Article  Google Scholar 

  32. Sproat BS, Beijer BS, Rider P, Neuner P (1987) Nucleic Acids Res 15:4837

    Article  CAS  Google Scholar 

  33. Li P, Medon PP, Skingle DC, Lanser JA, Symons RH (1987) Nucleic Acids Res 15:5275

    Article  CAS  Google Scholar 

  34. Bischoff R, Coull JM, Regnier FE (1987) Anal Biochem 164:336

    Article  CAS  Google Scholar 

  35. Rehman FN, Audeh M, Abrams ES, Hammond PW, Kenney M, Boles TC (1999) Nucleic Acids Res 27:649

    Article  CAS  Google Scholar 

  36. Mitra RD, Church GM (1999) Nucleic Acids Res 27:E34

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Center for Genomics and Bioinformatics, Karolinska Institutet, 17177, Stockholm, Sweden

    Quan Du, Ola Larsson, Harold Swerdlow & Zicai Liang

  2. Solexa Limited, Chesterford Research Park, Little Chesterford nr. Saffron Walden, CB10 1XL, Essex, UK

    Harold Swerdlow

Authors
  1. Quan Du
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  2. Ola Larsson
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  3. Harold Swerdlow
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  4. Zicai Liang
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Corresponding author

Correspondence to Zicai Liang .

Editor information

Christine Wittmann

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Du, Q., Larsson, O., Swerdlow, H., Liang, Z. DNA Immobilization: Silanized Nucleic Acids and Nanoprinting. In: Wittmann, C. (eds) Immobilisation of DNA on Chips II. Topics in Current Chemistry, vol 261. Springer, Berlin, Heidelberg. https://doi.org/10.1007/b136870

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