Layer-by-layer deposited multilayer assemblies of polyelectrolytes and proteins: from ultrathin films to protein arrays
We have recently introduced a new method of creating ultrathin films of polyelectrolytes based on the electrostatic attraction between opposite charges. Multilayer assemblies are adsorbed in a layer-by-layer fashion from aqueous solutions of the polymers. The total film thickness can easily be adjusted by varying the ionic strength of the solution. Here, we report on the temperature stability and the water content of the multilayer assemblies. Furthermore, we have extended our concept to the incorporation of protein layers into films of synthetic polyelectrolytes. The well established system biotin/streptavidin was used to construct such multilayers, also by biospecific recognition. Adsorption of streptavidin onto previously photostructured precursor films leads to the deposition of the protein on selected areas on the substrate. The films were investigated by small-angle x-ray scattering (SAXS), Fourier Transform Infrared Spectroscopy (FTIR), and by fluorescence microscopy.
Key wordsUltrathin films polyelectrolytes adsorption streptavidin self-assembly photo-structuring biospecific recognition
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