Time-resolved simultaneous small- and wide-angle x-ray diffraction on dipalmitoylphosphatidylcholine by laser temperature-jump

  • G. Rapp
  • M. Rappolt
  • P. Laggner
Conference paper
Part of the Progress in Colloid & Polymer Science book series (PROGCOLLOID, volume 93)


The kinetics of the structural phase transitions of dipalmitoylphosphatidylcholine (DPPC) in excess water have been investigated by simultaneous small- and wide-angle detection in millisecond time-resolved synchrotron x-ray diffraction. The transition from the rippled to liquidcrystalline phase (Pβ(mst) - L a ) has been induced by an approx. 5°C temperature-jump within 2 ms using an erbium glass laser. The structural parameters, i.e., the long-range order of the bilayer stacking and the short-range order of the lateral lipid packing have been detected with 5 ms time-resolution. The experiments show that the intensities of both the small- and the wide-range reflections indicative of the Pβ(mst)-structure decay in parallel and on the same time-scale as the first- and second- order reflections of the lamellar lattice of the L a -phase grow in intensity. For the ppretransition, we have made the important observation that the rearrangement of the hydrocarbon chains is slower than the initial changes in the long-range lamellar lattice.

Key words

Small- and wide-angle x-ray diffraction synchrotron radiation IR-laser T-jump phospholipids phase transitions 


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  1. 1.
    Boulin C, Kempf R, Gabriel A, Koch MHJ (1988) Nucl Instr Methods Phys Res A269:312CrossRefGoogle Scholar
  2. 2.
    Caffrey M (1989) Annu Rev Biophys Chem 18:159–186CrossRefGoogle Scholar
  3. 3.
    Cevc G, Marsh D (1987) Phospholipid Bilayers, Physical Principles and Models Wiley, New YorkGoogle Scholar
  4. 4.
    Cevc G (1991) Chem Phys Lipids, Special Issue on Phospholipid Phase Transitions (Kinnunen P, Laggner P eds) 57:293–307Google Scholar
  5. 5.
    Chapman D, Williams RM, Ladbrooke BD (1967) Chem Phys Lipids 1:445–475CrossRefGoogle Scholar
  6. 6.
    Gabriel A (1977) Rev Sci Instr 48:1303CrossRefGoogle Scholar
  7. 7.
    Janiak M, Small DM, Shipley GG (1976) Biochemistry 15:4575–4580CrossRefGoogle Scholar
  8. 8.
    Gruner SM (1987) Science 238:305–312CrossRefGoogle Scholar
  9. 9.
    Katsaras J, Yang DSC, Epand R (1992) Biophys J 63:1170–1175Google Scholar
  10. 10.
    Koch MHJ, Bordas J (1983) Nucl Instr Methods Phys Res A208:461CrossRefGoogle Scholar
  11. 11.
    Laggner P (1986) New Methods in Absorption, Scattering and Diffraction for Applications in Structural Biology (Chance B, Bartunik HD eds) Academic Press London pp 171–182Google Scholar
  12. 12.
    Laggner P (1988) Top Curr Chem 145:173–202CrossRefGoogle Scholar
  13. 13.
    Laggner P, Kriechbaum M (1991) Chem Phys Lipids, Special Issue on Phospholipid Phase Transitions (Kinnumen P, Laggner P eds) 57:121–145Google Scholar
  14. 14.
    Laggner P, Kriechbaum M, Rapp G (1991) J Appl Cryst 24:836CrossRefGoogle Scholar
  15. 15.
    Laggner P, Kriechbaum M, Hermetter A, Paltauf F, Hendrix J, Rapp G (1989) Progr Colloid Polym Sci 79:33–37Google Scholar
  16. 16.
    Laggner P, Mio H (1992) Nucl Instr Methods Phys Res A323:86–90CrossRefGoogle Scholar
  17. 17.
    Lis LJ, Quinn PJ (1991) J Appl Cryst 24:48–60CrossRefGoogle Scholar
  18. 18.
    Rapp G, Goody RS (1991) J Appl Cryst 24:857CrossRefGoogle Scholar
  19. 19.
    Ruocco MJ, Shipley GG (1982) Biochim Biophys Acta 691:309–320CrossRefGoogle Scholar
  20. 20.
    Small DM (1986) Handbook of Lipid Research, Vol IV: The Physical Chemistry of Lipids, from Alkanes to Phospholipids, Plenum Press, New YorkGoogle Scholar
  21. 21.
    Tardieu A, Luzatti V, Reman FC (1973) J Mol Biol 75:711–733CrossRefGoogle Scholar
  22. 22.
    Tenchov BG, Yao H, Hatta I (1989) Biophys J 56:757Google Scholar
  23. 23.
    Tenchov BG (1991) Chem Phys Lipids, Special Issue on Phospholipid Phase Transitions (Kinnunen P, Laggner P eds) 57:165–177Google Scholar
  24. 24.
    Yao H, Matuoka S, Tenchov BG, Hatta I (1991) Biophys J 59:252Google Scholar

Copyright information

© Dr. Dietrich Steinkopff Verlag GmbH & Co. KG 1993

Authors and Affiliations

  • G. Rapp
    • 1
  • M. Rappolt
    • 1
  • P. Laggner
    • 2
  1. 1.European Molecular Biology Laboratory (EMBL) Hamburg Outstatio nat DESYFRG
  2. 2.Institut für Biophysik und RöntgenstrukturforschungAustrian Academy of SciencesGrazAustria

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