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Time-resolved simultaneous small- and wide-angle x-ray diffraction on dipalmitoylphosphatidylcholine by laser temperature-jump

  • G. Rapp
  • M. Rappolt
  • P. Laggner
Conference paper
Part of the Progress in Colloid & Polymer Science book series (PROGCOLLOID, volume 93)

Abstract

The kinetics of the structural phase transitions of dipalmitoylphosphatidylcholine (DPPC) in excess water have been investigated by simultaneous small- and wide-angle detection in millisecond time-resolved synchrotron x-ray diffraction. The transition from the rippled to liquidcrystalline phase (Pβ(mst) - L a ) has been induced by an approx. 5°C temperature-jump within 2 ms using an erbium glass laser. The structural parameters, i.e., the long-range order of the bilayer stacking and the short-range order of the lateral lipid packing have been detected with 5 ms time-resolution. The experiments show that the intensities of both the small- and the wide-range reflections indicative of the Pβ(mst)-structure decay in parallel and on the same time-scale as the first- and second- order reflections of the lamellar lattice of the L a -phase grow in intensity. For the ppretransition, we have made the important observation that the rearrangement of the hydrocarbon chains is slower than the initial changes in the long-range lamellar lattice.

Key words

Small- and wide-angle x-ray diffraction synchrotron radiation IR-laser T-jump phospholipids phase transitions 

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Copyright information

© Dr. Dietrich Steinkopff Verlag GmbH & Co. KG 1993

Authors and Affiliations

  • G. Rapp
    • 1
  • M. Rappolt
    • 1
  • P. Laggner
    • 2
  1. 1.European Molecular Biology Laboratory (EMBL) Hamburg Outstatio nat DESYFRG
  2. 2.Institut für Biophysik und RöntgenstrukturforschungAustrian Academy of SciencesGrazAustria

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