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A computer program based on the psi function for model-independent analysis of sedimentation equilibrium distributions reflecting macromolecular interactions

  • H. CölfenEmail author
  • D. J. Winzor
Data Analysis
Part of the Progress in Colloid & Polymer Science book series (PROGCOLLOID, volume 107)

Abstract

A computer program, written in BASIC, has been developed for the characterization of either solute self-association or the interaction between macromolecular acceptor and ligand. Simplicity and model independence of the procedure to evaluate the free concentration of smallest macromolecular species are features which are illustrated by analysis of simulated Rayleigh distributions based on α-chymotrypsin dimerization, and of simulated absorbance records of the distribution based on 1:1 interaction between trimethylamine dehydrogenase (acceptor) and electron transferring flavoprotein (ligand).

Key words

Protein self-association heterogeneous association sedimentation equilibrium psi analysis 

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Copyright information

© Dr. Dietrich Steinkopff Verlag GmbH & Co. KG 1997

Authors and Affiliations

  1. 1.Max-Planck-Institute for Colloid and Interface ResearchColloid ChemistryTeltowGermany
  2. 2.Centre for Protein Structure, Function and Engineering Department of BiochemistryUniversity of QueenslandBrisbaneAustralia

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