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Analysis of interaction of the small heat shock protein hsp25 with actin by analytical ultracentrifugation

  • J. Behlke
  • O. Ristau
  • A. Knespel
Conference paper
Part of the Progress in Colloid & Polymer Science book series (PROGCOLLOID, volume 94)

Abstract

By using analytical ultracentrifugation interactions of the small heat shock protein hsp25 and G-actin have been analyzed. Association constants of about 107 reciprocal molar concentration were estimated from the concentration distribution using a computer program based on the nonlinear least-squares methode. In the presence of an excess of actin up to four molecules of this protein were bound to hsp25 particles.

Key words

Analytical ultracentrifugation protein assemblies complex formation association constants nonlinear least-squares techniques 

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Copyright information

© Dr. Dietrich Steinkopff Verlag GmbH & Co. KG 1994

Authors and Affiliations

  • J. Behlke
    • 1
  • O. Ristau
    • 1
  • A. Knespel
    • 1
  1. 1.Max Delbrück Centrum für Molekulare MedizinBerlin

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