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Ultracentrifugation pp 14–19Cite as

Determination of the molar mass of pigment-containing complexes of intrinsic membrane proteins: Problems, solutions and application to the light-harvesting complex B800/820 of Rhodospirillum molischianum

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Part of the book series: Progress in Colloid & Polymer Science ((PROGCOLLOID,volume 94))

Abstract

An adaptation of a procedure for the simultaneous determination of partial specific volumes and molar masses by sedimentation equilibrium experiments (Edelstein SJ, Schachman HK (1967) J Biol Chem 242: 306–311) to intrinsic membrane proteins in detergent solutions is described. The method requires (1) that the protein is available in at least two detergents the densities of which differ as much as possible, and (2) that the effective molar masses of the mixed protein/detergent micelles, M(1 − vρ 0), are determined under conditions of density matching. The method is applied to a pigment-containing membrane protein, the light-harvesting complex B800/820 (LH III) from Rhodospirillum molischianum, in solutions of three detergents. It is shown that the complex is the hexamer of the basic α/β/pigment unit.

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Authors and Affiliations

  1. MPI fÜr Biophysik, Frankfurt am Main, FRG

    D. Schubert, L. Germeroth & H. Michel

  2. Institut für Biophysik der J.W. Goethe-Universität, Theodor-Stern-Kai 7 Haus 74, 60590, Frankfurt/M., FRG

    D. Schubert, C. Tziatzios, J. A. van den Broek & P. Schuck

Authors
  1. D. Schubert
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  2. C. Tziatzios
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  3. J. A. van den Broek
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  4. P. Schuck
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  5. L. Germeroth
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  6. H. Michel
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M. D. Lechner

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© 1994 Dr. Dietrich Steinkopff Verlag GmbH & Co. KG

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Schubert, D., Tziatzios, C., van den Broek, J.A., Schuck, P., Germeroth, L., Michel, H. (1994). Determination of the molar mass of pigment-containing complexes of intrinsic membrane proteins: Problems, solutions and application to the light-harvesting complex B800/820 of Rhodospirillum molischianum. In: Lechner, M.D. (eds) Ultracentrifugation. Progress in Colloid & Polymer Science, vol 94. Steinkopff. https://doi.org/10.1007/BFb0115598

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  • DOI: https://doi.org/10.1007/BFb0115598

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  • Publisher Name: Steinkopff

  • Print ISBN: 978-3-7985-0982-5

  • Online ISBN: 978-3-7985-1675-5

  • eBook Packages: Springer Book Archive

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