Skip to main content
SpringerLink
Log in

Electric field-induced breakdown in lipid membranes

  • Biocolloids
  • Conference paper
  • First Online:
Book cover Trends in Colloid and Interface Science IX

Part of the book series: Progress in Colloid & Polymer Science ((PROGCOLLOID,volume 98))

Abstract

We present a new and fast method to obtain information on macromolecule-lipid interaction. Lipid bilayers were formed without and in presence of macromolecules. Mechanical rupture of the membranes was induced by applying high electric fields. The kinetics of pore formation during irreversible breakdown is sensitive to the binding affinity of macromolecules and the lipid composition of the membrane. We apply this finding to investigate the so-called stealth property of recently introduced PEG-grafted lipids used in liposomal drug carrier systems.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Abbreviations

DPhPC:

diphytanoyl phosphatidylcholine

Stealth lipid, DSPE-PEG:

distearyl phosphatidylethanolamine poly(ethyleneglycol)

EYPC:

egg yolk phosphatidylcholine

HDL:

high density-lipoproteins

References

  1. Borrebueck C, Hagen I (eds.) (1989) Electromanipulation in hybridoma technology. Stockton Press, NY

    Google Scholar 

  2. Zimm BH, Levence SD (1992) Quart Rev Biophys 25:172–04

    Article  Google Scholar 

  3. Orlowski C, Mir LM (1993) Biochim Biophys Acta 1154:51–63

    CAS  Google Scholar 

  4. Wilhelm C, Winterhalter M, Zimmermann U, Benz R (1993) Biophys J 64:121–128

    CAS  Google Scholar 

  5. Klotz K-H, Winterhalter M, Benz R (1993) Biochim Biophys Acta 1147:161–164

    Article  CAS  Google Scholar 

  6. Woodle CM, Lasic DD (1992) Biochim Biophys Acta 1113:171–199

    CAS  Google Scholar 

  7. Patel HM (1992) Critical Rev in Therapeutic Drug Carrier Systems 9:39–90

    CAS  Google Scholar 

  8. Chonn A, Semple SC, Cullis PR (1992) J Biol Chem 267:18759–18765

    CAS  Google Scholar 

  9. Gong EL, Nicholds AV (1979) Lipids 15:86–90

    Article  Google Scholar 

  10. Winterhalter M, Helfrich W (1987) Phys Rev 36A:5874–5876

    Google Scholar 

  11. DeGennes PG (1985) Scaling Concepts in Polymer Physics, Cornell University Press, Ithaca

    Google Scholar 

  12. Redon C, Brochard-Wyart F, Rondelez F (1991) Phys Rev Lett 66:715–718

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biophysical Chemistry, Biozentrum der Universität Basel, Klingenbergstr. 70, 4056, Basel, Switzerland

    M. Winterhalter & K. -H. Klotz

  2. MegaBios Corp., 871-J Industrial Road, 94070, San Carlos, CA, USA

    D. D. Lasic

  3. Lehrstuhl für Biotechnologie, Biozentrum der Universität Würzburg Am Hubland, 97074, Würzburg, FRG

    R. Benz

Authors
  1. M. Winterhalter
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. -H. Klotz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. D. Lasic
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. Benz
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

J. Appell G. Porte

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Dr. Dietrich Steinkopff Verlag GmbH & Co. KG

About this paper

Cite this paper

Winterhalter, M., Klotz, K.H., Lasic, D.D., Benz, R. (1995). Electric field-induced breakdown in lipid membranes. In: Appell, J., Porte, G. (eds) Trends in Colloid and Interface Science IX. Progress in Colloid & Polymer Science, vol 98. Steinkopff. https://doi.org/10.1007/BFb0115244

Download citation

  • DOI: https://doi.org/10.1007/BFb0115244

  • Published:

  • Publisher Name: Steinkopff

  • Print ISBN: 978-3-7985-1031-9

  • Online ISBN: 978-3-7985-1667-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation