Sedimentation velocity analysis of the DNA-protamine interaction
The aim of this work was to investigate the molecular weight of DNA-protamine aggregates in aqueous solution as a function of the [Arg]/[nucleotide] ratio and as a function of the molecular weight distribution of the DNA. In the case of the polydisperse DNA the ultracentrifuge and the UV-absorption measurement show an increasing molecular weight with increasing clupeine content from 2 × 106 of the DNA itself to 18 × 106 of the aggregates. On the other hand, the homodisperse DNA shows the parallel behavior, but the molecular weight of the aggregates is four times higher. The high molecular weight aggregates of both samples have a very broad molecular weight distribution according to the broad sedimentation boundary traces. These high molecular weight aggregates are supposed to form networks of DNA-protamine complexes.
Key wordsUltracentrifuge sedimentation velocity run DNA clupeine protamine aggregation molecular weight distribution
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