Interfacial behaviour of biomacromolecules

  • J. Lyklema
  • W. Norde
Part of the Progress in Colloid & Polymer Science book series (PROGCOLLOID, volume 101)


Biological macromolecules, proteins in particular, belong to the most interesting and challenging surfactants. Many of these molecules are endowed with very special properties, required to carry out specific biological processes. Structure-functioning relationships have been studies for decades by biologists and biochemists, and many applications are now known.

A colloid scientist would interpret biomacromolecules as supramolecular structures that only to a limited extent obey classical colloidal laws. The delicate balance between energetically and entropically driven interactions that determine the special structures and the stability of these molecules is a particularly interesting problem. Offering a surface onto which these molecules can adsorb and monitoring the incurred structural alterations is one approach to obtain more information. Results will be discussed for a number of proteins, including γ-globulins and constituent parts of them. In addition, some new information will be presented about the biopolymers in the surfaces of bacterial cells.

Key words

protein adsorption proteins at interfaces structure immunoglobulins at interfaces bacterial cell walls dielectric dispersions 


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  1. 1.
    Norde W (1986) Adv Colloid Interface Sci 25:267–340CrossRefGoogle Scholar
  2. 2.
    Norde W, Lyklema J (1991) J Biomat Sci, Polymer Edn 2:183–202Google Scholar
  3. 3.
    Haynes CA, Norde W (1994) Colloids Surf B, Biointerfaces 2:517–566CrossRefGoogle Scholar
  4. 4.
    Norde W (1995) Cells and Materials 5:97–112Google Scholar
  5. 5.
    Andrade JD, Hlady V (1986) Adv Polym Sci 79:1–63Google Scholar
  6. 6.
    Sadana A (1992) Chem Revs 92:1799–1818CrossRefGoogle Scholar
  7. 7.
    Lee B, Richards FM (1971) J Mol Biol 55:379–400CrossRefGoogle Scholar
  8. 8.
    Papenhuysen J, Fleer GJ (1984) J Colloid Interface Sci 100:561–570CrossRefGoogle Scholar
  9. 9.
    Norde W, Lyklema J (1978) J Colloid Interface Sci 66:266–276CrossRefGoogle Scholar
  10. 10.
    Lyklema J, Norde W (1995) In: Goodwin JW, Buscal R (eds) Colloidal polymer particles. Academic Press, London, pp 233–243Google Scholar
  11. 11.
    Norde W, Lyklema J (1978) J Colloid Interface Sci 66:295–302CrossRefGoogle Scholar
  12. 12.
    Norde W, Lyklema J (1979) J Colloid Interface Sci 71:350–366CrossRefGoogle Scholar
  13. 13.
    Privalov (1979) Adv Protein Chem 33:167–241CrossRefGoogle Scholar
  14. 14.
    Haynes CA, Norde W (1995) J Colloid Interface Sci 169:313–328CrossRefGoogle Scholar
  15. 15.
    Norde W, Favier JP (1992) Colloids Surf 64:87–93CrossRefGoogle Scholar
  16. 16.
    Master MCL, Pap EHW, van Hoek A, Norde W and Visser AJWG (1995) J Colloid Interface Sci: submittedGoogle Scholar
  17. 17.
    Buijs JAG, (1995) PhD thesis Agricultural University Wageningen NLGoogle Scholar
  18. 18.
    Dijt JC, Cohen Stuart MA and Fleer GJ (1994) Adv Colloid Interface Sci 50:79–101CrossRefGoogle Scholar
  19. 19.
    Dabros T, van de Ven TGM (1983) Colloid Polym Sci 261:694–707CrossRefGoogle Scholar
  20. 20.
    Buijs JAG, White DD and Norde W (1995) J Imm Meth: submittedGoogle Scholar
  21. 21.
    Kyte J, Doolittle RF (1990) J Mol Biol 157:105–132CrossRefGoogle Scholar
  22. 22.
    Elgersma AV, Zsom RLJ, Norde W and Lyklema J (1991) Colloids Surf 54:89–101CrossRefGoogle Scholar
  23. 23.
    Deisenhofer J (1981) Biochemistry 20:2361–2370CrossRefGoogle Scholar
  24. 24.
    Poljak RJ, Amzel LM, Chen BJ, Phizackerly RP and Saul F (1974) Proc Natl Acad Sci USA 71:3440–3444CrossRefGoogle Scholar
  25. 25.
    Van der Wal A, Minor M and Lyklema J (1995) NATO-ASI on Nanoparticles: in pressGoogle Scholar
  26. 26.
    Van der Wal (1996) PhD thesis Agricultural University Wageningen NLGoogle Scholar
  27. 27.
    Plette ACC, van Riemsdijk WH, Benedetti MF and van der Wal A (1995) J Colloid Interface Sci 173:354–363CrossRefGoogle Scholar
  28. 28.
    O'Brien RW, White LR (1978) J Chem Soc Faraday Trans (II) 74:1607–1626CrossRefGoogle Scholar
  29. 29.
    Dukhin SS, Shilov VN (1974) Dielectric Phenomena and the Double layer in Disperse systems and Polyelectrolytes. Wiley, JerusalemGoogle Scholar
  30. 30.
    Fixman M (1983) J Chem Phys 78:1483–1491CrossRefGoogle Scholar
  31. 31.
    Kijlstra J, van Leeuwen HP and Lyklema J (1992) J Chem Soc Faraday Trans 88:3441–3449CrossRefGoogle Scholar
  32. 32.
    Kijlstra J, van Leeuwen HP and Lyklema J (1993) Langmuir 9:1625–1633CrossRefGoogle Scholar
  33. 33.
    Lyklema J (1995) Fundamentals of Interface and Colloid Science, Volume II ch 4. Academic Press (London)Google Scholar
  34. 34.
    Maste MCL (1996) PhD thesis Agricultural University Wageningen NLGoogle Scholar

Copyright information

© Steinkopff Verlag 1996

Authors and Affiliations

  • J. Lyklema
    • 1
  • W. Norde
    • 1
  1. 1.Department of Physical and Colloid ChemistryWageningen Agricultural UniversityWageningenThe Netherlands

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