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Gels pp 19-25 | Cite as

Photochemical activity of the bacterial reaction center in polymer-like phospholipids reverse micelles

  • G. Palazzo
  • M. Giustini
  • A. Mallardi
  • G. Colafemmina
  • M. Della Monica
  • A. Ceglie
Conference paper
Part of the Progress in Colloid & Polymer Science book series (PROGCOLLOID, volume 102)

Abstract

An integral membrane protein, the photosynthetic bacterial reaction center (RC), has been incorporated in reverse micelle viscoelastic gels made of phosphatidylcholine and phosphatidylserine. Due to the dynamic nature of the gels, the use of a technique which shares the same timescale of the charge recombination is advised, in order to correlate the kinetic behaviour of the RC to the hosting-system properties. Self-diffusion and conductivity measurements have been used to investigate the properties of the model system lecithin/cyclohexane/water. The results indicate that such techniques can describe the properties of the system on a long characteristic time-scale. As a consequence, the kinetic behaviour of the RC has been studied by means of flash-spectro-photometry and related to the structural properties of the hosting gel, investigated by means of conductivity. The conductivity data are consistent with a water-induced sphere-to-rod transition of the phospholipid aggregates. Furthermore, increasing the ratio [water]/[lipid], a maximum in the hydrodynamic dimension of the giant worm-like reverse micelles is found. The experimental P+ decay has been resolved into three exponential components which are strongly affected by the system composition. The functionality of the binding site QB is dependent on the ratio [water]/[lipid] supporting the hypothesis of a water role in the binding process.

Key words

Charge-recombination organogels self-diffusion membrane model 

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Copyright information

© Dr. Dietrich Steinkopff Verlag GmbH & Co. KG 1996

Authors and Affiliations

  • G. Palazzo
    • 1
  • M. Giustini
    • 2
  • A. Mallardi
    • 2
  • G. Colafemmina
    • 1
  • M. Della Monica
    • 1
  • A. Ceglie
    • 3
  1. 1.Dipartimento di ChimicaUniversità di BariBariItaly
  2. 2.CNR C. Studi Chimico-Flsici sull’Interazione Luce-MateriaBariItaly
  3. 3.Facoltá di AgrariaUniversità del MoliseCampobassoItaly

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