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Gels pp 9-14 | Cite as

Characterization of helical structures in gelatin networks and model polypeptides by circular dichroism

  • F. A. de Wolf
  • R. C. A. Keller
Conference paper
Part of the Progress in Colloid & Polymer Science book series (PROGCOLLOID, volume 102)

Abstract

Reversible physical crosslinks (junction zones) in gelatin gels and films are thought to consist of triple helical collagen-like structures, however other structures may also occur. In an attempt to understand the relation between the material properties and the various junction structures and their abundance, we characterized these structures using circular dichroism (CD) as a function of the conditions applied. It appeared that the commonly used reference CD spectra for random coil and 31 helix could not be used to deconvolute the spectra observed for gelatin. Most probably, the commonly-used random coil reference spectra do not really reflect unordered structure, while the random coil reference based on the cationic form of polyLysine actually reflects a clear helicity. Denatured gelatin of collagen were found to be better approximations of an unordered polypeptide structure. The prolyl chromophore was shown to have a pronounced influence on the shape and position of the CD spectra. Thus, it was concluded that only polypeptides with the appropriate prolyl content can be used as references, while analysing the relative secondary structure contents by CD. By using temperature-dependent CD, we were able to discern multiple and single helical structures at high and low gelatin concentrations, respectively, by the observed cooperativity of helix-to-coil transition.

Key words

Gelation circular dichroism proline content random coil thermal denaturation model peptide 

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Copyright information

© Dr. Dietrich Steinkopff Verlag GmbH & Co. KG 1996

Authors and Affiliations

  • F. A. de Wolf
    • 1
  • R. C. A. Keller
    • 2
  1. 1.Agrotechnological Research Institute (ATO-DLO)WageningenThe Netherlands
  2. 2.Department of Biochemistry of Membranes Centre for Biomembranes and Lipid EnzymologyUniversity of UtrechtUtrechtThe Netherlands

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