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Solubilization of phosphorylase into microemulsion droplets. An ESR study

  • A. Xenakis
  • C. T. Cazianis
Amphiphile Solutions
Part of the Progress in Colloid & Polymer Science book series (PROGCOLLOID, volume 76)

Abstract

The solubilization of glycogen phosphorylase in different microemulsion systems was studied by ESR spectroscopy. Spin-labelling technique was used to follow the behaviour of this enzyme when hosted in either ionic microemulsion systems formulated with AOT and isooctane or nonionic microemulsion systems with C12EO4 and decane. The differences between the two kinds of systems are discussed in terms of the polarity of the dispersed phase and of the mobilities of the labelled sites of the enzyme molecules. Depending on the composition of the microemulsion systems, the observed variations in both these parameters are shown to be related to structural changes which occur within the microemulsions.

Key words

Microemulsions ESR spectroscopy phosphorylase b ionic systems nonionic systems 

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Copyright information

© Dr. Dietrich Steinkopff Verlag GmbH & Co. KG 1988

Authors and Affiliations

  • A. Xenakis
    • 1
  • C. T. Cazianis
    • 1
  1. 1.Institute of Biological ResearchThe National Hellenic Research FoundationAthensGreece

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