Studies on the solubilization of basic polypeptides in reversed micelles by ultracentrifuge measurements
The sedimentation behavior of reversed micelles (RM) of the system AOT/H2O/iso-octane was studied before and after solubilizing poly(L-lysine).
In contrast to the Schlieren-pattern of the system AOT/H2O/iso-C8, those of aOT/H2O(Lys)n/iso-C8 show two Schlieren peaks. The slowly moving one corresponds to the “empty RM”, the other — with a higher sedimentation rate — to the “filled RM”. From the size of the area below these peaks, it follows that at a maximum ≈ 45% of the RM are filled with (Lys)n. Also, the diffusion patterns show an overlapping of the diffusion process of these two species. The results obtained by the moving boundary method can be confirmed by equilibrium sedimentation measurements.
As a result of these studies, the number of (Lys)n, AOT, and H2O molecules in one RM can be determined. In any case, in the filled RM, only one (Lys)n molecule is solubilized.
Key wordsR eversed micelles sedimenstation behavior polypeptides solubilization conformation
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