Advertisement

Studies on the solubilization of basic polypeptides in reversed micelles by ultracentrifuge measurements

  • G. Ebert
  • M. Plachky
  • M. Seno
  • S. Shoji
Amphiphile Solutions
Part of the Progress in Colloid & Polymer Science book series (PROGCOLLOID, volume 76)

Abstract

The sedimentation behavior of reversed micelles (RM) of the system AOT/H2O/iso-octane was studied before and after solubilizing poly(L-lysine).

In contrast to the Schlieren-pattern of the system AOT/H2O/iso-C8, those of aOT/H2O(Lys)n/iso-C8 show two Schlieren peaks. The slowly moving one corresponds to the “empty RM”, the other — with a higher sedimentation rate — to the “filled RM”. From the size of the area below these peaks, it follows that at a maximum ≈ 45% of the RM are filled with (Lys)n. Also, the diffusion patterns show an overlapping of the diffusion process of these two species. The results obtained by the moving boundary method can be confirmed by equilibrium sedimentation measurements.

As a result of these studies, the number of (Lys)n, AOT, and H2O molecules in one RM can be determined. In any case, in the filled RM, only one (Lys)n molecule is solubilized.

Key words

R eversed micelles sedimenstation behavior polypeptides solubilization conformation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bonner FJ, Wolf R, Luisi PL (1980) J Solid Phase Biochem 5:255–268CrossRefGoogle Scholar
  2. 2.
    Luisi PL (1985) Angew Chem 97:449–460CrossRefGoogle Scholar
  3. 3.
    Ebert Ch, Ebert G, Werner W (1973) Kolloid Z Z Polym 251:504–505CrossRefGoogle Scholar
  4. 4.
    Ebert Ch, Ebert G (1977) Colloid Polym Sci 255:1041–1053CrossRefGoogle Scholar
  5. 5.
    Ebert G (1985) In: Boschke F (ed) Solvation and Ordered Structure in Colloid Systems. Topics in Current Chemistry, Vol 128, pp 1–36Google Scholar
  6. 6.
    Noritomi H (1986) Behaviour of Enzymes and Polypeptides in Reversed Micelles, Thesis, University of Tokyo, JapanGoogle Scholar
  7. 7.
    Plachky M (1987) Untersuchungen der Konformation und Solvatation von Poly-(α-aminosäuren) in inversen Mizellen, Thesis, Philipps-Universität Marburg/Lahn, FRGGoogle Scholar
  8. 8.
    Senō M, Noritomi H, Kuroyanagi Y, Iwamoto K, Ebert G (1984) Colloid Polym Sci 262:727–733CrossRefGoogle Scholar

Copyright information

© Dr. Dietrich Steinkopff Verlag GmbH & Co. KG 1988

Authors and Affiliations

  • G. Ebert
    • 3
  • M. Plachky
    • 3
  • M. Seno
    • 1
    • 3
  • S. Shoji
    • 2
    • 3
  1. 1.Institute of Industrial ScienceUniversity of TokyoTokyoJapan
  2. 2.Department of Industrial Chemistry, College of TechnologyGunma UniversityGunmaJapan
  3. 3.Fachbereich Physikalische ChemiePhilipps-UniversitätMarburg a. d. LahnF.R.G.

Personalised recommendations