Abstract
Mutual adsorption of bovine serum albumin (BSA) and sodium dodecyl sulphate (SDS) at the alumina-water interface has been determined as functions of protein and detergent concentrations, ionic strength, pH and temperature of the aqueous medium. The extgent of adsorption decreases with increase of temperature. The isotherm with respect to the adsorption of protein at a fixed total concentration (C ts ) of the surfactant is S-shaped in character. The limiting value of the protein adsorption decreases with increase of C s/t. The study of adsorption at serveral values of pH and ionic strength of the medium indicates that the mutual interactions of BSA and SDS with alumina interface are significantly controlled byt ehe electrostatic effect. At a given value of C ts , extent of adsorption (Γ S) at first decreases with increase of the adsorption (Γ p) of BSA due to the competitive interaction process whereas Γ S increases with increases of Γ p in the relatively higher range of the adsorption of protein as a result of the increase of the mutual interaction of BSA and SDS at the interface. The binding ratio of SDS to BSA in the bulk phase and at the aluminawater interface respectively under identical situation have been compared at various values of the equilibrium bulk concentrations (C s) of the surfactant in solution. At low values of C s, these ratios in the two phases are of comparable order in magnitude. However, at high values of C s, the binding ratio at the interface increases enormously due to the massive cooperative interaction even though the value of this ratio in the bulk remains low.
This is a preview of subscription content, log in via an institution.
Preview
Unable to display preview. Download preview PDF.
References
Tamamushi, B., Tamaki, K., “Proceedings of the Second International Congress of Surface Activity”, Butterworths, London, 1957. Vol. III, p. 449.
Tamamushi, B., Tamaki, K., Trans. Faraday Soc. 55, 1007 (1959).
Connor, P., Ottewill, R. H., J. Colloid Interface Sci. 37, 595 (1964).
Mukherjee, P., Anavil, A., in: “Adsorption at Interfaces” edited by K. L. Mittal, ACS Symposium Series No. 8, American Chemical Society, Washington D. C., p. 107 (1975).
Sommasundaran, P. and Fuerstenau, D. H., J. Phys. Chem. 70, 90 (1966).
Sommasundaran, P., Healy, T. W., Fuerstenau, D. H., J. Phys. Chem. 68, 3562 (1964).
Trogor, F. J., Schechter, R. S., Wade, W. H., J. Colloid Interface Sci. 70, 293 (1979).
Bull, H. B., Biochem. Biophys. Acta 19, 464 (1956).
Bull, H. B., Archs. Biochem. Biophys. 68, 102 (1957).
Chattoraj, D. K. and Bull, H. B., J. Amer. Chem. Soc., 81, 5128 (1959).
Dilman, W. J., Miller, I. R., J. Colloid Interface Sci. 44, 221 (1974).
Morrissey, B. W., Stromberg, R. R., J. Colloid Interface Sci. 44, 221 (1974).
Mitra, S. P., Chattoraj, D. K., Indian J. Biochem. Biophys. 15, 147 (1978).
Norde, W., Lyklema, J., J. Colloid Interface Sci. 66, 257, 266, 277, 285, 295 (1978).
Norde, W., “Proteins at Interfaces”, Doctoral Thesis, Agricultural University, Wageningen, (Netherlands, 1976).
Schulman, J. H. and Rideal, E. K., Proc. Roy. Soc. Ser. B, 122, 46 (1937).
Eley, D. D., Hedge, D. G., J. Colloid Sci. 11, 445 (1957).
Bull, H. B., J. Amer. Chem. Soc. 67, 10 (1945).
Pearson, J. T., J. Colloid Interface Sci. 27, 64 (1968).
Arnebrant, T., Nylander, T., Hegg, p. O., Larson, Kara, Abstracts, “International Symposium on Surfactants in Solution”, June-–July, 1982, Lund, Sweden, page 30.
Mukherjee, P., Mysels, K. J., “Critical Micelle concentration”, National Bureau of Standards, (Washington D. C., 1971).
Chatterjee, R., Chattoraj, D. K., Biopolymer 18, 147 (1979).
Sen, M., Mitra, S. P., and Chattoraj, D. K., Colloids and Surfaces 2, 259 (1981).
Sen, M., Mitra, S. P., Chattoraj, D. K., Indian J. Biochem. Biophys. 17, 370 (1980).
Mukherjee, P., Anal. Chem. 28, 870 (1956).
Biswas, H. K., Mondal, B. M., Anal. Chem. 44(9), 1636 (1972).
Orr, C., Dallavalle, J. M., Fine Particle Measurement: Size, Surface and Pore Volume, pp. 207–215, The McMillar Company (New York, 1959).
Chattoraj, D. K., Chowrashi, P., Chakravarti, K., Biopolymer 5, 173 (1967).
Spahr, P. F., Edsall, J. T., J. Biol. Chem. 239, 850 (1964).
Tanford, C., “Hydrophobic Effect,” Formation of Micelles and Biological Membranes, Wiley, (New York, 1980).
Author information
Authors and Affiliations
Editor information
Additional information
Dedicated to the memory of Professor Dr. B. Tamamushi.
Rights and permissions
Copyright information
© 1983 Dr. Dietrich Steinkopff Verlag GmbH & Co. KG
About this paper
Cite this paper
Samanta, A., Chattoraj, D.K. (1983). Mutual adsorption of protein and detergent at the alumina-water interface. In: Nakagaski, M., Shinoda, K., Matijević, E. (eds) Frontiers in Colloid Science In Memoriam Professor Dr. Bun-ichi Tamamushi. Progress in Colloid & Polymer Science, vol 68. Steinkopff. https://doi.org/10.1007/BFb0114152
Download citation
DOI: https://doi.org/10.1007/BFb0114152
Received:
Accepted:
Published:
Publisher Name: Steinkopff
Print ISBN: 978-3-7985-0637-4
Online ISBN: 978-3-7985-1703-5
eBook Packages: Springer Book Archive