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Sedimentation studies of specific association of oligonucleosomes from sea urchin sperm chromatin

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Book cover Analytical Ultracentrifugation

Part of the book series: Progress in Colloid & Polymer Science ((PROGCOLLOID,volume 99))

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Abstract

It is known that oligonucleosomal chromatin fragments from chicken erythrocytes enriched in inactive genes can self-associate into pseudo-higher-order structures. In this paper, we demonstrate the similar aggregation phenomenon for another type of repressed cells, sea urchin spermatozoa. Like the inactive part of erythrocyte chromatin, sea urchin sperm chromatin is characterized by the long linker DNA (100 base pairs) and the presence of specific histones typical for the repressed state of chromatin. It seems that these two factors determine the specific association of oligonucleosomes. The aggregates of sea urchin sperm oligonucleosomes resemble those of the erythrocyte chromatin in the following features: the range of the sizes of oligonucleosomes that are able to associate into pseudo-higher-order structures is limited; such self-association takes place at a low extent of the chromatin digestion by micrococcal nuclease; the aggregates undergo salt-induced compaction. Similarity in the properties of chromatin aggregates from two types of terminally repressed cells makes it possible to consider the specific association into pseudo-higher-order structures as a characteristic feature of chromatin from the inactive part of the genome.

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Author notes

  1. T. N. Osipova

    Present address: Biological Institute of the St. Petersburg University, 198904, St. Petersburg, Russia

Authors and Affiliations

  1. Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky pr. 4, 194064, St. Petersburg, Russia

    E. V. Karpova, T. N. Osipova & V. I. Vorob’ev

Authors
  1. E. V. Karpova
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  2. T. N. Osipova
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  3. V. I. Vorob’ev
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Editor information

J. Behlke

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© 1995 Dr. Dietrich Steinkopff Verlag GmbH & Co. KG

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Karpova, E.V., Osipova, T.N., Vorob’ev, V.I. (1995). Sedimentation studies of specific association of oligonucleosomes from sea urchin sperm chromatin. In: Behlke, J. (eds) Analytical Ultracentrifugation. Progress in Colloid & Polymer Science, vol 99. Steinkopff. https://doi.org/10.1007/BFb0114070

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  • DOI: https://doi.org/10.1007/BFb0114070

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  • Publisher Name: Steinkopff

  • Print ISBN: 978-3-7985-1038-8

  • Online ISBN: 978-3-7985-1666-3

  • eBook Packages: Springer Book Archive

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