Skip to main content
SpringerLink
Log in

Investigations of the oligometric state of the 42 kDa repressor isoform from the streptomcyes temperate bacteriophage φC31

  • Conference paper
  • First Online:
Analytical Ultracentrifugation

Part of the book series: Progress in Colloid & Polymer Science ((PROGCOLLOID,volume 99))

  • 133 Accesses

Abstract

The repressor gene from the Streptomyces phage φC31 expresses three N-terminally different, in-frame protein isoforms of polypeptide molecular masses 74, 54 and 42 kDa. Their precise role in the lysis versus lysogeny decision is currently being investigated and a study of their structural and interactive properties was considered an important aid in this investigation. The preliminary data presented here shows that the native 42 kDa isoform and a Histidine (His-)-tagged form exist as dimers or tetramers, depending on the conditions as determined by sedimentation equilibrium in the analytical ultracentrifuge.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Smith, MCM, Owen CE (1991) Mol Microbiol 5:2833–2844

    Article  CAS  Google Scholar 

  2. Ingham CJ, Owen CE, Wilson SE, Hunter IS, Smith MCM (1994) Nucleic Acids Res 22:821–827

    Article  CAS  Google Scholar 

  3. Wilson SE, Ingham CJ, Hunter IS, Smith MCM (1995) Mol Microbiol 16:131–143

    Article  CAS  Google Scholar 

  4. Wilson SE, Smith MCM unpublished results

    Google Scholar 

  5. Broughton D, Smith MCM, unpublished results

    Google Scholar 

  6. Creeth JM, Harding SE (1982) J Biochem Biophys Methods 7:25–34

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Applied Biochemistry and Food Science, National Centre for Macromolecular Hydrodynamics The University of Nottingham, Le12 5RD, Sutton Bonington, United Kingdom

    K. Jumel & S. E. Harding

  2. Department of Genetics, Queens Medical Centre, NG7 UH, University Park, Nottingham, United Kingdom

    S. E. Wilson & M. C. M. Smith

Authors
  1. K. Jumel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. E. Wilson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. C. M. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. E. Harding
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

J. Behlke

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Dr. Dietrich Steinkopff Verlag GmbH & Co. KG

About this paper

Cite this paper

Jumel, K., Wilson, S.E., Smith, M.C.M., Harding, S.E. (1995). Investigations of the oligometric state of the 42 kDa repressor isoform from the streptomcyes temperate bacteriophage φC31. In: Behlke, J. (eds) Analytical Ultracentrifugation. Progress in Colloid & Polymer Science, vol 99. Steinkopff. https://doi.org/10.1007/BFb0114064

Download citation

  • DOI: https://doi.org/10.1007/BFb0114064

  • Received:

  • Accepted:

  • Published:

  • Publisher Name: Steinkopff

  • Print ISBN: 978-3-7985-1038-8

  • Online ISBN: 978-3-7985-1666-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation