Skip to main content
SpringerLink
Log in

Small-angle neutron scattering study on short-chain phosphatidylcholine micelle in the presence of sorbitol

  • Biocolloids
  • Conference paper
  • First Online:
Formation and Dynamics of Self-Organized Structures in Surfactants and Polymer Solutions

Part of the book series: Progress in Colloid & Polymer Science ((PROGCOLLOID,volume 106))

Abstract

Sorbitol is a natural cryoprotectant for some organisms. In order to reveal the mechanism of a protection some organisms against low temperatures, we studied the interaction between sorbitol and phosphatidylcholine (PC) which is one of major component of cell membranes. Small-angle neutron-scattering contrast variation method was used to characterize dihexanoyl-PC (dC(6)PC) micelles in the presence of sorbitol (2 M). As a result, the aggregation number for the dC(6)PC micelle and the volume per a single molecule in the micelle were estimated to be ∼32 and ∼640 Å3, respectively. By comparing these data and those for the dC(6)PC micelle in pure water (Lin et al, J Am Chem Soc 108:3499–3507), it was concluded that sorbitol reduces the contact area between the lipid and the aqueous phase. On the basis of this conclusion, we discuss the role of sorbitol for the ability of some organisms to survive in low temperatures.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Crowe JH, Crowe LM, Carpenter JF, Wistrom CA (1987) Biochem J 242:1–10

    CAS  Google Scholar 

  2. Storey KB, Baust JG Storey JM (1981) J Comp Physiol 144:183–190

    CAS  Google Scholar 

  3. Chowdhry BZ, Lipka G, Sturtevant JM (1984) Biophys J 46:419–422

    Article  CAS  Google Scholar 

  4. Tsvekov TD, Tsonev LI, Tsvekova NM, Koynova RD, Tenchov BG (1989) Cryobiology 26:162–169

    Article  Google Scholar 

  5. Koynova RD, Tenchov BG, Quinn PJ (1989) Biochim Biophys Acta 980:377–380

    Article  CAS  Google Scholar 

  6. Bryszewska M, Epand RM (1988) Biochem Biophys Acta 943:485–492

    Article  CAS  Google Scholar 

  7. Sanderson PW, Lis LJ, Quinn PQ, Williams WP (1991) Biochem Biophys Acta 1067:43–50

    Article  CAS  Google Scholar 

  8. Tsonev LI, Tihova MG, Brain APR, Yu ZW. Quinn PJ (1994) Liq Cryst 17:717–728

    Article  CAS  Google Scholar 

  9. Tausk RIM, Karmiggelt J, Oudshoorn C, Overbeek JThG (1947) Biophys Chem 1:175–183

    Article  Google Scholar 

  10. Burns Jr RA, Roberts MF, Dluhy R, Mendelsohn R (1982) J Am Chem Soc 104:430–438

    Article  CAS  Google Scholar 

  11. Lin, TL. Chen SH, Gabriel NE, Roberts MF (1986) J Am Chem Soc 108:3499–3507

    Article  CAS  Google Scholar 

  12. Lin TL, Chen SH. Gabriel NE, Roberts MF (1987) J Phys Chem 91:406–413

    Article  CAS  Google Scholar 

  13. Magid LG, Trioio R, Johonson Jr JS, Koehler WC (1982) J Phys Chem 86:164–167

    Article  CAS  Google Scholar 

  14. Bendedouch D, Chen SH, Koehler WC (1983) J Phys Chem 87:153–159

    Article  CAS  Google Scholar 

  15. Kawaguchi T, Hamanaka T, Kito Y, Machida H (1991) J Phys Chem 95:3837–3846

    Article  CAS  Google Scholar 

  16. Kawaguchi T, Hamanaka T (1992) J Colloid Interface Sci 151:41–48

    Article  CAS  Google Scholar 

  17. Ito Y, Imai M, Takahashi S (1995) Physica B 213/214:889–891

    Article  Google Scholar 

  18. Imai M, Kaji K, Kanaya T. Sakai Y (1995) Rev B 52:12696–12704

    Article  CAS  Google Scholar 

  19. Guinier A, Fournet G (1995) In: Small Angle Scattering of X-rays. Wiley. New York

    Google Scholar 

  20. Aarnett EA, Harvey N, Johnson EA, Johnston DS, Chapman D (1986) Biochemistry 25:5239–5242

    Article  Google Scholar 

  21. Privalov P, Gill SJ (1988) Adv Protein Chem 39:191–231

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Applied Physics, Nagoya University, Chikusa-ku, 464-01, Nagoya, Japan

    Dr. H. Takahashi & I. Hatta

  2. Neutron Scattering Laboratory Institute of Solid State Physics, Tokyo University, Tokai, Japan

    M. Imai & Y. Matsushita

Authors
  1. Dr. H. Takahashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Imai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. Matsushita
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. Hatta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to H. Takahashi .

Editor information

K. Kawasaki B. Lindman H. Okabayashi

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Steinkopff Verlag

About this paper

Cite this paper

Takahashi, H., Imai, M., Matsushita, Y., Hatta, I. (1997). Small-angle neutron scattering study on short-chain phosphatidylcholine micelle in the presence of sorbitol. In: Kawasaki, K., Lindman, B., Okabayashi, H. (eds) Formation and Dynamics of Self-Organized Structures in Surfactants and Polymer Solutions. Progress in Colloid & Polymer Science, vol 106. Steinkopff. https://doi.org/10.1007/BFb0111063

Download citation

  • DOI: https://doi.org/10.1007/BFb0111063

  • Received:

  • Accepted:

  • Published:

  • Publisher Name: Steinkopff

  • Print ISBN: 978-3-7985-1096-8

  • Online ISBN: 978-3-7985-1659-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation