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The potential of using cyanobacteria in photobioreactors for hydrogen production

  • S. A. Markov
  • M. J. Bazin
  • D. O. Hall
Chapter
Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 52)

Abstract

This review surveys data on cyanobacterial hydrogen photoproduction with a view to a use of cyanobacteria in photobioreactors for hydrogen production.

Three groups of cyanobacteria can be distinguished based on their hydrogen producing physiological characteristics: a) heterocystous filamentous, b) nonheterocystous filamentous and c) nonheterocystous unicellular strains. Information on certain metabolically unique strains of cyanobacteria which have special promise for practical hydrogen production is reviewed.

Environmental parameters and physiological factors which might be of use to optimize cyanobacterial hydrogen generation are identified and summarized. These parameters include: light intensity, gas atmosphere (CO2, N2 and O2), temperature, pH, carbohydrate substrates, metal ions, H2 uptake systems, age of cyanobacterial culture, cell density, and immobilization of cells.

Immobilization of cyanobacterial cells in polyurethane or polyvinyl foams, hollow fibres, glass beads and cotton, leads to an increase/or stabilization for several months or more of hydrogen production. Cell immobilization results in an increased heterocyst frequency (up to 40%). Immobilized cells in comparison with free living cells are likely to be more suitable for hydrogen production in photobioreactors.

Nitrogenase is a major catalytic enzyme for hydrogen production in cyanobacteria, which can express three distinct nitrogenases: molybdenum nitrogenase, vanadium nitrogenase and iron nitrogenase. Details of the biochemical manipulation of nitrogenase-catalyzed hydrogen photo-production is reviewed. In the case of molybdenum-independent nitrogenases, hydrogen production is significantly higher than for molybdenum-containing nitrogenase. Cyanobacterial hydrogenase is an enzyme which catalyzes both hydrogen evolution and hydrogen uptake. Several means to inhibit hydrogenase uptake activity are described. Removal of H2 from the cyanobacterial cell environment in a continuous-flow photobioreactor is an effective method of preventing hydrogen uptake. Cyanobacteria may also be genetically modified for deletion of the uptake hydrogenase gene. Other objectives of genetic research related to cyanobacterial hydrogen photoproduction are reviewed.

A number of reports on cyanobacterial photobioreactors for hydrogen production are described. Such photobioreactors can be operated continuously for several months or more. Under outdoor conditions H2-producing photobioreactors operated at good efficiencies. For example a 51 outdoor photobioreactor with Oscillatoria sp. produced hydrogen for a week at rates of 8.2 ml g−1 h−1.

Particular attention in the review is given to fixed bed hollow fibre column photobioreactors. Several advantages of those constructions are characterized. Photoproduction of hydrogen at rates up to 200 ml g−1 h−1 in a photobioreactor with hollow fibre immobilized Anabaena variabilis was observed for more than 5 months. An example of a computer-controlled equipment system for cyanobacterial growth is given.

Keywords

Hydrogen Production Hollow Fibre Hydrogen Production Rate MoFe Protein Heterocystous Cyanobacterium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • S. A. Markov
    • 1
  • M. J. Bazin
    • 1
  • D. O. Hall
    • 1
  1. 1.Environmental Biotechnology Research Group, Division of Life SciencesKing's College LondonLondonUK

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