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Chromatography pp 147-160 | Cite as

Liquid chromatography using cellulosic continuous stationary phases

  • Yiqi Yang
  • Ajoy Velayudhan
  • Christine M. Ladisch
  • Michael R. Ladisch
Chapter
Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 49)

Abstract

A novel type of continuous stationary phase based on fabric materials is described. This column packing utilizes the continuous character of a cellulose (cotton) stationary phase, and the chemistry of the derivatized forms of the adsorbent, to obtain separations of proteins and small molecules based on cation and anion exchange, hydrophobic interactions, and size. The mechanical stability of the stationary phase facilitates chromatographic velocities in excess of 70 cm min−1. The influence of eluent properties on the adsorption of sample proteins is discussed in this chapter. Sequential stepwise desorption is used to separate 100 μl mixtures of BSA, IgG, β-galactosidase, and insulin in 10 minutes or less, using 10 mm i.d. × 500 mm length columns.

Keywords

Bovine Serum Albumin Hollow Fiber DEAE Cellulose C2H5 Cell Ethyl Chloride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Yiqi Yang
    • 1
  • Ajoy Velayudhan
    • 2
  • Christine M. Ladisch
    • 1
  • Michael R. Ladisch
    • 2
    • 3
  1. 1.Department of Consumer Sciences and RetailingPurdue UniversityWest LafayetteUSA
  2. 2.Laboratory of Renewable Resources EngineeringPurdue UniversityWest LafayetteUSA
  3. 3.Department of Agricultural EngineeringPurdue UniversityWest LafayetteUSA

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