Skip to main content
SpringerLink
Log in
Book cover

Reviews of Physiology, Biochemistry and Pharmacology, Volume 79 pp 133–162Cite as

H+ transport by a non-electrogenic gastric ATpase as a model for acid secretion

  • Chapter
  • First Online:

Part of the book series: Reviews of Physiology, Biochemistry and Pharmacology ((REVIEWS,volume 79))

Abstract

It would appear, therefore, that hog gastric mucosa contains a particularly interesting system which in many respects is compatible with a model for H+ secretion. It is quite different, at current levels of analysis, from the H+ ATPase of mitochondria. Indeed the stomach appears to have developed a unique method of developing H+ gradients. The magnitude of this gradient is such that perhaps this ATPase is only partially responsible, and that a second pump, in series with the one discussed in some detail here, is required for the complete process.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Mitchell P.: Coupling of phosphorylation to electron and hydrogen transfer by a chemiosmotic type of mechanism. Nature (Lond.) 191, 144–148 (1961)

    Article  PubMed  CAS  Google Scholar 

  2. Jagendorf, A. T., Uribe, E.: ATP formation caused by acid-base transition of spinach chloroplasts. Proc. nat. Acad. Sci (Wash.) 55, 170–177 (1966)

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  3. Kagawa Y., Racker E.: Partial resolution of the enzymes catalyzing oxidative phosphorylation. XXV. Reconstitution of vesicles catalyzing 32Pi-ATP exchange. J. biol. Chem. 246, 5477–5487 (1971)

    CAS  Google Scholar 

  4. Racker E., Stoeckenius W.: Reconstitution of purple membrane vesicles catalyzing light driven H+ uptake and ATP formation. J. biol. Chem. 249, 662–666 (1974)

    PubMed  CAS  Google Scholar 

  5. Hersey S. J.: Interaction between oxidative metabolism and acid secretion in gastric mucosa. Biochim. biophys. Acta 244, 157–203 (1974)

    Article  Google Scholar 

  6. Senior A. E.: The structure of mitochondrial ATPase. Biochim. biohys. Acta 301, 249–277 (1973)

    Article  CAS  Google Scholar 

  7. MacLennan D. H., Tzagoloff A.: Studies on the mitochondrial adenosine triphosphatase system. IV. Purification and characterization of the oligomycin sensitivity conferring protein. Biochemistry 7, 1603–1609 (1968)

    Article  PubMed  CAS  Google Scholar 

  8. Pullman, H. W., Monroy, G. C.: A naturally occurring inhibitor of mitochondiral ATPase. J. biol. Chem. 238, 2762–2769 (1963)

    Google Scholar 

  9. Hinkle P. C., Horstman L. L.: Respiration driven proton transport in submitochondrial particles. J. biol. Chem. 246, 6024–6028 (1971)

    PubMed  CAS  Google Scholar 

  10. Mitchell, P., Moyle, J.: Mechanism of proton translocation in reversible proton translocating ATPases. Biochemistry 4, 91–111 (1974)

    CAS  Google Scholar 

  11. Holloway, C. T., Roberton, A. M., Knight, I. G., Beechey, R. B.: The binding of [14C] dicyclohexylcarbodiimide to mitochondrial fractions. Biochem. J. 100, 79 (1966)

    Google Scholar 

  12. Kroman, E. F., McLick, J.: ATP synthesis in oxidative phosphorylation: A direct-union stereochemical reaction mechanism. J. Bioenerg. 3, 147–158 (1972)

    Article  Google Scholar 

  13. Skou, J. C.: Enzymatic basis for active transport of Na+ and K+ across cell membrane. Physiol. Rev. 45, 596–617 (1965)

    PubMed  CAS  Google Scholar 

  14. Martonosi, A.: Transport of calcium by the sarcoplasmic reticulum. In: Metabolic Transport. Hokin, L. E. (ed.). New York: Academic Press, 1972, Vol VI, p. 317–349

    Google Scholar 

  15. Ganser, A. L., Forte, J. G.: K+ stimulated ATPase in purified microsomes of bullfrog oxyntic cells. Biochim. biophys. Acta 307, 169–180 (1973)

    Article  PubMed  CAS  Google Scholar 

  16. Degani, G., Boyer, P. D.: A borohydride reduction method for characterization of the acyl phosphate linkage in proteins and its application to sarcoplasmic reticulum ATPase. J. biol. Chem. 248, 8222–8226 (1973)

    PubMed  CAS  Google Scholar 

  17. Nishigaki, T., Chen, F. T., Hokin, L. E.: Studies on Na + K ATPase. XV. Direct chemical characterization of acyl phosphate in the enzyme as β-aspartyl phosphate. J. biol. Chem. 249, 4911–4916 (1974)

    PubMed  CAS  Google Scholar 

  18. Post, R. L., Merritt, C. R., Kinsolving, C. R., Albright, C. D.: Membrane adenosine triphosphatase as a participant in the active transport of sodium and potassium in the human erythrocyte. J. biol. Chem. 235, 1796–1802 (1960)

    PubMed  CAS  Google Scholar 

  19. Fahn, S., Hurley, J. R., Koral, G. J., Albers, R. W.: Sodium potassium activated adenosine triphosphatase of electrophoresis electric organ. J. biol. Chem. 241, 1890–1895 (1966)

    PubMed  CAS  Google Scholar 

  20. Taniguchi, K., Post, R. L.: Synthesis of ATP and exchange between Pi and ATP in Na++K+ ATPase. J. biol. Chem. 250, 3010–3018 (1973)

    Google Scholar 

  21. Kepner, G. R., Macey, R. I.: Membrane enzyme systems. Biochim. biophys. Acta 163, 188–203 (1968)

    Article  PubMed  CAS  Google Scholar 

  22. Goldin, S. N., Tong, J. W.: Reconstitution of active ion transport by the Na+K ATPase. J. biol. Chem. 249, 5902–5915 (1975)

    Google Scholar 

  23. Shamoo, A. E., Ryan, T. E.: Isolation of ionophores from ion transport systems. Ann. N. Y. Acad. Sci. 264, 83–97 (1975)

    Article  PubMed  CAS  Google Scholar 

  24. Racker, E., Knowles, A. F., Eytan, E.: Resolution and reconstitution of ion transport system. Ann. N. Y. Acad. Sci. 264, 17–33 (1975)

    Article  PubMed  CAS  Google Scholar 

  25. Glynn, I. M., Karlish, J. D.: Different approaches to the mechanism of the sodium pump. In: Energy Transformation in Biological System, 205–224 (1975) Ciba Geigy

    Google Scholar 

  26. Forte, J. G., Forte, G. M., Saltman, P.: K+ stimulated phosphatase of microsomes from gastric mucosa. J. cell. comp. Physiol. 69, 293–304 (1967)

    Article  CAS  Google Scholar 

  27. Sachs, G., Rose, J. D., Shoemaker, R. I., Hirschowitz, B. I.: Phosphatase reactions of transport ATPase. Physiologist, 9, 281 (1966)

    Google Scholar 

  28. Sachs, G., Rose, J. D., Hirschowitz, B. I.: Acetyl phosphatase in rat brain microsomes. Arch. Biochem. 119, 277–281 (1967)

    Article  PubMed  CAS  Google Scholar 

  29. Albers, R. W., Arnaiz, G. R. L., De Robertis, E.: Sodium-potassium-activated ATPase and potassium-activated p-nitrophenylphosphatase: A comparison of their subcellular localizations in rat brain. Proc. nat Acad. Sci. (Wash.) 53, 557–564 (1965)

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  30. Ganser, A. L., Tanisawa, A. S., Forte, J. G.: The K+-stimulated ATPase system of microsomal membranes from gastric oxyntic cells. Ann. N. Y. Acad. Sci. 242, 255–267 (1974)

    Article  PubMed  Google Scholar 

  31. Forte, J. G., Ganser, A., Beesly, R., Forte, T. M.: Unique enzymes of purified microsomes from pig fundic mucosa. Gastroenterology 69, 175–189 (1975)

    PubMed  CAS  Google Scholar 

  32. Spenney, J. G., Saccomani, G., Spitzer, H. L., Sachs, G.: Characterization of gastric mucosal membranes. Arch. Biochem. biophys. Acta 161, 456–471 (1974)

    Article  CAS  Google Scholar 

  33. Saccomani, G., Stewar, H.B., Shaw, D., Lewin, M., Sachs, G. Fractionation and purification of K+ ATPase containing vesicles. Biochim. biophys. Acta 465, 311–330 (1977)

    Article  PubMed  CAS  Google Scholar 

  34. Saccomani, G., Shah, G., Spenney, J. G., Sachs, G.: Localization of peptides by iodination and phosphorylation. J. biol. Chem. 250, 4802–4809 (1975)

    PubMed  CAS  Google Scholar 

  35. Saccomani, G., Rabon, E., Sachs, E.: In preparation.

    Google Scholar 

  36. Sachs, G., Hirschowitz, B. I.: (1968) Secretion by in vitro amphibian mucosa IN: Physiology of Gastric Secretion. Myren, J. (ed.). Oslo: Oslo University Press, 1968, pp. 186–202

    Google Scholar 

  37. Jarnefelt, J., Stedingk, L. V. von: Some properties of the ATP dependent Na binding system of rat brain microsomes. Acta physiol. scand 57: 328–338 (1963)

    Article  PubMed  CAS  Google Scholar 

  38. Sachs, G., Shoemaker, R. L., Blum, A. L., Helander, H. F., Makhlouf, G. M., Hirschowitz, B. I.: (1971) Microelectrode studies of gastric mucosa. IN: Electrophysiology of Epithelial Cells. Giebisch, G. (ed.) pp. 257–279

    Google Scholar 

  39. Ganser, A. L., Forte, J. G.: Ionophoretic stimulation of K+ ATPase of oxyntic cell microsomes. Biochem. Biophys. Res. Comm. 54, 690–696 (1973)

    Article  PubMed  CAS  Google Scholar 

  40. Lee, J., Simpson, E., Scholes, P.: Changes of outer pH in suspension of membrane vesicles accompanying ATP hydrolysis. Biochem. Biophys. Res. Commun. 60, 825–834 (1974)

    Article  PubMed  CAS  Google Scholar 

  41. Sachs, G., Rabon, E., Saccomani, G., Sarau, H. M.: Redox and ATP in acid secretion. Ann. N. Y. Acad. Sci. 264, 456–475 (1975)

    Article  PubMed  CAS  Google Scholar 

  42. Sachs, G., Hung, H., Rabon, E., Schackmann, R., Lewin, M., Saccomani, G. A non-electrogenic H+ pump in plasma membranes of hog stomach. J. biol. Chem. 251, 7690–7698 (1976)

    PubMed  CAS  Google Scholar 

  43. Alberty, R. A.: Standard Gibbs free energy, enthalpy and entropy changes as a function of pH and pMg for several reactions involving adenosine phosphates. J. biol. Chem. 244, 3290–3302 (1969)

    PubMed  CAS  Google Scholar 

  44. Martin, V. S., Sokolov, J. S., Boguslavsky, L. I., Janskinsky, L. S.: Nigericin induced charge transfer across membranes. J. Membr. Biol. 25, 23–46 (1973)

    Google Scholar 

  45. Witt, T. H.: Primary acts of energy conservation in the functional membrane of photosynthesis. (1974) Ann. N. Y. Acad. Sci. 227, 203–206 (1974)

    Article  PubMed  CAS  Google Scholar 

  46. Brand, M. D., Reynafarje, B., Lehninger, A. L.: Stoichiometric relationship between H+ and electron transport. Proc. nat. Acad. Sci. (Wash.) 73, 437–441 (1976)

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  47. Schackmann, R., Schwartz, A., Saccomani, G., Sachs, G.: Cation transport by gastric H++K+ ATPase. J. Membr. Biol. 32, 361–381 (1977)

    Article  PubMed  CAS  Google Scholar 

  48. Scarborough, G. A.: The neurospora plasma membrane ATPase is an electrogenic pump. Proc. nat. Acad. Sci. 73, 1485–1488 (1976)

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  49. Azzi, A., Gheradini, P., Santato, P.: Flurochrome interaction with the mitochondrial membrane. J. biol. Chem. 246, 2035–2042 (1971)

    Google Scholar 

  50. Lewin, M., Saccomani, G., Schackmann, R., Sachs, G.: Use of ANS as a probe of gastric vesicle transport. J. Membr. Biol. in press 32, 301–318 (1977)

    Article  PubMed  CAS  Google Scholar 

  51. Goodall, M. C., Sachs, G.: Reconstitution of a proton pump. J. Membr. Biol. in press (1977)

    Google Scholar 

  52. Hogben, C.A.M.: The Cl− transport system of gastric mucosa. Proc. nat. Acad. Sci. 37, 343–395 (1951)

    Article  Google Scholar 

  53. Sachs, G., Shoemaker, R. L., Hirschowitz, B. I.: Effect of Na removal from in vitro frog gastric mucosa. Proc. Soc. exp. Biol. (N. Y.) 123, 47–52 (1966)

    Article  PubMed  CAS  Google Scholar 

  54. Heinz, E., Durbin, R. P.: Evidence for an independent H+ ion pump in the stomach. Biochim. biophys. Acta 31, 246–247 (1959)

    Article  PubMed  CAS  Google Scholar 

  55. Sarau, H. M., Foley, J., Moonsamy, G., Wiebelhaus, V. D., Sachs, G.: Metabolism of dog gastric mucosa. J. biol. Chem. 250, 8321–8329 (1975)

    PubMed  CAS  Google Scholar 

  56. Durbin, R. P., Michelangeli, F., Michel, A.: Active transport and ATP in frog gastric mucosa. Biochim. biophys. Acta 357, 177–189 (1974)

    Article  Google Scholar 

  57. Sedar, A. W.: Fine structure of the stimulated oxyntic cell. Fed. Proc. 24, 1360–1367 (1965)

    PubMed  CAS  Google Scholar 

  58. Helander, H. F., Hirschowitz, B. I.: Quantitative ultrastructural studies on gastric parietal cells. Gastroenterology 63, 951–961 (1972)

    PubMed  CAS  Google Scholar 

  59. Harris, J. B., Frank, H., Edelman, I. S.: Effect of K+ on ion transport and biolectric potentials of frog gastric mucosa. Amer. J. Physiol. 195, 499–504 (1958)

    PubMed  CAS  Google Scholar 

  60. Rehm, W. S., Sanders, S. S., Rutledge, J. R., David, T. L., Kurfee, J. F., Keesee, D. C., Bajandas, F. J.: Effect of removal of external K+ on frog stomach in Cl− free solutions. Amer. J. Physiol. 210, 689–693 (1966)

    PubMed  CAS  Google Scholar 

  61. Hirschowitz, B. I., Sachs, G.: Insulin inhibition of gastric secretion reversal by Rb+. Amer. J. Physiol. 213, 1401–1405 (1967)

    PubMed  CAS  Google Scholar 

  62. Sachs, G., Collier, R. H., Pacifico, A., Hirschowitz, B. I.: Action of SCN− on gastric mucosa in vitro. Biochim. biophys. Acta 173, 504–507 (1969)

    Article  Google Scholar 

  63. Rehm, W. S.: Electrophysiology of gastric mucosa in Cl− free solutions. Fed. Proc. 24, 1387–1395 (1965)

    PubMed  CAS  Google Scholar 

  64. Sachs, G., Mihas, A., Crago, S., Saccomani, G.: Localization of a gastric H+ pump component. Gastroenterology Abstr. 72, 1125 (1977)

    Google Scholar 

  65. Rabon, E.C., Sarau, H.M., Rehm, W.S., Sachs, G.: Redox involvement in acid secretion in the amphibian gastric mucosa. J. Membr. Biol. in press (1977)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Membrane Biology, University of Alabama in Birmingham, Birmingham, Alabama, USA

    G. Sachs

Authors
  1. G. Sachs
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 1977 Springer-Verlag

About this chapter

Cite this chapter

Sachs, G. (1977). H+ transport by a non-electrogenic gastric ATpase as a model for acid secretion. In: Reviews of Physiology, Biochemistry and Pharmacology, Volume 79. Reviews of Physiology, Biochemistry and Pharmacology, vol 79. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0037090

Download citation

  • DOI: https://doi.org/10.1007/BFb0037090

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-08326-9

  • Online ISBN: 978-3-540-37309-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation