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Controlled release of immobilized cells as a strategy to regulate ecological competence of inocula

  • Aiden J. McLoughlin
Chapter
Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 51)

Abstract

The ecological competence (the ability of microbial cells/inocula to compete and survive in nature) of laboratory/bioreactor prepared inocula is paramount to commercial exploitation of biotechnological processes initiated by the addition of microbial cultures to natural habitats. Such processes include waste-treatment, bioremediation, dairy and food, agricultural and environmental systems and are characterized by a general inability to regulate the process environment stringently. Such inocula systems will require, as a first step, an efficient formulation and delivery system, based on microenvironmental control, directed at minimizing the lag period and maximizing competitive advantage to the introduced microorganisms.

The use of polymer gels, for example alginate, to immobilize cells has allowed the development of spatially organized microenvironments with control on the degree of protection afforded, the rate of cell release and the juxtapositioning of cells with nutrients and/or selective agents or chemicals.

The characteristics of the gel and its shape has a major influence on the microniche created. Through the control of the gellation process the rate of diffusion of nutrients and the rate of polymer breakdown (or cell release) can be regulated. Surface area to volume ratio can influence the biomass distribution as can the initial biomass loading. The radial gradient created (or the resulting degree of nutrient limitation) in gel beads can have significant influence on both the distribution and behaviour of the immobilized biomass. Thus the combination of immobilization technology and nutrient limitation has resulted in the creation of microenvironments in both space and time dimensions. The resultant inocula delivery systems improve the resistance of the culture and regulate the release of cells with enhanced resistance to stress which is advantageous when “the window of opportunity” to ensure successful colonization can be restricted.

Keywords

Immobilize Cell Alginate Bead Calcium Alginate Biomass Distribution Alginate Lyase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Aiden J. McLoughlin
    • 1
  1. 1.Dept. Industrial MicrobiologyUniversity College DublinDublin 4Ireland

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