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Biotechnology Methods pp 47–81Cite as

Extraction and purification of arachidonic acid metabolites from cell cultures

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Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 35))

Abstract

The oxygenated, biologically active, metabolites of arachidonic acid (5,8,11,14-eicosatetraenoic acid), collectively called eicosanoids, include products formed via the cyclooxygenase enzyme complex (prostaglandins, thromboxanes) and via the lipoxygenases (leukotrienes and hydroxy fatty acids). These compounds can be synthesized in vitro by different types of cells in culture but their analysis for metabolic studies or their isolation for subsequent pharmacological studies require proper procedures of extraction and purification. This paper reviews the various techniques of extraction and emphasizes the major and new procedures. In this connection the use of reverse-phase chromatographic material is particularly stressed both for the extraction of the various classes of metabolites by the solid-phase extraction technique and for the purification of classes or individual compounds by high-performance liquid chromatography.

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Abbreviations

AcCOOH:

acetic acid

AcCN:

acetonitrile

AA:

arachidonic acid

DHETE:

dihydroxy-eicosatetraenoic acid

EtOH:

ethanol

EtOAc:

ethylacetate

GC/MS:

gas chromatography/mass spectrometry

HPLC:

high-performance liquid chromatography

HPETE:

hydroperoxy-eicosatetraenoic acid

HETE:

hydroxy-eicosatetraenoic acid

LT:

leukotriene

MeOH:

methanol

ODS:

octadecyl silyl

PMN:

polymorphonuclear leukocytes

PG:

prostaglandin

RBL:

rat basophilic leukemia

RP:

reverse-phase

RIA:

radioimmunoassay

SRS:

slow reacting substance

SP:

straight-phase

TFA:

trifluoroacetic acid

THETE:

trihydroxy-eicosatetraenoic acid

THF:

tetrahydrofuran

TLC:

thin layer chromatography

TX:

thromboxane

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  1. Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Roma, Italy

    C. Bedetti & A. Cantafora

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© 1987 Springer-Verlag

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Bedetti, C., Cantafora, A. (1987). Extraction and purification of arachidonic acid metabolites from cell cultures. In: Biotechnology Methods. Advances in Biochemical Engineering/Biotechnology, vol 35. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0004426

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  • DOI: https://doi.org/10.1007/BFb0004426

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