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Production of L-tryptophan by microbial processes

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Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 26))

Abstract

Recently L-Tryptophan has acquired increasing significance in various fields of the chemical, animal feed and pharmaceutical industries.

For the production of L-tryptophan many kinds of synthetic, enzymatic and fermentation methods are known.

This review places emphasis on microbiological production methods though a brief description of other methods can also be found. After a brief discussion of L-tryptophan biosynthesis and its regulation, the various production methods, the significance of the substituted tryptophans, the main areas of its use and the situation in the tryptophan market will be discussed.

The main purpose of the authors is to review the knowledge of the past twenty years and to summarize this for researchers and industrial fermentation experts. The authors wish to give a widely useful outline of the field, including the less known East European and Soviet results as well. In the authors' opinion, the main question of industrial production of L-tryptophan in the near future will be the economy of production rather than bioengineering research itself.

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Abbreviations

PEP:

phosphoenolpyruvate

E4P:

erythrose 4-phosphate

DAHP:

3-deoxy-D-arabinose-heptulosonic acid 7-phosphate

DHQ:

5-dehydroquinic acid

DHS:

5-dehydroshikimic acid

SA:

shikimic acid

SAP:

shikimic acid 5-phosphate

EPSAP:

3-enolpyruvylshikimic acid 5-phosphate

CA:

chorismic acid

PPA:

prephenic acid

AA:

anthranilic acid

PRA:

N-5′-phosphoribosyl anthranilic acid

CDRP:

1-(o-carboxyphenylamino)-1′-deoxyribulose-5′-phosphate

InGP:

indole-3-glycerol-phosphate

In:

indole

GAP:

glyceraldehid 3-phosphate

Trp:

tryptophan

Phe:

phenylalanine

Tyr:

tyrosine

DAHPS:

3-deoxy-D-arabinose-heptulosonate-7-phosphate synthetase E.C. 4.1.2.15.

DQS:

5-dehydroquinate synthetase

DQ:

5-dehydroquinase E.C. 4.2.1.10.

DSR:

dehydroshikimate reductase E.C. 1.1.1.25.

SK:

shikimate kinase

EPSAPS:

3-enolpyruvylshikimate-5-phosphate synthetase

CS:

chorismate synthetase

CM:

chorismate mutase

AS:

anthranilate synthetase E.C. 4.1.3.27.

PRT:

anthranilate-phosphoribosyl transferase E.C. 2.4.2.18.

PRAI:

N-(5-phosphoribosyl)-anthranilate isomerase

InGPS:

indoleglycerol phosphate synthetase E.C. 4.1.1.48.

TS:

tryptophan synthetase E.C. 4.2.1.20.

P5P:

pyridoxal 5′-phosphate

References

  1. Kinoshita, S.: Adv. Appl. Microbiol. 1, 201 (1959)

    Google Scholar 

  2. Terui, G.: Tryptophan, in Yamada, K. et al.: The Microbial Production of Amino Acids, Kodansha Ltd., Tokyo 1972

    Google Scholar 

  3. Esaki, N. et al.: Biotech. Bioeng. 22, Suppl. 1. 127 (1980)

    Google Scholar 

  4. Hirose, Y., Okada, H.: Microbial Production of Amino Acids, in Microbial Technology, (Peppler, H. J., Perlman, D. ed.) Vol. 1. Academic Press 1979

    Google Scholar 

  5. Hirose, Y., Shibai, H.: Biotech. Bioeng. 22, Suppl. 1. 111 (1980)

    Google Scholar 

  6. Lehninger, A. L.: Biochemistry, Worth Publishers, New York 1972

    Google Scholar 

  7. Margolin, P.: Regulation of Tryptophan Synthesis, in Metabolic Pathways, (Vogel, H. T. ed.) Vol. 5. Metabolic Regulation, Academic Press, New York-London 1971

    Google Scholar 

  8. Truffa-Bachi, P., Cohen, G. N.: Ann. Rev. Biochem. (1973)

    Google Scholar 

  9. Greenberg, D. M.: Carbon Catabolism of Amino Acids, in Greenberg, D. M.: Metabolic Pathways Vol. II. p. 79. Academic Press, New York-London 1961

    Google Scholar 

  10. Miles, E. W., Kumagai, H.: J. Biol. Chem. 249, 2843 (1974)

    Google Scholar 

  11. Zaffaroni, P. et al.: Agr. Biol. Chem. 38, 1335 (1974)

    Google Scholar 

  12. Dinelli, D., Morisi, F. (Snam Progetti SPA): German Pat. 2225797

    Google Scholar 

  13. Colowick, S. P., Kaplan, N. O.: Methods in Enzymology, Vol. II. Academic Press, New York 1955

    Google Scholar 

  14. Aurich, M., Schöpp, G.: Z. Allg. Mikrobiologie 13, 545 (1973)

    Google Scholar 

  15. Matchett, W. H.: J. Biol. Chem. 249, 4041 (1974)

    Google Scholar 

  16. Abbott, B. J.: Adv. Appl. Microbiol. 20, 232 (1977)

    Google Scholar 

  17. Hitoshi, E. et al. (Ajinomoto): US Pat. 3808101

    Google Scholar 

  18. Sano, K., Mitsugi, K. (Ajinomoto): German Pat. 2461188

    Google Scholar 

  19. Teuscher, G., Teuscher, E.: Acta Biol. Med. German 19, 211 (1967)

    Google Scholar 

  20. Watanabe, T., Snell, E. E.: Proc. Nat. Acad. Sci. USA 69, 1086 (1972)

    Google Scholar 

  21. Yamada, M.: J. Ferment. Technol. 32, 259 (1974)

    Google Scholar 

  22. Lester, G.: J. Bact. 107, 448 (1971)

    Google Scholar 

  23. Shetty, A. S., Gaertner, F. M.: J. Bact. 113, 1127 (1973)

    Google Scholar 

  24. Isono, K., Mino, Y.: J. Japan Grassl. Sci. 16, 285 (1970)

    Google Scholar 

  25. Elander, R. P. et al.: Appl. Microbiol. 16, 753 (1968)

    Google Scholar 

  26. Teuscher, G.: Allg. Mikrobiol. 7, 393 (1967)

    Google Scholar 

  27. Teuscher, G.: Allg. Mikrobiol. 11, 431 (1971)

    Google Scholar 

  28. Doy, C. M.: Rev. Pure Appl. Chem. 18, 41 (1968)

    Google Scholar 

  29. Meuris, P.: Bull. Soc. Chim. Biol. 49, 1573 (1967)

    Google Scholar 

  30. Jensen, R. A. et al.: J. Bacteriol. 94, 1582 (1967)

    Google Scholar 

  31. Gibson, F., Pittard, J.: Bacteriol. Rev. 32, 465 (1968)

    Google Scholar 

  32. Doy, C. M.: Biochem. Biophys. Res. Commun. 26, 187 (1967)

    Google Scholar 

  33. Pittard, J. et al.: J. Bacteriol. 97, 1242 (1969)

    Google Scholar 

  34. Hütter, R., DeMoss, J. A.: J. Bacteriol. 94, 1896 (1967)

    Google Scholar 

  35. Carlton, B. C.: J. Bacteriol. 94, 660 (1967)

    Google Scholar 

  36. Kida, S. et al.: J. Ferment. Technol. 43, 233 (1965)

    Google Scholar 

  37. Maurer, R., Crawford, I. P.: J. Bacteriol. 106, 331 (1971)

    Google Scholar 

  38. Miozzari, G. et al.: J. Bacteriol. 134, 48 (1978)

    Google Scholar 

  39. Baskerville, E. N.: J. Bacteriol. 110, 146 (1972)

    Google Scholar 

  40. Carsiotis, M., Jones, R. F.: J. Bacteriol. 119, 889 (1974)

    Google Scholar 

  41. Lester, G.: J. Bacteriol. 107, 193 (1971)

    Google Scholar 

  42. Miozzari, G. et al.: Arch. Microbiol. 115, 307 (1977)

    Google Scholar 

  43. Schürch, A. et al.: J. Bacteriol. 117, 131 (1974)

    Google Scholar 

  44. Petrov, D. F. et al.: USSR Pat. 310930

    Google Scholar 

  45. Petrov, D. F. et al.: USSR Pat. 293845

    Google Scholar 

  46. Kaneo, T., Izumi, Y.: Synthetic Production of Amino Acids, Kodansha Ltd., Tokyo 1974

    Google Scholar 

  47. Sano, K. et al.: Agr. Biol. Chem. 41, 819 (1977)

    Google Scholar 

  48. Ishii, K., Takanami, K.: Japan Pat. 7886093

    Google Scholar 

  49. Okazaki, H.: Agr. Biol. Chem. 32, 254 (1968)

    Google Scholar 

  50. Konosuke, S., Mitsugi, K.: US Pat. 3929573

    Google Scholar 

  51. Lim, P. G., Mateles, R. I.: J. Bacteriol. 87, 1051 (1964)

    Google Scholar 

  52. Bang, W. G. et al.: Prep. Eur. Cong. Biotechnol. 1st 1978, 186-9 Dechema

    Google Scholar 

  53. Decottignies-Le Marechal, P. et al.: Eur. J. Appl. Microbiol. Biotechnol. 7, 33 (1979)

    Google Scholar 

  54. Sahm, H., Zähner, H.: Arch. Microbiol. 76, 223 (1971)

    Google Scholar 

  55. Hoch, S. O. et al.: J. Bact. 105, 38 (1971)

    Google Scholar 

  56. Prozorov, A. A. et al.: USSR Pat. 408758, 324860

    Google Scholar 

  57. Shiio, I. et al.: Agr. Biol. Chem. 37, 1991 (1973)

    Google Scholar 

  58. Thiemann, J. E., Pagani, H.: British Pat. 1298499, USA Pat. 3700558, USSR Pat. 351375, German Pat. 2037763

    Google Scholar 

  59. Lim, P. G., Mateles, R. I.: Science 140, 188 (1963)

    Google Scholar 

  60. Bandiera, M. et al.: Experientia 23, 724 (1967)

    Google Scholar 

  61. Shiio, I. et al.: Agr. Biol. Chem. 39, 627 (1975)

    Google Scholar 

  62. Nakayama, K., Hagino, M. (Kyowa Hakko Kogyo Co.): German Pat. 1903336

    Google Scholar 

  63. Nakayama, K. et al. (Kyowa Hakko Kogyo Co.): Japan Pat. 50-135284

    Google Scholar 

  64. Nakayama, K. et al. (Kyowa Hakko Kogyo Co.): German Pat. 2357100

    Google Scholar 

  65. Hagino, M., Nakayama, K.: Agr. Biol. Chem. 39, 343 (1975)

    Google Scholar 

  66. Suzuki, M. et al.: J. Ferment. Technol. 55, 466 (1977)

    Google Scholar 

  67. Kida, S., Matsushiro, A.: J. Ferment. Technol. 43, 302 (1965)

    Google Scholar 

  68. Kida, S., Matsushiro, A.: J. Ferment. Technol. 43, 307 (1965)

    Google Scholar 

  69. Maksimova, E. A. et al.: Khim. Farm. Zh. 11, 75 (1977)

    Google Scholar 

  70. Semenova, L. E. et al.: Khim. Farm. Zh. 12, 94 (1978)

    Google Scholar 

  71. Moran, F., Raboin, D.: Proc. IV. IFS: Ferment. Technol. Today 429 (1972)

    Google Scholar 

  72. Shirai, K. et al.: Japan Pat. 77125695

    Google Scholar 

  73. Shiio, I., Sugimoto, S.: J. Biochem. (Tokyo) 83, 879 (1978)

    Google Scholar 

  74. Malin, B.: USA Pat. 2999051

    Google Scholar 

  75. Malin, B., Westhead, J.: J. Biochem. Microbiol. Techn. Eng. 1, 49 (1959)

    Google Scholar 

  76. Arima, K. et al.: USA Pat. 3801457, French Pat. 2128669

    Google Scholar 

  77. Vyskumny Ustav Antibiotik a Biotransformaci: Czechoslovak Pat. PV 7345-78

    Google Scholar 

  78. Vyskumny Ustav Antibiotik a Biotransformaci: Czechoslovak Pat. PV 7392-78

    Google Scholar 

  79. Sanchez, S., Demain, A. L.: Appl. Environ. Microbiol. 35, 459 (1978)

    Google Scholar 

  80. Aktiebolaget Bofors: French Pat. 2263302

    Google Scholar 

  81. Kyowa Hakko Kogyo Co.: French Pat. 2568652

    Google Scholar 

  82. Kareva, I. I. et al.: Prikl. Biochim. Mikrobiol. 2, 625 (1966)

    Google Scholar 

  83. Gribiny, P. P.: Poluchenie i primenenie aminokislot, Riga, 1970

    Google Scholar 

  84. Terui, G. et al.: J. Ferment. Technol. 40, 120 (1962)

    Google Scholar 

  85. Enatsu, T., Terui, G.: USA Pat. 3296090

    Google Scholar 

  86. Enatsu, T. et al.: J. Ferment. Technol. 41, 500 (1963)

    Google Scholar 

  87. Terui, G. et al.: J. Ferment. Technol. 40, 441 (1962)

    Google Scholar 

  88. Paulsson, L. E. et al., (Bofors Ab): Swedish Pat. 1231/1972

    Google Scholar 

  89. Sevella, B. et al.: L-Tryptophan Production by Fermentation. Paper Presented at VI. Int. Ferm. Symp. 1980. London, Canada, July 20–25, 1980

    Google Scholar 

  90. Pécs, M. et al.: L-Tryptophan Production by Fermentation. Paper presented at 2. Symp. der Sozialistischen Länder über Biotechn. Leipzig 2–5. 12. 1980

    Google Scholar 

  91. Sevella, B. et al.: L-Tryptophan. Paper presented at Hung. Ann. Meet. Biochem. Siófok, Hungary 1–3. 10. 1980

    Google Scholar 

  92. Pécs, M.: Tryptophan Fermentation. Ph. D. Dissertation, Univ. Of Techn. Sciences, Budapest 1980

    Google Scholar 

  93. Aboliny, T. K.: Fermentacija 53 (1974)

    Google Scholar 

  94. Ruban, E. L., Ozoliny, R. K.: Aminokisloti mikrobnogo sinteza, Riga 1968

    Google Scholar 

  95. Enatsu, T. et al.: J. Ferment. Technol. 41, 61 (1963)

    Google Scholar 

  96. Terui, G., Enatsu, T.: J. Ferment. Technol. 40, 252 (1962)

    Google Scholar 

  97. Niitsu, M. et al.: J. Ferment. Technol. 47, 194 (1969)

    Google Scholar 

  98. Aboliny, T. K. et al.: Prikl. Biohim. Mikrobiol. 6, 633 (1970)

    Google Scholar 

  99. Beker, M. E. et al.: Prikl. Biohim. Mikrobiol. 7, 103 (1971)

    Google Scholar 

  100. Ruban, E. L. et al.: USSR Pat. 299 542

    Google Scholar 

  101. Ruban, E. L. et al.: USSR Pat. 247315

    Google Scholar 

  102. Viesztur, U. E. et al.: USSR Pat. 353955

    Google Scholar 

  103. Abolins, T., Helmanis, R.: Upr. Mikrobn. Sintezom. 48 (1977)

    Google Scholar 

  104. Abolins, T. et al.: Technologija Mikrobnogo Sinteza 52 (1978)

    Google Scholar 

  105. Kida, S. et al.: J. Ferment. Technol. 49, 390 (1971)

    Google Scholar 

  106. Ebihara, Y. et al.: J. Ferment. Technol. 47, 733 (1969)

    Google Scholar 

  107. Kyowa Hakko Kogyo Co.: British Pat. 1222904, German Pat. 1948796

    Google Scholar 

  108. Eldib Engineering and Research Inc.: Chemical Market Research Survey on Tryptophan, 1977

    Google Scholar 

  109. Eldib Engineering and Research Inc. Summit (N.): Chem. Mark. Rep. 211, 22 (1977)

    Google Scholar 

  110. Fukuda, D. S. et al.: Appl. Microbiol. 21, 841 (1971)

    Google Scholar 

  111. Partridge, S. M.: Biochem. J. 45, 459 (1949)

    Google Scholar 

  112. Partridge, S. M., Brimley, R. C.: Biochem. J. 44, 513 (1949)

    Google Scholar 

  113. Raminya, L. O. et al.: Fermentacija 133 (1973)

    Google Scholar 

  114. Kyowa Hakko Kogyo Co.: French Pat. 2000632

    Google Scholar 

  115. Aboliny, T. K. et al.: Fermentacija 124 (1973)

    Google Scholar 

  116. Tribe, D. E., Pittard, J.: Appl. Environ. Microbiol. 38, 181 (1979)

    Google Scholar 

  117. Applezweig, N.: Business Week Dec. 14 (1981)

    Google Scholar 

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Authors and Affiliations

  1. University of Technical Sciences, Budapest

    László Nyeste, Miklós Pécs, Béla Sevella & János Holló

  2. Institute of Agricultural Chemical Technology, Gellért tér 4, 1521, Budapest, Hungary

    László Nyeste, Miklós Pécs, Béla Sevella & János Holló

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  1. László Nyeste
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  2. Miklós Pécs
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  3. Béla Sevella
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  4. János Holló
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Nyeste, L., Pécs, M., Sevella, B., Holló, J. (1983). Production of L-tryptophan by microbial processes. In: Downstream Processing. Advances in Biochemical Engineering/Biotechnology, vol 26. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0001863

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  • DOI: https://doi.org/10.1007/BFb0001863

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  • Print ISBN: 978-3-540-12096-4

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