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Production of L-tryptophan by microbial processes

Conference paper
Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 26)

Abstract

Recently L-Tryptophan has acquired increasing significance in various fields of the chemical, animal feed and pharmaceutical industries.

For the production of L-tryptophan many kinds of synthetic, enzymatic and fermentation methods are known.

This review places emphasis on microbiological production methods though a brief description of other methods can also be found. After a brief discussion of L-tryptophan biosynthesis and its regulation, the various production methods, the significance of the substituted tryptophans, the main areas of its use and the situation in the tryptophan market will be discussed.

The main purpose of the authors is to review the knowledge of the past twenty years and to summarize this for researchers and industrial fermentation experts. The authors wish to give a widely useful outline of the field, including the less known East European and Soviet results as well. In the authors' opinion, the main question of industrial production of L-tryptophan in the near future will be the economy of production rather than bioengineering research itself.

Keywords

Aromatic Amino Acid Microbial Process Neurospora Crassa Shikimic Acid Anthranilic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

List of Abbreviations

PEP

phosphoenolpyruvate

E4P

erythrose 4-phosphate

DAHP

3-deoxy-D-arabinose-heptulosonic acid 7-phosphate

DHQ

5-dehydroquinic acid

DHS

5-dehydroshikimic acid

SA

shikimic acid

SAP

shikimic acid 5-phosphate

EPSAP

3-enolpyruvylshikimic acid 5-phosphate

CA

chorismic acid

PPA

prephenic acid

AA

anthranilic acid

PRA

N-5′-phosphoribosyl anthranilic acid

CDRP

1-(o-carboxyphenylamino)-1′-deoxyribulose-5′-phosphate

InGP

indole-3-glycerol-phosphate

In

indole

GAP

glyceraldehid 3-phosphate

Trp

tryptophan

Phe

phenylalanine

Tyr

tyrosine

DAHPS

3-deoxy-D-arabinose-heptulosonate-7-phosphate synthetase E.C. 4.1.2.15.

DQS

5-dehydroquinate synthetase

DQ

5-dehydroquinase E.C. 4.2.1.10.

DSR

dehydroshikimate reductase E.C. 1.1.1.25.

SK

shikimate kinase

EPSAPS

3-enolpyruvylshikimate-5-phosphate synthetase

CS

chorismate synthetase

CM

chorismate mutase

AS

anthranilate synthetase E.C. 4.1.3.27.

PRT

anthranilate-phosphoribosyl transferase E.C. 2.4.2.18.

PRAI

N-(5-phosphoribosyl)-anthranilate isomerase

InGPS

indoleglycerol phosphate synthetase E.C. 4.1.1.48.

TS

tryptophan synthetase E.C. 4.2.1.20.

P5P

pyridoxal 5′-phosphate

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  1. 1.University of Technical SciencesBudapest
  2. 2.Institute of Agricultural Chemical TechnologyBudapestHungary

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