Abstract
The application of ultrafiltration in biotechnology is reviewed emphasizing the separation of catalytically active species. Ultrafiltration as a separation process as well as its application to membrane reactors is analyzed. On the basis of an application-oriented theory of ultrafiltration, the essential aspects for process design are described. A survey of applications is given.
Ultrafiltration has proved to be a very versatile separation process for biocatalysts owing to the possibility of independently adjusting the temperature, the avoidance of phase transition and the low energy requirement. It will be shown that ultrafiltration devices, suitable for the isolation of biocatalysts, can also be applied efficiently for their re-use in catalytic processes. The main advantages of employing biocatalysts in ultrafiltration membrane reactors are that continuous operation is possible in homogeneous phase and that immobilization know-how is not required.
Future trends can be predicted with respect to the development of sterilisable membranes with improved narrow pore size distribution and with surfaces that will not affect fragile biocatalysts. Continuous coenzyme regeneration in membrane reactors and biomass recycling in continuous fermentation processes, in order to uncouple the retention time of the catalyst from the hydraulic retention time will result in increased application.
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Abbreviations
- a m2 m−3 :
-
volume specific area
- A m2 :
-
area
- c mol m−3 :
-
concentration
- D m2 s−1 :
-
diffusion coefficient
- d m:
-
diameter
- E kg m−3 :
-
enzyme concentration
- Ea J mol−1 :
-
energy of activation
- f:
-
ratio
- J m s−1 :
-
flux (area specific volumetric flow rate)
- Ji mol m−2 s−1 :
-
molar flux of substance i
- k2 mol kg−1 s−1 :
-
reaction rate constant
- kd m s−1 :
-
mass-transfer coefficient
- kde s−1 :
-
deactivation rate constant
- Kic mol m−3 :
-
constant for competitive product inhibition
- Km mol m−3 :
-
Michaelis-Menten constant
- l m:
-
length
- m kg:
-
mass
- M kg kmol−1 :
-
molar mass
- n:
-
number of stage(s)
- p Pa=N m−2 :
-
pressure
- r m:
-
radius
- rm mol kg−1 s−1 :
-
catalyst mass specific reaction rate
- ¯rm mol kg−1 s−1 :
-
catalyst mass specific productivity
- rv mol m−3 s−1 :
-
reactor volume specific reaction rate
- R:
-
apparent retention
- Rm :
-
intrinsic retention
- S mol m−3 :
-
substrate concentration
- t s:
-
time
- ¯t s:
-
mean residence time
- u m s−1 :
-
linear velocity
- V m3 :
-
volume
- V m3 s−1 :
-
volumetric flow rate
- W Pa s m−1 :
-
hydraulic resistance
- X:
-
substrate conversion
- x m:
-
local coordinate
- γ s−1 :
-
shear rate (absolute value)
- δ m:
-
laminar boundary layer thickness
- ε:
-
porosity
- η Pa s:
-
dynamic viscosity
- ν m2 s−1 :
-
kinematic viscosity
- π Pa:
-
osmotic pressure
- ϱ kg m−3 :
-
density
- σ 2p m2 :
-
variance of pore diameter
- σ 2i m2 :
-
variance of particle diameter
- τ Pa:
-
shear stress
- τm kg s m−3 :
-
catalyst mass referred space time
- θ:
-
dimensionless time (t/¯t)
- b:
-
bulk phase
- c:
-
convective
- d:
-
diffusive — (excl. kd)
- e:
-
end-, final
- E:
-
enzyme
- f:
-
filtrate
- g:
-
gel
- h:
-
hydraulic
- i:
-
referred to species i
- m:
-
membrane- (excl. τm rm, Km)
- n:
-
referred to stage n, number of stages
- o:
-
initial-, feed
- p:
-
pore
- P:
-
productivity referred
- r:
-
radial
- R:
-
reactor
- s:
-
saturation
- S:
-
separation unit
- t:
-
tube
- V:
-
volume
- w:
-
water (eluant)
- max:
-
maximum
- opt:
-
optimum
- rec:
-
recycle
- Σ:
-
total
- ADH:
-
alcohol dehydrogenase
- A(D)TP:
-
adenosine (di)triphosphate
- AlaDH:
-
alanine dehydrogenase
- CMR:
-
cascade of completely equipped UFMR
- CRMR:
-
cascade recycle membrane reactor
- CSTR:
-
continuous stirred tank reactor (ideal)
- FDH:
-
formate dehydrogenase
- LDH:
-
lactate dehydrogenase
- LeuDH:
-
leucine dehydrogenase
- MR:
-
membrane reactor (general)
- PEG:
-
polyethylene glycol
- PFTR:
-
plug flow tubular reactor
- PVA:
-
polyvinyl alcohol
- PWTR:
-
porous wall tubular reactor
- SBR:
-
stirred batch reactor
- TRMR:
-
tubular recycle membrane reactor
- UFMR:
-
continuously operated single stage ultrafiltration membrane reactorx
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Flaschel, E., Wandrey, C., Kula, MR. (1983). Ultrafiltration for the separation of biocatalysts. In: Downstream Processing. Advances in Biochemical Engineering/Biotechnology, vol 26. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0001861
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DOI: https://doi.org/10.1007/BFb0001861
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