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The formation of protein precipitates and their centrifugal recovery

Conference paper
Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 26)

Abstract

The methods of precipitating proteins by the addition of reagents are outlined from a physicochemical viewpoint prior to a more detailed consideration of the molecular and colloidal processes by which precipitation occurs. The mixing necessary for efficient contacting of the reagent and protein and for development of the precipitate also generates forces which can break down precipitate particles. The factors which influence the balance between these opposing processes are analysed as one essential element in reactor design. Other elements, including the influence of reactor configuration, are dealt with and the effects of ageing and other precipitate conditioning methods are described. After outlining the options available for precipitate recovery, centrifugal separation is examined in detail and related to the factors which have been shown to influence precipitate characteristics. Several typical industrial precipitation and recovery operations are described and the key factors influencing process design are summarized.

Keywords

Soya Protein Ammonium Sulphate Protein Precipitate Shear Field Isoelectric Precipitation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Nomenclature

C

concentration, kg m−3

d, dx

particle diameter, where x refers to the wt% oversize value, m

Ds

dielectric constant

D

diffusivity, m2 s−1

fij

interaction coefficient, Eq. (5)

¯G

mean velocity gradient, s−1

G

shear rate, s−1

G(d)

gravimetric grade efficiency function

h

centrifuge disc separation distance, m

I

ionic strength, kmol m−3

Is

intensity of segregation, Eq. (31)

kD

constant in density Eq. (36)

k

Boltzmann constant, 1.3805×10−23 JK−1

KA

rate constant, Eq. (9), m3 s−1

K

constant in solubility equations

N

particle number concentration, m−3

Ns

stirrer speed, rps.

Q

centrifuge feed rate, m3 s−1

q

net surface charge on a molecule, C

r

radial position in centrifugal field, m

R

gas constant, 8.314 Jmol−1 K−1

Rep

particle Reynolds number=ϱfdv/μ

S

solubility, kg m−3

t

time, s

T

absolute temperature, K

v

velocity, m s−1

Greek symbols

α

collision effectiveness factor, Eq. (17)

β, β′

constants in solubility equations

Δϱ

density difference: ϱa−ϱf, kg m−3

ε

energy dissipated per unit mass, W kg−1

η

turbulent microscale, m

θ

included angle between centrifuge axis and disc surface

μi

chemical potential of species i, Jmol−1

μ

dynamic viscosity, Ns m−2

ν

kinematic viscosity, m2 s−1

ϱa

aggregate density, kg m−3

ϱf

fluid density, kg m−3

ϕ(h)

potential energy of interaction between two particles at distance h apart, J

ϕv

volume fraction of particles in suspension

ω

angular velocity, rad s−1

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  1. 1.Department of Chemical and Biochemical EngineeringUniversity College LondonLondonUK

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