Abstract
By simulating the functions of the animal intestine, the authors have developed a novel tubular bioreactor (TBR) which is capable of containing both the reaction and separation of products in a single system. This reactor consisted of inorganic ultra filtration membrane modules in the primary part of the system, a heat exchanger and a recycling pump.
The operation characteristics of the TBR were studied by cultivating Lactobacillus casei at a laboratory scale. The cell density obtained was up to 10 times higher than the density obtained by using the conventional jar fermentor. Furthermore, 40 g l−1 of cell mass was obtained in only 14 hours with 20 l of fresh medium when the dilution rate was increased according to the cellular growth. Afterwards, the cultivation time and the volume of fresh medium were reduced to 44% and 74%, respectively, of the values in the cultivation operation at constant dilution rate.
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Abbreviations
- D:
-
dilution rate (h−1)
- F:
-
feed rate of fresh medium (g l−1)
- P:
-
lactic acid concentration (g l−1)
- S:
-
glucose concentration in the reactor (g l−1)
- Sf :
-
glucose concentration in the feed of fresh medium (g l−1)
- t:
-
time (h)
- X:
-
cell mass concentration (g dry cell l−1)
- Μ:
-
specific growth rate (h−1)
- Ν:
-
specific glucose consumption rate (h−1)
- π:
-
specific lactic acid production rate (h−1)
References
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© 1990 Springer-Verlag
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Endo, I., Nagamune, T., Tachikawa, S., Inaba, H. (1990). A tubular bioreactor for high density cultivation of microorganisms. In: Bioprocesses and Applied Enzymology. Advances in Biochemical Engineering/Biotechnology, vol 42. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0000728
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DOI: https://doi.org/10.1007/BFb0000728
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Publisher Name: Springer, Berlin, Heidelberg
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Online ISBN: 978-3-540-47150-9
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