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Growth limitations in microcarrier cultures

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Vertrebrate Cell Culture I

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 34))

Abstract

The use of various commercially avaiable microcarriers is considered for the large-scale growth of anchorage-dependent animal cells. Cell productivity in such cultures can be maximised by an analysis of various kinetic aspects of growth. Such parameters as the critical cell to bead ratio at inoculation is particularly important in this respect.

The full theoretical capacity of microcarriers to support cell growth can only be realised by an understanding of the interaction of the cell with is micro-environment. The final cell yield in culture may be limited by the supply of nutrients or by the accumulation of inhibitory metabolites. Glucose and glutamine are widely used as the major energy sources for cell growth and their relative concentrations in the media can influence cellular metabolism. The products of such metabolism — lactic acid and ammonia — may be inhibitory and methods to reduce their accumulation should be considered. The requirements for other essential metabolites such as amino acids vary but their supply should reflect the needs of each specific cell type. The advantages of serum-free media and the problems of oxygen supply particularly in large-scale microcarrier cultures are reviewed.

Full investigation of these parameters that affect cell productivity will result in an increased ability to control the culture of anchorage-dependent animal cells on microcarriers. This will lead to considerable benefits for the production of an expanding list of useful cell products.

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Abbreviations

ADP:

adenosine diphosphate

ATP:

adenosine triphosphate

cAMP:

cyclic adenosine monophosphate

DEAE:

diethyl amino ethane

DMAP:

dimethyl amino propane

DOT:

dissolved oxygen tension

EDTA:

ethylene diamine tetra-acetic acid

LH:

lactalbumin hydrolysate

MDCK:

Madin Darby canine kidney

OTR:

oxygen transfer rate

OUR:

oxygen utilisation rate

PBS:

phosphate buffered saline

TCA:

tricarboxylic acid

TPB:

tryptose phosphate broth

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  1. Department of Biological Sciences, Manchester Polytechnic, Chester Street, MI5GD, Manchester, UK

    M. Butler

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© 1987 Springer-Verlag

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Butler, M. (1987). Growth limitations in microcarrier cultures. In: Vertrebrate Cell Culture I. Advances in Biochemical Engineering/Biotechnology, vol 34. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0000673

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  • DOI: https://doi.org/10.1007/BFb0000673

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