Skip to main content
SpringerLink
Log in

Nerve and muscle cells on microcarriers in culture

  • Conference paper
  • First Online:
Vertrebrate Cell Culture I

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 34))

Abstract

Commercially available microcarriers (MCs), which are briefly described here, are usually used as a support for culturing a variety of cells both on laboratory and on an industrial scale. Selected MCs are used as a culture system for embryonic dissociated CNS cells and myoblasts. The tridimensional support provided by the MCs, enables neuronal and muscle cells to grow and differentiate into functional cell-MC units, which remain floating in the culture medium. The neuronal entities are characterized by intensive fiber growth followed by synaptogenesis and myelination. The muscular units develop striated myotubes, having the same orientation, which contract spontaneously. The MC technique is advantageous over the conventional monolayer procedure, allowing cells to grow to higher amounts at a better efficiency for longer periods. Functional units can be sampled without interfering with the ongoing culture. We consider the use of these cultures, as well as nerve muscle MC co-cultures as a tool for the study of neurotoxicology. The possibility of implantation of such functional culture units into injured adult nerve and muscle tissues is being considered.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Abbreviations

AChE:

Acetylcholinesterase

CNS:

Central nervous system

DIV:

Days in vitro

MC:

Microcarrier

MCs:

Microcarriers

SEM:

Scanning electron microscope or micrographs

TEM:

Transmission electron microscope or micrographs

References

  1. Reuveny, A.: Microcarriers for culturing mammalian cells and their application, in: Advances in Biotechnological Processes (eds. Mizrahi, A., Van Wezel, A. L.) Vol. 2, p. 1, New York, Alan R. Liss 1983

    Google Scholar 

  2. Davis, P. F.: Exp. Cell Res. 134, 367 (1981)

    Google Scholar 

  3. Ryan, U. S., Mortara, M., Whitaker, C.: Tissue Cell 12, 619 (1980)

    Google Scholar 

  4. Horst, J., Kern, M., Ulmer, E.: Eur. J. Cell Biol. 22, 599 (1980)

    Google Scholar 

  5. Duda, E.: Cryo. Lett. 3, 67 (1982)

    Google Scholar 

  6. Shahar, A., Reuveny, S., Amir, A., Kotler, M., Mizrahi, A.: J. Neurosci. Res. 9, 339 (1983)

    Google Scholar 

  7. Sargent, G. F., Sims, T. A., McNeish, A. S.: J. Microsc. 122, 209 (1981)

    Google Scholar 

  8. Crespi, C. L., Thilly, W. G.: Mutation Res. 106, 123 (1982)

    Google Scholar 

  9. Wright, J. T., Elmer, W. A., Dunlop, A. T.: Anal. Lab. 125, 100 (1982)

    Google Scholar 

  10. Lai, C. S., Hopwood, L. E., Swartz, H. H.: Exp. Cell Res. 130, 437 (1980)

    Google Scholar 

  11. Hoeg, J. M., Osborne, J. C., Brewer, H. B.: J. Biol. Chem. 257, 2125 (1982)

    Google Scholar 

  12. Vosbeck, K., Roth, S.: J. Cell Sci. 22, 657 (1976)

    Google Scholar 

  13. Fairman, K., Jacobson, B. S.: Tissue Cell 15, 167 (1983)

    Google Scholar 

  14. Kotler, M., Reuveny, S., Mizrahi, A., Shahar, A.: Develop. Biol. Stand. 60, 255 (1985)

    Google Scholar 

  15. Davis, P. F., Kerr, C.: Exp. Cell Res. 141, 455 (1982)

    Google Scholar 

  16. Davis, P. F., Ganz, P., Diehl, P. S.: Lab. Invest. 85, 710 (1985)

    Google Scholar 

  17. Ren, E. C.: J. Immunol. Meth. 49, 105 (1982)

    Google Scholar 

  18. Howard, G. A., Turner, R. T., Fuzas, J. E., Nichols, F., Baylink, D. J.: JAMA 249, 258 (1983)

    Google Scholar 

  19. Fayet, B., Hovsepian, S.: Biochimie 61, 923 (1979)

    Google Scholar 

  20. Smith, M. A., Vale, W. W.: Endocrinology 107, 1425 (1980)

    Google Scholar 

  21. Smith, M. A., Vale, W. W.: ibid. 108, 752 (1981)

    Google Scholar 

  22. Spiess, Y., Smith, M. A., Vale, W.: Diabetes 31, 189 (1982)

    Google Scholar 

  23. Busch, C., Cancilia, P. A., DeBault, L. E., Goldsmith, J. C., Owen, W. G.: Lab. Invest. 47, 498 (1982)

    Google Scholar 

  24. Shainberg, A., Isac, A., Reuveny, S., Mizrahi, A., Shahar, A.: Cell Biol. Int. Rep. 7, 727 (1983)

    Google Scholar 

  25. Pawlowski, R., Szigeti, V., Loyd, R., Pryzbylski, R. J.: Eur. J. Cell Biol. 32, 296 (1983)

    Google Scholar 

  26. Ostlund, C., Clark, J., Kruse, M.: In Vitro 19, 279 (1983)

    Google Scholar 

  27. Bone, A. J., Swenne, I.: ibid. 18, 141 (1982)

    Google Scholar 

  28. Reuveny, S., Mizrahi, A., Shahar, A., Kotler, M.: Cell Biol. Int. Rep. 8, 7, 539 (1984)

    Google Scholar 

  29. Shahar, A., Amir, A., Reuveny, S., Silberstein, L., Mizrahi, A.: Develop. Biol. Standard 55, 25 (1984)

    Google Scholar 

  30. Shahar, A., Mizrahi, A., Reuveny, S., Zinman, T., Shainberg, A.: ibid. 60, 263 (1985)

    Google Scholar 

  31. Shahar, A., Reuveny, S., Mizrahi, A., Shainberg, A.: J. Acad. Med. Torino CXLVII, 33 (1984)

    Google Scholar 

  32. Reuveny, S., Silberstein, L., Shahar, A., Freeman, E., Mizrahi, A.: In Vitro 18, 92 (1982a)

    Google Scholar 

  33. Reuveny, S., Silberstein, L., Shahar, A., Freeman, E., Mizrahi, A.: Develop. Biol. Stand. 50, 115 (1982b)

    Google Scholar 

  34. Hirtenstein, M., Clark, J., Lindgren, G., Vertblad, P.: ibid. 46, 109 (1980)

    Google Scholar 

  35. Lewis, D. H., Volkers, S. A. S.: ibid. 42, 147 (1979)

    Google Scholar 

  36. Gebb, C., Clark, J. M., Hirtenstein, M. D., Lindgren, G., Lindgren, U., Lindskog, U., Lundgren, B., Vertblad, P.: ibid. 50, 93 (1982)

    Google Scholar 

  37. Paris, M. S., Eaton, D. L., Sempolinski, D. E., Sharma, B. P.: In vitro 19, 262 (1983)

    Google Scholar 

  38. Johansson, A., Nielsen, V.: Develop. Biol. Stand. 46, 125 (1980)

    Google Scholar 

  39. Nielsen, V., Johansson, A.: ibid. 46, 131 (1980)

    Google Scholar 

  40. Varani, J., Dame, M., Beals, T. F., Wass, J. A.: Biotech. Bioeng. 25, 1359 (1983)

    Google Scholar 

  41. Varani, J., Dame, M., Rediske, J., Beals, T. F., Hellegas, W.: J. Biol. Stand. 13, 67 (1985)

    Google Scholar 

  42. Gahwiler, B. H.: Neuroscience 11, 751 (1984)

    Google Scholar 

  43. Sobkowicz, A. M., Bleier, R., Bereman, B.: J. Neurocytol. 3, 431 (1974)

    Google Scholar 

  44. Gahwiler, B. H.: Experientia 40, 235 (1984)

    Google Scholar 

  45. Yavin, Z., Yavin, E.: Exp. Brain Res. 29, 137 (1977)

    Google Scholar 

  46. Shahar, A. et al.: Aging in cerebral and motor neurons of fetal and adult origin, in: The Aging Brain: Cellular and Molecular Mechanisms of Aging in the Nervous System (eds. Giacobini, E. et al.) p. 35, New York, Raven Press 1982

    Google Scholar 

  47. Sensenbrenner, M.: Proliferation and maturation of neuronal cells from the central nervous system in culture, in: Neurotransmitter Interaction and Compartmentation (ed. Bradford, H. F.) p. 497, Plenum Publishing Corporation 1982

    Google Scholar 

  48. Sensenbrenner, M.: Dissociated brain cells in primary cultures, in: Cell, Tissue and Organ Cultures in Neurobiology (eds. Fedoroff, S., Hertz, L.) p. 191 New York, Academic Press 1977

    Google Scholar 

  49. Seeds, N. W., Ramirez, G., Marko, M. J.: Aggregate cultures: A model for studies of brain development, in: Cell Culture and its Application (eds. Acton, R. T., Lynn, J. D.), New York, Academic Press 1977

    Google Scholar 

  50. Honegger, P.: Brain Res. 162, 89 (1979)

    Google Scholar 

  51. Matthieu, J. M., Honegger, P., Favrod, P., Poduslo, J. F., Ceccarini, C., Kristic, R.: Myelination and demyelination in aggregating cultures of rat brain cells, in: Tissue Culture in Neurobiology (eds. Giacobini, E., Vernadakis, A., Shahar, A.), New York, Raven Press 1980

    Google Scholar 

  52. Matthieu, J. M., Honegger, P., Trapp, B. D., Cohen, S. R., Webster, H. De.: Neuroscience 3, 565 (1978)

    Google Scholar 

  53. Seeds, N. W., Haffke, S. C.: Dev. Neurosci. 1, 69 (1978)

    Google Scholar 

  54. Johnson, C. D., Russel, R. L.: Anal. Biochem. 64, 229 (1975)

    Google Scholar 

  55. Yaffe, D.: Current Topics in Develop. Biol. 4, 37 (1969)

    Google Scholar 

  56. Shainberg, A., Yagil, G., Yaffe, D.: Develop. Biol. 25, 1 (1971)

    Google Scholar 

  57. Shainberg, A.: Nature 264, 368 (1976)

    Google Scholar 

  58. Fambrough, D. M.: Physiol. Rev. 59, 165 (1979)

    Google Scholar 

  59. Koenig, J.: Prog. Brain Res. 49, 484 (1979)

    Google Scholar 

  60. Shainberg, A., Shahar, A., Burstein, M., Giacobini, E.: Effect of innervation on acetylcholine receptors in muscle cultures, in: Tissue Culture in Neurobiology (eds. Giacobini, E. et al.) p. 25, New York, Raven Press 1980

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Section of Electron Microscopy, Department of Biotechnology, Israel Institute for Biological Research, P.O. Box 19, 70450, Ness-Ziona, Israel

    A. Shahar & S. Reuveny

Authors
  1. A. Shahar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Reuveny
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 1987 Springer-Verlag

About this paper

Cite this paper

Shahar, A., Reuveny, S. (1987). Nerve and muscle cells on microcarriers in culture. In: Vertrebrate Cell Culture I. Advances in Biochemical Engineering/Biotechnology, vol 34. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0000672

Download citation

  • DOI: https://doi.org/10.1007/BFb0000672

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-17626-8

  • Online ISBN: 978-3-540-47725-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation