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Recent Advances in Microbial Genome Sequencing

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Microbial Genomics in Sustainable Agroecosystems

Abstract

Microbes play significant roles in environmental ecological balance and human health. Microbes are associated with infection and immunity in humans. The microbial culturing and analysis has improved over the course of period with the advent of DNA sequencing technology. Since first bacterial genome of bacterium Haemophilus was sequenced by using Sanger method almost 20 years back, DNA sequencing technology has advanced drastically in capability and applications. With advances in next-generation sequencing technology involving improvement in the chemistry, increase in the output and quality of data, microbial genome sequencing has become an affordable approach to do comprehensive microbial analysis. The next-generation sequencing methods are more advanced and data throughput in a typical NGS method is about 100-fold higher compared to Sanger sequencing. The next-generation sequencing technology has revolutionized fields of genetics, genomics, microbiology, and clinical microbiology. Now whole genome microbial sequencing can be performed within few hours. With the third-generation sequencing like single-molecule sequencing, the possibility of having more of complete microbial genomes has increased. Analysis of complete genome sequences would provide a great insight about microbial diversity, virulence, evolution of microbes, and host-pathogen interactions. In this chapter, the recent advancements in the field of microbial genome sequencing have been discussed.

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Author information

Authors and Affiliations

  1. Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, Uttar Pradesh, India

    Rajpal Srivastav

  2. Department of Science and Technology, Ministry of Science and Technology, Government of India, New Delhi, India

    Rajpal Srivastav

  3. CSIR- Institute of Genomics and Integrative Biology, New Delhi, India

    Garima Suneja

  4. Academy of Scientific and Innovative Research, Ghaziabad, Uttar Pradesh, India

    Garima Suneja

Authors
  1. Rajpal Srivastav
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  2. Garima Suneja
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Corresponding author

Correspondence to Rajpal Srivastav .

Editor information

Editors and Affiliations

  1. Department of Molecular and Cellular Engineering, Sam Higginbottom University of Agriculture Technology and Sciences, Allahabad, Uttar Pradesh, India

    Vijay Tripathi

  2. Department of Forestry, North Eastern Regional Institute of Science and Technology (NERIST), Nirjuli, Arunachal Pradesh, India

    Pradeep Kumar

  3. Department of Computational Biology and Bioinformatics, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, Uttar Pradesh, India

    Pooja Tripathi

  4. Department of Botany, Kamla Nehru P.G. College, Raebareli, Uttar Pradesh, India

    Amit Kishore

  5. Department of Forestry, North Eastern Regional Institute of Science and Technology (NERIST), Nirjuli, Arunachal Pradesh, India

    Madhu Kamle

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Srivastav, R., Suneja, G. (2019). Recent Advances in Microbial Genome Sequencing. In: Tripathi, V., Kumar, P., Tripathi, P., Kishore, A., Kamle, M. (eds) Microbial Genomics in Sustainable Agroecosystems. Springer, Singapore. https://doi.org/10.1007/978-981-32-9860-6_8

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