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Microfluidic Technology for Single-Cell Manipulation

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Microfluidics for Single-Cell Analysis

Part of the book series: Integrated Analytical Systems ((ANASYS))

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Abstract

Single-cell analysis has attracted much attention in the field of biological and biomedical study owing to the heterogeneity among individual cells. This poses significant challenges to conventional bulk assays which would mask rare but important information owing to the assumption of average behavior. To avoid the interference of useless cells and obtain the single cells in the trial of genomics, proteomics, metabonomics, and single-cell behavior study, various cell manipulation techniques have been developed for single-cell research. In this chapter, we introduce the principles of droplet generation and single-cell encapsulation and review the latest achievements of cell manipulation technique by categorizing externally applied manipulation forces: microstructures, electrical, optical, magnetic, acoustic, and mechanical. This chapter will also introduce our latest work and provide important references and ideas for the development of droplet microfluidic-based single-cell manipulation.

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  1. Department of Chemistry, Tsinghua University, Beijing, 100084, People’s Republic of China

    Weifei Zhang, Nan Li & Jin-Ming Lin

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    Jin-Ming Lin

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Zhang, W., Li, N., Lin, JM. (2019). Microfluidic Technology for Single-Cell Manipulation. In: Lin, JM. (eds) Microfluidics for Single-Cell Analysis. Integrated Analytical Systems. Springer, Singapore. https://doi.org/10.1007/978-981-32-9729-6_4

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