Abstract
Clusterbean is an obligatory self-pollinating and inbreeding species and therefore, little variation in DNA polymorphism is expected among the cultivars. It has triploid endosperm and has more vegetative growth as compared to diploid counterparts. The endosperm tissue often shows a high degree of chromosomal variations, polyploidy, miotic irregularities, chromosome bridges and laggards. A brief detail of various techniques, viz. RAPD—random amplified polymorphic DNA; ISSR—inter simple sequence repeats; EST—expressed sequence tag markers; SCAR—sequence characterized amplified regions for amplification of specific band; CAPs—cleaved amplified polymorphic sequences; ITS—internal transcribed spacer; SSRs—Simple sequence repeats used for various purposes in clusterbean is summarized in this chapter.
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Pathak, R. (2015). Genetic Markers and Biotechnology. In: Clusterbean: Physiology, Genetics and Cultivation. Springer, Singapore. https://doi.org/10.1007/978-981-287-907-3_7
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