Skip to main content
SpringerLink
Log in
Book cover

Helicobacter pylori pp 371–378Cite as

Synopsis of Antimicrobial Resistance

  • Chapter
  • First Online:

  • 1931 Accesses

Abstract

Helicobacter pylori resistance to antibiotics is the main cause of failure of the eradication treatments. The essential mechanism of resistance acquisition is the occurrence of point mutations in genes important for the antimicrobial activity, while efflux pumps have also been described. Resistance to macrolides is steadily increasing around the world and is clinically significant. Besides, the standard methods, it can be detected in a few hours by molecular methods especially real-time PCR followed by a melting curve analysis. Resistance to fluoroquinolones is also important for the same reasons, and some molecular methods can also be applied. Metronidazole resistance is very common, but it can be overcome in vivo. Its in vitro detection lacks reproducibility, and no molecular method is currently available. Resistance to the other antibiotics of potential interest as for amoxicillin, tetracycline, and rifamycin is seldom found.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  1. Warren JR, Marshall B. Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet. 1983;1:1273–5.

    Google Scholar 

  2. Lind T, Veldhuyzen van Zanten S, Unge P, Spiller R, Bayerdorffer E, O’Morain C, et al. Eradication of Helicobacter pylori using one-week triple therapies combining omeprazole with two antimicrobials: the MACH I Study. Helicobacter. 1996;1:138–44.

    Article  CAS  PubMed  Google Scholar 

  3. Malfertheiner P, Megraud F, O’Morain CA, Atherton J, Axon AT, Bazzoli F, et al. Management of Helicobacter pylori infection – the Maastricht IV/Florence consensus report. Gut. 2012;61:646–64.

    Article  CAS  PubMed  Google Scholar 

  4. Rice LB. Genetics of resistance. In: Courvalin P, Leclercq R, Rice LB, editors. Antibiogram. Portland: ESKA Publishing/ASM Press; 2010. p. 25–36.

    Google Scholar 

  5. Dailidiene D, Bertoli MT, Miciuleviciene J, Mukhopadhyay AK, Ailide G, Pascasio MA, et al. Emergence of tetracycline resistance in Helicobacter pylori: multiple mutational changes in 16S ribosomal DNA and other genetic loci. Antimicrob Agents Chemother. 2002;46:3940–6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. van Amsterdam K, Bart A, van der Ende A. A Helicobacter pylori TolC efflux pump confers resistance to metronidazole. Antimicrob Agents Chemother. 2005;49:1477–82.

    Article  PubMed  PubMed Central  Google Scholar 

  7. Versalovic J, Shortridge D, Kibler K, Griffy MV, Beyer J, Flamm RK, et al. Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori. Antimicrob Agents Chemother. 1996;40:477–80.

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Occhialini A, Urdaci M, Doucet-Populaire F, Bébéar CM, Lamouliatte H, Mégraud F. Macrolide resistance in Helicobacter pylori: rapid detection of point mutations and assays of macrolide binding to ribosomes. Antimicrob Agents Chemother. 1997;41:2724–8.

    CAS  PubMed  PubMed Central  Google Scholar 

  9. Ménard A, Santos A, Mégraud F, Oleastro M. PCR-restriction fragment length polymorphism can also detect point mutation A2142C in the 23S rRNA gene, associated with Helicobacter pylori resistance to clarithromycin. Antimicrob Agents Chemother. 2002;46:1156–7.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Moore RA, Beckthold B, Wong S, Kureishi A, Bryan LE. Nucleotide sequence of the gyrA gene and characterization of ciprofloxacin-resistant mutants of Helicobacter pylori. Antimicrob Agents Chemother. 1995;39:107–11.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Tankovic J, Lascols C, Sculo Q, Petit JC, Soussy CJ. Single and double mutations in gyrA but not in gyrB are associated with low- and high-level fluoroquinolone resistance in Helicobacter pylori. Antimicrob Agents Chemother. 2003;47:3942–4.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Gerrits MM, Schuijffel D, van Zwet AA, Kuipers EJ, Vandenbroucke-Grauls CM, Kusters JG. Alterations in penicillin-binding protein 1A confer resistance to beta-lactam antibiotics in Helicobacter pylori. Antimicrob Agents Chemother. 2002;46:2229–33.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Dore MP, Graham DY, Sepulveda AR. Different penicillin-binding protein profiles in amoxicillin-resistant Helicobacter pylori. Helicobacter. 1999;4:154–61.

    Article  CAS  PubMed  Google Scholar 

  14. Gerrits MM, de Zoete MR, Arents NL, Kuipers EJ, Kusters JG. 16S rRNA mutation-mediated tetracycline resistance in Helicobacter pylori. Antimicrob Agents Chemother. 2002;46:2996–3000.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Trieber CA, Taylor DE. Mutations in the 16S rRNA genes of Helicobacter pylori mediate resistance to tetracycline. J Bacteriol. 2002;184:2131–40.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Heep M, Beck D, Bayerdörffer E, Lehn N. Rifampin and rifabutin resistance mechanism in Helicobacter pylori. Antimicrob Agents Chemother. 1999;43:1497–9.

    CAS  PubMed  PubMed Central  Google Scholar 

  17. Hoffman PS, Goodwin A, Johnsen J, Magee K, Veldhuyzen van Zanten SJ. Metabolic activities of metronidazole-sensitive and -resistant strains of Helicobacter pylori: repression of pyruvate oxidoreductase and expression of isocitrate lyase activity correlate with resistance. J Bacteriol. 1996;178:4822–9.

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Marais A, Bilardi C, Cantet F, Mendz GL, Mégraud F. Characterization of the genes rdxA and frxA involved in metronidazole resistance in Helicobacter pylori. Res Microbiol. 2003;154:137–44.

    Article  CAS  PubMed  Google Scholar 

  19. Glupczynski Y, Broutet N, Cantagrel A, Andersen LP, Alarcon T, López-Brea M, et al. Comparison of the E test and agar dilution method for antimicrobial susceptibility testing of Helicobacter pylori. Eur J Clin Microbiol Infect Dis. 2002;21:549–52.

    Article  CAS  PubMed  Google Scholar 

  20. Piccolomini R, Di Bonaventura G, Catamo G, Carbone F, Neri M. Comparative evaluation of the E test, agar dilution, and broth microdilution for testing susceptibilities of Helicobacter pylori strains to 20 antimicrobial agents. J Clin Microbiol. 1997;35:1842–6.

    CAS  PubMed  PubMed Central  Google Scholar 

  21. Coudron PE, Stratton CW. Factors affecting growth and susceptibility testing of Helicobacter pylori in liquid media. J Clin Microbiol. 1995;33:1028–30.

    CAS  PubMed  PubMed Central  Google Scholar 

  22. Mégraud F, Bénéjat L, Ontsira Ngoyi EN, Lehours P. Molecular approaches to identify Helicobacter pylori antimicrobial resistance. Gastroenterol Clin N Am. 2015;44:577–96.

    Article  Google Scholar 

  23. Oleastro M, Ménard A, Santos A, Lamouliatte H, Monteiro L, Barthélémy P, et al. Real-time PCR assay for rapid and accurate detection of point mutations conferring resistance to clarithromycin in Helicobacter pylori. J Clin Microbiol. 2003;41:397–402.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Schabereiter-Gurtner C, Hirschl AM, Dragosics B, Hufnagl P, Puz S, Kovách Z, et al. Novel real-time PCR assay for detection of Helicobacter pylori infection and simultaneous clarithromycin susceptibility testing of stool and biopsy specimens. J Clin Microbiol. 2004;42:4512–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Monteiro L, Gras N, Vidal R, Cabrita J, Megraud F. Detection of Helicobacter pylori DNA in human feces by PCR: DNA stability and removal of inhibitors. J Microbiol Methods. 2001;45:89–94.

    Article  CAS  PubMed  Google Scholar 

  26. Glocker E, Kist M. Rapid detection of point mutations in the gyrA gene of Helicobacter pylori conferring resistance to ciprofloxacin by a fluorescence resonance energy transfer-based real-time PCR approach. J Clin Microbiol. 2004;42:2241–6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Megraud F. H. pylori antibiotic resistance: prevalence, importance, and advances in testing. Gut. 2004;53:1374–84.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Megraud F, Coenen S, Versporten A, Kist M, Lopez-Brea M, Hirschl AM, et al. Helicobacter pylori resistance to antibiotics in Europe and its relationship to antibiotic consumption. Gut. 2013;62:34–42.

    Article  CAS  PubMed  Google Scholar 

  29. Camargo MC, Garcia A, Riquelme A, Otero W, Camargo CA, Hernandez-Garcia T, et al. The problem of Helicobacter pylori resistance to antibiotics: a systematic review in Latin America. Am J Gastroenterol. 2014;109:485–95.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Lee JW, Kim N, Kim JM, Nam RH, Chang H, Kim JY, et al. Prevalence of primary and secondary antimicrobial resistance of Helicobacter pylori in Korea from 2003 through 2012. Helicobacter. 2013;18:206–14.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Inserm U853, Université de Bordeaux, Laboratoire de Bactériologie, Bordeaux, France

    Francis Mégraud MD

Authors
  1. Francis Mégraud MD
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Francis Mégraud MD .

Editor information

Editors and Affiliations

  1. Seoul National University, Bundang Hospital, Seongnam-si, Gyeonggi-do, Korea (Republic of)

    Nayoung Kim

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer Science+Business Media Singapore

About this chapter

Cite this chapter

Mégraud, F. (2016). Synopsis of Antimicrobial Resistance. In: Kim, N. (eds) Helicobacter pylori. Springer, Singapore. https://doi.org/10.1007/978-981-287-706-2_35

Download citation

  • DOI: https://doi.org/10.1007/978-981-287-706-2_35

  • Published:

  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-287-705-5

  • Online ISBN: 978-981-287-706-2

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics

Search

Navigation