Abstract
The transformation efficiency of japonica rice cv. Taichung 65 using Agrobacterium tumefaciens was investigated. Mature rice seeds were cultured on N6D medium for callus induction. Scutellum-derived calli were transformed with Agrobacterium strain AGL1, harboring binary vector pCAMBIA 1305.1 which contains gusA as a reporter gene and hptII as a selectable marker gene. After co-cultivation, it was found that calli showed transient expression of gusA gene. The transformed calli were selected on medium containing hygromycin and cefotaxime for two cycles of 2 weeks each. Then hygromycin-resistant calli were regenerated to plantlets. PCR analysis confirmed the presence of gusA and hptII genes in the genome of transgenic rice plants. Transformation efficiency of Taichung 65 in this study was 4.32 %. The results will be useful to establish transformation system for studies of gene function and genetic improvement of rice varieties.
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This research was supported by the grant from National Research Council of Thailand.
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Sakulsingharoj, C., Phanlumpak, K., Inta, P., Pongjaroenkit, S., Sangtong, V. (2015). Transformation of Rice (Oryza sativa) Cultivar Taichung 65 Mediated by Agrobacterium tumefaciens . In: Gnanamalar Sarojini Daniel, E. (eds) Biology Education and Research in a Changing Planet. Springer, Singapore. https://doi.org/10.1007/978-981-287-524-2_20
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DOI: https://doi.org/10.1007/978-981-287-524-2_20
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