Abstract
The cellulase-producing microorganisms were isolated from the soil, compost, cow dung, decaying wood, and microbial activator. One hundred and sixty isolates of bacteria, seven isolates of actinomyces, and 12 isolates of fungi were obtained. Additional 73 bacterial isolates used in this study were a collection from microbiological laboratory of Maejo University, and one was obtained from Thailand Institute of Scientific and Technological Research (TISTR). Sixteen fungi from TISTR were included. The primary screening of cellulase production of bacteria was done on agar medium. Thirty-five selected bacteria, seven actinomyces, and 12 fungi were determined for cellulase activity in CMC broth. Among these microorganisms, Aspergillus foetidus TISTR 3159 is the most efficient cellulase producer, and its enzyme activity in the crude sample was found to be 0.053 U/ml (specific activity 0.323 U/mg protein). The growth and cellulase production of A. foetidus TISTR 3159 in CMC broth showed the highest cellulase activity 0.051 U/ml (specific activity 0.340 U/mg protein) for 84 h. The substrate specificity of crude enzyme from A. foetidus TISTR 3159 on various substrates was also examined. It showed the highest enzyme activity (0.053 U/ml) and specific activity on CMC (0.324 U/mg protein).
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Deejing, S., Dittamart, D. (2015). Isolation and Screening of Cellulase-Producing Microorganisms and the Study of Some Characteristics of Enzymes. In: Gnanamalar Sarojini Daniel, E. (eds) Biology Education and Research in a Changing Planet. Springer, Singapore. https://doi.org/10.1007/978-981-287-524-2_16
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DOI: https://doi.org/10.1007/978-981-287-524-2_16
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