Abstract
The absolute necessity for rational therapy in the face of rampant drug resistance places increasing importance on the accuracy of malaria diagnosis. Giemsa microscopy and Triple test method Rapid Diagnostic Test (RDT) represent the two diagnoses most likely to have the largest impact on malaria control in this present study. These two methods have their characteristic strengths and limitation. These tests were carried out on 156 patients of endemic areas of Gajapati district of Odisha State. These tests were evaluated by two methods, i.e. microscopic slide test and RDT kit method. RDT kits belong to Advantage MAL CARD Malaria pLDH (Plasmodium Lactate Dehydrogenase) antibody Pre-coated (J. Mitra & Co, India) card test, ParaHIT PfHRP2 (Histidine-Rich Protein 2) antibody pre-coated dip stick (Span Diagnostic Ltd, India) and S.D BIOLINE pf/pv capture antigen MSP (Merozoite Surface Protein) pre-coated card (S.D. BIO Standard Diagnostic Pvt. Ltd, India). A little amount of whole blood (5 μl) was taken using the plastic loop given with the kit, at the same time two slides are also taken. Out of 156 patients it was detected that 128 were negative and 28 were positive according to microscopic detection. Out of these 28 positives 23 were only P. falciparum, 2 were only P. vivax, 3 were having both PF and PV infection detected. On the other hand 32 were positive and 4 were false positive as shown by Advantage MAL CARD and ParaHIT, where as 49 were positive and 21 were false positive by the S.D. MSP pre-coated antigen card test kits.
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Acknowledgments
We acknowledge the Chief District Medical Officer, Department of Health, District Gajapati and kind cooperation of Community Health Centres medical officers, health workers and officials, for their help in Blood sample collection, Patient data collection, processing and analysis.
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Panda, S.K., Kumar, A. (2015). Rapid Diagnostic Tests Show False Positive Leading to Dilemma in Malarial Treatment: A Case Study. In: Biotechnology and Bioforensics. SpringerBriefs in Applied Sciences and Technology(). Springer, Singapore. https://doi.org/10.1007/978-981-287-050-6_8
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