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Functional Analysis of Plant Genes Related to Arbuscular Mycorrhiza Symbiosis Using Agrobacterium rhizogenes-Mediated Root Transformation and Hairy Root Production

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Hairy Root Cultures Based Applications

Part of the book series: Rhizosphere Biology ((RHBIO))

Abstract

Arbuscular mycorrhizal symbiosis is a mutualistic endosymbiosis widely distributed in the plant kingdom which has a significant impact on plant growth and health. Agrobacterium rhizogenes-mediated root transformation and composite plant generation have been described as a rapid method to assess gene functions in roots without the need for stable transformation plant production. We describe an optimized protocol for composite tomato plant obtaining achieved through A. rhizogenes-mediated transformation, and we also highlight key differences with other protocols that should be taken into account to adjust this method to the transformation of other plant species. This protocol has been adopted as a useful tool for localizing the promoter expression of genes putatively associated with mycorrhization or for functional analyses in mycorrhizal studies by reverse genetics.

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Acknowledgements

This work was supported by grants from the Comisión Interministerial de Ciencia y Tecnología (CICYT) and Fondos Europeos de Desarrollo Regional (FEDER) through the Ministerio de Economía, industria y Competitividad in Spain (AGL2014-52298-P, AGL2017-83871-P).

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Authors and Affiliations

  1. Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (EEZ), CSIC, Granada, Spain

    Tania Ho-Plágaro, María Isabel Tamayo-Navarrete & José Manuel García-Garrido

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  1. Tania Ho-Plágaro
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  2. María Isabel Tamayo-Navarrete
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  3. José Manuel García-Garrido
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Correspondence to José Manuel García-Garrido .

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Editors and Affiliations

  1. Department of Botany, Central University of Jammu, Samba, Jammu and Kashmir, India

    Vikas Srivastava

  2. Department of Biotechnology, Institute of Engineering and Technology, Lucknow, Uttar Pradesh, India

    Shakti Mehrotra

  3. School of Biotechnology, University of Jammu, Jammu, Jammu and Kashmir, India

    Sonal Mishra

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Ho-Plágaro, T., Tamayo-Navarrete, M.I., García-Garrido, J.M. (2020). Functional Analysis of Plant Genes Related to Arbuscular Mycorrhiza Symbiosis Using Agrobacterium rhizogenes-Mediated Root Transformation and Hairy Root Production. In: Srivastava, V., Mehrotra, S., Mishra, S. (eds) Hairy Root Cultures Based Applications. Rhizosphere Biology. Springer, Singapore. https://doi.org/10.1007/978-981-15-4055-4_13

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