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Cryopreservation and Storage of Oocytes, Embryos and Embryonic Cells of Fish

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Cryopreservation of Fish Gametes

Abstract

Worldwide hatchery seed production has seen a manifold growth in terms of quantity of seed produced due to advancements in reproductive biotechnology and brood stock management. However, the quality of seed is an important parameter for profitable aquaculture. Both male and female gametes and stored embryos of animals play prominent roles in the genetic management of species and conservation. However, storage by cryopreservation of oocytes or embryos of fish like carps and other teleost is elusive and few claims are not reproducible in nature. Production of improved stock using cryopreserved milt is being practiced in many hatcheries. Male gametes of improved stock are cryopreserved and utilized for quality seed production as well as upgrading the brood stock in carps. However, cryogenic and non-cryogenic storage of oocytes and embryos has not been possible due to several reasons. Researchers have successfully cryopreserved and revived embryonic cells of fish in many laboratories around the world. Here an attempt has been made to describe the principles, practice, issues and way forward in the field of cryopreservation of fish egg (oocytes), embryo and embryonic cells both for long-term and short-term duration storage.

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Authors and Affiliations

  1. Cryobiology Laboratory, ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, India

    P. Routray

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  1. Department of Aquaculture, Fisheries College and Research Institute, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Tuticorin, Tamil Nadu, India

    Judith Betsy

  2. Directorate of Sustainable Aquaculture, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Thanjavur, Tamil Nadu, India

    Stephen Kumar

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Routray, P. (2020). Cryopreservation and Storage of Oocytes, Embryos and Embryonic Cells of Fish. In: Betsy, J., Kumar, S. (eds) Cryopreservation of Fish Gametes. Springer, Singapore. https://doi.org/10.1007/978-981-15-4025-7_13

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