Abstract
This study was devoted to the learning changes in the structure of erythrocyte membranes at the level of molecular bonds during their storage at a positive temperature by means method of infrared spectroscopy (IR). Objects of research were red blood cells (RBCs) into bags containing preservative CPD and RBCs into bags containing preservative CPDA-1. As membrane protective used saline which had previously been treated with magnetite nanoparticles (ICNB) by the Belousov’s method. The physiological solution that was treated with nanoparticles was added to the preserved RBCs according to the developed method. Sample of control was the addition of intact saline. Analysis of changes occurring in the IR spectra of samples of control and test in the CPD medium was showed that during the first 28 days storage of: (1) In the suspension of control of the RBCs, noticeable destructive changes in the molecular structure membrane of erythrocytes at the 14th day storage begins. After three weeks, the molecular structure membrane of erythrocytes is completely destroyed; (2) In the sample of test there was a weakening and rupture of molecular bonds only at the 28th day storage of RBCs. Complete destruction of the structure of membrane of erythrocytes occurs at the 35th day storage. Analysis of changes occurring in the IR spectra of samples of control and test in the CPDA-1 medium was showed that during 49 days storage of: (1) In the suspension of control of RBCs noticeable destructive changes in the molecular structure begins in four weeks, and after six weeks storage the molecular structure of erythrocytes membrane are completely destroyed; (2) In the sample of test, a significant weakening of intra-and intermolecular bonds in the structure of erythrocytes membrane occurs after six weeks. However, the complete destruction of the structure is not observed. After seven weeks storage of erythrocytes obvious violations of the molecular structure of lipids and proteins that make up the RBCs are visible but some of the strongest compounds still remain. In general, the results clearly showed that the presented method of application of nanotechnology significantly increases the storage time of RBCs in different versions of preservatives due to mechanisms to reduce violations of the molecular structure of proteins and lipids in the erythrocyte membranes. Presented method of application of nanotechnology is not only safe for use in practice in the Blood Service, Transfusiology and Hematology, but also is the most promising innovation project.
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References
J.R. Hess, Vox Sang. 91, 13–19 (2006)
C.F. Högman, H.T. Meryman, Transfus. Med. Rev. 13, 275–296 (1999)
J.R. Hess, Transfus Apher Sci. 43, 51–59 (2010)
A.B. Zimrin, J.R. Hess, Vox Sang. 96, 93–103 (2009)
L. Van de Watering, Vox Sang. 100, 36–45 (2011)
D. Wang, J. Sun, S.B. Solomon et al., Transfusion. (2012). https://doi.org/10.1111/j.1537-2995.2011.03466.x
S.A. Glynn, Transfusion 50, 1164–1169 (2010)
G.M. D’Amici, S. Rinalducci, L. Zolla, J. Proteome Res. 6, 3242–3255 (2007)
A. Verhoeven, P. Hilarius, D. Dekkers et al., Vox Sang. 91, 244–251 (2006)
R.L. Sparrow, M.F. Veale, G. Healey, K.A. Payne, Transfusion 47, 966–968 (2007)
G.J. Bosman, E. Lasonder, Y.A. Groenen-Döpp et al., J. Proteomics 73, 396–402 (2010)
A.N. Belousov, The use of magnetite nanoparticles in applied medicine. Int. J. Nano Dimens. 2(1(5)), 25–28. Print Edition ISSN: 2008-8868. Web Edition ISSN: 2228-5059
A.N. Belousov, E.Yu. Belousova, The first steps in discovery new mechanisms of cellular regulation in means by nanotechnology preparations, in X International Conference New Information Technologies in Medicine and Ecology, Yalta, Gursuf (2002), pp. 420–425
A.N. Belousov, E.Yu, Belousova Mechanisms cell regulation by nanotechnology preparations (MCS-B), in BioNanotech Conference & Expo 2012, Santa Clura, CA, USA, 18–21 June 2012
A.N. Belousov, Effect of Magnet controlled sorbent on parameters of acid-base balance of the blood and the processes of glycolysis in erythrocytes. Pain Anesthesia Intens Care 1, 263–265 (2000)
A.N. Belousov, V.P. Nevzorov Ultrastructure of cells in the kidneys and lungs of rabbits after administration of magnetite, in International Collection of Scientific Papers IV Scientific and Practical Conference on the Creation and Testing of New Drugs, Moscow, vol. 4 (1997), pp. 77–87
A.N. Belousov, V.P. Nevzorov Ultrastructure of liver cells after administration of magnetite, in International Collection of Scientific Papers IV Scientific and Practical Conference on the Creation and Testing of New Drugs, Moscow, vol. 4 (1997), pp. 71–77
A.N. Belousov, Spectrum of application magnetite nanopaticles in medicine, in Nanotech, vol. 2 (2009), pp. 154–157. ISBN: 978-1-4398-1783-4
A.N. Belousov, Effect on hemolysis and transport ATPase activity of erythrocytes by means nanopareticles of magnetit controlled sorbent (MCS-B). Pain Anesthesia Intens. Care 1, 26–28
A.N. Belousov, J. Cell, Mol. Biol. 2(1), 1–4 (2017)
A.N. Belousov, Application magnetite of nanoparticles (ICNB Preparation) as magnetically-resonant contrasting means during visualization of tumours. Clean Technol. Sustain. Ind. Organ. 10, 379–381. ISBN: 978-1-4822-5819-6
A.N. Belousov, Prospect. Med. Biol. 1, 94–97 (2012)
A.N. Belousov, Biomed. Eng. Res. 2(3), 147–152 (2014)
A.N. Belousov, Nanomater. Mol. Nanotechnol. 14–25, 17–21 (2014)
A.N. Belousov, J. Mater. Sci. Eng. A 4(11), 367–372 (2014)
A.N. Belousov, E. Malygon, V.V. Yavorskiy, E. Belousova, New effective method preservation of for red blood cells by means of nanotechnology. Nanomed. Nanotechnol. 3(3), 000149 (2018). ISSN: 2574-187X. https://doi.org/10.23880/nnoa-16000149
A.N. Belousov, E. Malygon, V.V. Yavorskiy, E. Belousova, Int. J. Nanotechnol. Nanomed. 3(2), 1–8 (2018)
A.N. Belousov, E. Malygon, V.V. Yavorskiy, E. Belousova, Int. J. Hematol. Blood Disorders. Symbiosis Group 3(2), 1–9 (2018)
L.A. Kozitsina, N.B. Kupletskaya, Application of UF, IR and IMR Spectroscopy in Organic Chemistry (1971), 213p
A. Cross, Introduction to Practical Infrared Spectroscopy (Publishing House for Foreign Literature, Moscow, 1961), 110p
A.A Babushkin, P.A. Bazhulin, F.A. Korolev et.al., Methods of Spectral Analysis (Publishing House of the Moscow University, Moscow, 1962), 509p
L.A. Gribov, Introduction to the Theory and Calculation of Vibrational Spectra of Many Molecules (Ed. LGI, 1965), 134p
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Belousov, A.N., Malygon, E.I., Yavorskiy, V.V., Belousova, E.Y. (2020). Application of Infrared Spectroscopy to Study the Effect of Magnetite Nanoparticles (ICNB) on Molecular Structure of the Membranes of Preserved RBCs. In: Pogrebnjak, A., Pogorielov, M., Viter, R. (eds) Nanomaterials in Biomedical Application and Biosensors (NAP-2019). Springer Proceedings in Physics, vol 244. Springer, Singapore. https://doi.org/10.1007/978-981-15-3996-1_4
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