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Decellularized Extracellular Matrices for Tissue Engineering and Regeneration

  • Fang Ge
  • Yuhe Lu
  • Qian Li
  • Xing ZhangEmail author
Chapter
  • 98 Downloads
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1250)

Abstract

Decellularized extracellular matrices (dECMs) from mammalian tissues and organs are particularly interesting as scaffolds for tissue engineering and regeneration when considering their ability to retain chemical compositions and three-dimensional (3D) microstructures that are similar to native ECMs. This review discusses the advantages and disadvantages of different decellularization methods that use various agents, such as ionic and nonionic detergents and biological enzymes. The applications of dECMs as scaffolds or hydrogels for tissue engineering of specific tissues including heart valves, blood vessels, and skin, as well as their performance in vitro and in vivo, are also discussed. In addition, whole organ regeneration (i.e., the heart, kidney, liver) using dECM scaffolds has been explored, which are able to recapitulate partial functions of native organs.

Keywords

Decellularization Recellularization Extracellular matrices Microstructure Tissue engineering Organ regeneration 

References

  1. 1.
    Crapo PM, Gilbert TW, Badylak SF (2011) An overview of tissue and whole organ decellularization processes. Biomaterials 32(12):3233–3243PubMedPubMedCentralGoogle Scholar
  2. 2.
    Gálvez-Montón C, Prat-Vidal C, Roura S et al (2013) Cardiac tissue engineering and the bioartificial heart. Rev Esp Cardiol 66(5):391–399PubMedGoogle Scholar
  3. 3.
    Kappetein AP, Feldman TE, Mack MJ et al (2011) Comparison of coronary bypass surgery with drug-eluting stenting for the treatment of left main and/or three-vessel disease: 3-year follow-up of the SYNTAX trial. Eur Heart J 32(17):2125–2134PubMedGoogle Scholar
  4. 4.
    L’Heureux N, Dusserre N, Konig G et al (2006) Human tissue-engineered blood vessels for adult arterial revascularization. Nat Med 12(3):361–365PubMedPubMedCentralGoogle Scholar
  5. 5.
    Mangold S, Schrammel S, Huber G et al (2015) Evaluation of decellularized human umbilical vein (HUV) for vascular tissue engineering – comparison with endothelium-denuded HUV. J Tissue Eng Regen Med 9(1):13–23PubMedGoogle Scholar
  6. 6.
    Moroni F, Mirabella T (2014) Decellularized matrices for cardiovascular tissue engineering. Am J Stem Cells 3(1):1–20PubMedPubMedCentralGoogle Scholar
  7. 7.
    Vorotnikova E, McIntosh D, Dewilde A et al (2010) Extracellular matrix-derived products modulate endothelial and progenitor cell migration and proliferation in vitro and stimulate regenerative healing in vivo. Matrix Biol 29(8):690–700PubMedGoogle Scholar
  8. 8.
    Lutolf MR, Weber FE, Schmoekel HG et al (2003) Repair of bone defects using synthetic mimetics of collagenous extracellular matrices. Nat Biotechnol 21(5):513–518PubMedGoogle Scholar
  9. 9.
    Bloch O, Golde P, Dohmen PM et al (2011) Immune response in patients receiving a bioprosthetic heart valve: lack of response with decellularized valves. Tissue Eng Part A 17(19–20):2399–2405PubMedGoogle Scholar
  10. 10.
    Xu CC, Chan RW, Tirunagari N (2007) A biodegradable, acellular xenogeneic scaffold for regeneration of the vocal fold lamina propria. Tissue Eng 13(3):551–566PubMedGoogle Scholar
  11. 11.
    Mann BK, Gobin AS, Tsai AT et al (2001) Smooth muscle cell growth in photopolymerized hydrogels with cell adhesive and proteolytically degradable domains: synthetic ECM analogs for tissue engineering. Biomaterials 22(22):3045–3051PubMedGoogle Scholar
  12. 12.
    Badylak SF (2002) The extracellular matrix as a scaffold for tissue reconstruction. Semin Cell Dev Biol 13(5):377–383PubMedGoogle Scholar
  13. 13.
    Badylak SF (2004) Xenogeneic extracellular matrix as a scaffold for tissue reconstruction. Transpl Immunol 12(3–4):367–377PubMedGoogle Scholar
  14. 14.
    Jarvelainen H, Sainio A, Koulu M et al (2009) Extracellular matrix molecules: potential targets in pharmacotherapy. Pharmacol Rev 61(2):198–223PubMedPubMedCentralGoogle Scholar
  15. 15.
    Liang R, Fisher M, Yang G et al (2011) Alpha1,3-galactosyltransferase knockout does not alter the properties of porcine extracellular matrix bioscaffolds. Acta Biomater 7(4):1719–1727PubMedGoogle Scholar
  16. 16.
    Hong Y, Huber A, Takanari K et al (2011) Mechanical properties and in vivo behavior of a biodegradable synthetic polymer microfiber-extracellular matrix hydrogel biohybrid scaffold. Biomaterials 32(13):3387–3394PubMedPubMedCentralGoogle Scholar
  17. 17.
    Lee KY, Bouhadir KH, Mooney DJ (2004) Controlled degradation of hydrogels using multi-functional cross-linking molecules. Biomaterials 25(13):2461–2466PubMedGoogle Scholar
  18. 18.
    Kloxin AM, Tibbitt MW, Anseth KS (2010) Synthesis of photodegradable hydrogels as dynamically tunable cell culture platforms. Nat Protoc 5(12):1867–1887PubMedPubMedCentralGoogle Scholar
  19. 19.
    Bejleri D, Davis ME (2019) Decellularized extracellular matrix materials for cardiac repair and regeneration. Adv Healthc Mater 8(5):1801217Google Scholar
  20. 20.
    Wolf MT, Daly KA, Brennan-Pierce EP et al (2012) A hydrogel derived from decellularized dermal extracellular matrix. Biomaterials 33(29):7028–7038PubMedPubMedCentralGoogle Scholar
  21. 21.
    Badylak SF, Freytes DO, Gilbert TW (2009) Extracellular matrix as a biological scaffold material: structure and function. Acta Biomater 5(1):1–13PubMedGoogle Scholar
  22. 22.
    Gilbert TW, Sellaro TL, Badylak SF (2006) Decellularization of tissues and organs. Biomaterials 27(19):3675–3683PubMedGoogle Scholar
  23. 23.
    Phillips M, Maor E, Rubinsky B (2010) Nonthermal irreversible electroporation for tissue decellularization. J Biomech Eng 132(9):091003PubMedGoogle Scholar
  24. 24.
    Hashimoto Y, Funamoto S, Sasaki S et al (2010) Preparation and characterization of decellularized cornea using high-hydrostatic pressurization for corneal tissue engineering. Biomaterials 31(14):3941–3948PubMedGoogle Scholar
  25. 25.
    Zhou J, Fritze O, Schleicher M et al (2010) Impact of heart valve decellularization on 3-D ultrastructure, immunogenicity and thrombogenicity. Biomaterials 31(9):2549–2554PubMedGoogle Scholar
  26. 26.
    Assmann A, Delfs C, Munakata H et al (2013) Acceleration of autologous in vivo recellularization of decellularized aortic conduits by fibronectin surface coating. Biomaterials 34(25):6015–6026PubMedGoogle Scholar
  27. 27.
    Dong J, Li Y, Mo X (2013) The study of a new detergent (octyl-glucopyranoside) for decellularizing porcine pericardium as tissue engineering scaffold. J Surg Res 183(1):56–67PubMedGoogle Scholar
  28. 28.
    Seebacher G, Grasl C, Stoiber M et al (2008) Biomechanical properties of decellularized porcine pulmonary valve conduits. Artif Organs 32(1):28–35PubMedGoogle Scholar
  29. 29.
    Seo Y, Jung Y, Kim SH (2018) Decellularized heart ECM hydrogel using supercritical carbon dioxide for improved angiogenesis. Acta Biomater 67:270–281PubMedGoogle Scholar
  30. 30.
    Akhyari P, Aubin H, Gwanmesia P et al (2011) The quest for an optimized protocol for whole-heart decellularization: a comparison of three popular and a novel decellularization technique and their diverse effects on crucial extracellular matrix qualities. Tissue Eng Part C Methods 17(9):915–926PubMedGoogle Scholar
  31. 31.
    Sarig U, Au-Yeung GC, Wang Y et al (2012) Thick acellular heart extracellular matrix with inherent vasculature: a potential platform for myocardial tissue regeneration. Tissue Eng Part A 18(19–20):2125–2137PubMedPubMedCentralGoogle Scholar
  32. 32.
    Conconi MT, Coppi PD, Liddo RD et al (2005) Tracheal matrices, obtained by a detergent-enzymatic method, support in vitro the adhesion of chondrocytes and tracheal epithelial cells. Transpl Int 18(6):727–734PubMedGoogle Scholar
  33. 33.
    Conconi MT, Coppi PD, Bellini S et al (2005) Homologous muscle acellular matrix seeded with autologous myoblasts as a tissue-engineering approach to abdominal wall-defect repair. Biomaterials 26(15):2567–2574PubMedGoogle Scholar
  34. 34.
    Theodoridis K, Tudorache I, Calistru A et al (2015) Successful matrix guided tissue regeneration of decellularized pulmonary heart valve allografts in elderly sheep. Biomaterials 52:221–228PubMedGoogle Scholar
  35. 35.
    Schenke-Layland K, Vasilevski O, Opitz F et al (2003) Impact of decellularization of xenogeneic tissue on extracellular matrix integrity for tissue engineering of heart valves. J Struct Biol 143(3):201–208PubMedGoogle Scholar
  36. 36.
    Lim HG, Kim SH, Choi SY et al (2012) Anticalcification effects of decellularization, solvent, and detoxification treatment for genipin and glutaraldehyde fixation of bovine pericardium. Eur J Cardiothorac Surg 41(2):383–390PubMedGoogle Scholar
  37. 37.
    Simona P, Kasimira MT, Seebachera G et al (2003) Early failure of the tissue engineered porcine heart valve SYNERGRAFT (TM) in pediatric patients. Eur J Cardiothorac Surg 23(6):1002–1006Google Scholar
  38. 38.
    Li Z, Guan J (2011) Hydrogels for cardiac tissue engineering. Polymers 3(4):740–761Google Scholar
  39. 39.
    Singelyn JM, DeQuach JA, Seif-Naraghi SB et al (2009) Naturally derived myocardial matrix as an injectable scaffold for cardiac tissue engineering. Biomaterials 30(29):5409–5416PubMedPubMedCentralGoogle Scholar
  40. 40.
    Uriel S, Labay E, Francis-Sedlak M et al (2009) Extraction and assembly of tissue-derived gels for cell culture and tissue engineering. Tissue Eng Part C Methods 15(3):309–321PubMedGoogle Scholar
  41. 41.
    Kleinman HK, Martin GR (2005) Matrigel: basement membrane matrix with biological activity. Semin Cancer Biol 15(5):378–386PubMedGoogle Scholar
  42. 42.
    Morris AH, Stamer DK, Kunkemoeller B et al (2018) Decellularized materials derived from TSP2-KO mice promote enhanced neovascularization and integration in diabetic wounds. Biomaterials 169:61–71PubMedPubMedCentralGoogle Scholar
  43. 43.
    Morris AH, Lee H, Xing H et al (2018) Tunable hydrogels derived from genetically engineered extracellular matrix accelerate diabetic wound healing. ACS Appl Mater Interfaces 10(49):41892–41901PubMedGoogle Scholar
  44. 44.
    Schaner PJ, Martin ND, Tulenko TN et al (2004) Decellularized vein as a potential scaffold for vascular tissue engineering. J Vasc Surg 40(1):146–153PubMedGoogle Scholar
  45. 45.
    Song JJ, Ott HC (2011) Organ engineering based on decellularized matrix scaffolds. Trends Mol Med 17(8):424–432PubMedGoogle Scholar
  46. 46.
    Taylor PM, Cass AEG, Yacoub MH (2006) Extracellular matrix scaffolds for tissue engineering heart valves. Prog Pediatr Cardiol 21(2):219–225Google Scholar
  47. 47.
    Simsa R, Padma AM, Heher P et al (2018) Systematic in vitro comparison of decellularization protocols for blood vessels. PLoS One 13(12):e0209269PubMedPubMedCentralGoogle Scholar
  48. 48.
    Lee PH, Tsai SH, Kuo L et al (2012) A prototype tissue engineered blood vessel using amniotic membrane as scaffold. Acta Biomater 8(9):3342–3348PubMedGoogle Scholar
  49. 49.
    Woods T, Gratzer PF (2005) Effectiveness of three extraction techniques in the development of a decellularized bone-anterior cruciate ligament-bone graft. Biomaterials 26(35):7339–7349PubMedGoogle Scholar
  50. 50.
    Roderjan JG, de Noronha L, Stimamiglio MA et al (2019) Structural assessments in decellularized extracellular matrix of porcine semilunar heart valves: evaluation of cell niches. Xenotransplantation 26(3):e12503PubMedGoogle Scholar
  51. 51.
    Wilczek P (2010) Heart valve bioprothesis: effect of different acellularizations methods on the biomechanical and morphological properties of porcine aortic and pulmonary valve. B Pol Acad Sci-Tech 58(2):337–342Google Scholar
  52. 52.
    Weymann A, Schmack B, Okada T et al (2013) Reendothelialization of human heart valve neoscaffolds using umbilical cord-derived endothelial cells. Circ J 77(1):207–216PubMedGoogle Scholar
  53. 53.
    Collatusso C, Roderjan JG, Vieira ED et al (2011) Decellularization as an anticalcification method in stentless bovine pericardium valve prosthesis: a study in sheep. Rev Bras Cir Cardiovasc 26(3):419–426PubMedGoogle Scholar
  54. 54.
    Rice RD, Ayubi FS, Shaub ZJ et al (2010) Comparison of Surgisis, AlloDerm, and Vicryl Woven Mesh grafts for abdominal wall defect repair in an animal model. Aesthet Plast Surg 34(3):290–296Google Scholar
  55. 55.
    Ott HC, Matthiesen TS, Goh SK et al (2008) Perfusion-decellularized matrix: using nature’s platform to engineer a bioartificial heart. Nat Med 14(2):213–221PubMedGoogle Scholar
  56. 56.
    Ng SL, Narayanan K, Gao S et al (2011) Lineage restricted progenitors for the repopulation of decellularized heart. Biomaterials 32(30):7571–7580PubMedGoogle Scholar
  57. 57.
    Taylor DA, Frazier OH, Elgalad A et al (2018) Building a total bioartificial heart: harnessing nature to overcome the current hurdles. Artif Organs 42(10):970–982PubMedGoogle Scholar
  58. 58.
    Orlando G, Booth C, Wang Z et al (2013) Discarded human kidneys as a source of ECM scaffold for kidney regeneration technologies. Biomaterials 34(24):5915–5925PubMedGoogle Scholar
  59. 59.
    Ross EA, Williams MJ, Hamazaki T et al (2009) Embryonic stem cells proliferate and differentiate when seeded into kidney scaffolds. J Am Soc Nephrol 20(11):2338–2347PubMedPubMedCentralGoogle Scholar
  60. 60.
    Abolbashari M, Agcaoili SM, Lee MK et al (2016) Repopulation of porcine kidney scaffold using porcine primary renal cells. Acta Biomater 29:52–61PubMedGoogle Scholar
  61. 61.
    Zhou P, Lessa N, Estrada DC et al (2011) Decellularized liver matrix as a carrier for the transplantation of human fetal and primary hepatocytes in mice. Liver Transpl 17(4):418–427PubMedPubMedCentralGoogle Scholar
  62. 62.
    Bao J, Wu Q, Sun J et al (2015) Hemocompatibility improvement of perfusion-decellularized clinical-scale liver scaffold through heparin immobilization. Sci Rep 5:10756PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2020

Authors and Affiliations

  1. 1.Institute of Metal ResearchChinese Academy of SciencesShenyangChina
  2. 2.Department of ChemistryNortheastern UniversityShenyangChina
  3. 3.School of Materials Science and EngineeringNortheastern UniversityShenyangChina
  4. 4.School of Materials Science and EngineeringUniversity of Science and Technology of ChinaHefeiChina

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