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Regulation of Stem Cell Functions by Micro-Patterned Structures

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1250))

Abstract

Micro-patterned surfaces have been broadly used to control the morphology of stem cells for investigation of the influence of physiochemical and biological cues on stem cell functions. Different structures of micro-patterned surfaces can be prepared by photolithography through designing the photomask features. Cell spreading area, geometry, aspect ratio, and alignment can be regulated by the micro-patterned structures. Their influences on adipogenic, osteogenic, and smooth muscle differentiation of the human bone marrow-derived mesenchymal stem cells are compared and investigated in details. Variation of cell morphology can trigger rearrangement of cytoskeleton, generating cytoskeletal mechanical stimulation and consequently inducing differentiation of mesenchymal stem cells into different lineages. This chapter summarizes the latest development of regulation of mesenchymal stem cell morphology by micro-patterns and the influence on the behaviors and differentiation of the mesenchymal stem cells.

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Acknowledgements

This work was supported by JSPS KAKENHI Grant Number 18K19947, 18K19945 and 19H04475.

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Authors and Affiliations

  1. Research Center for Functional Materials, National Institute for Materials Science, Tsukuba, Ibaraki, Japan

    Guoping Chen & Naoki Kawazoe

Authors
  1. Guoping Chen
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  2. Naoki Kawazoe
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Corresponding author

Correspondence to Guoping Chen .

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Editors and Affiliations

  1. Institute of Cell & Tissue Engineering and College of Medicine, Catholic University of Korea, Seoul, Korea (Republic of)

    Heung Jae Chun

  2. 3B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, Guimarães, Portugal

    Rui L. Reis

  3. Department of Industrial Engineering and BIOtech Research Center, University of Trento, Trento, Italy

    Antonella Motta

  4. Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer BIN Research Center, Jeonbuk National University, Jeonju, Korea (Republic of)

    Gilson Khang

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Chen, G., Kawazoe, N. (2020). Regulation of Stem Cell Functions by Micro-Patterned Structures. In: Chun, H., Reis, R., Motta, A., Khang, G. (eds) Biomimicked Biomaterials. Advances in Experimental Medicine and Biology, vol 1250. Springer, Singapore. https://doi.org/10.1007/978-981-15-3262-7_10

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