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Application of Research Methodologies in Dairy Chemistry

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Dairy Processing: Advanced Research to Applications

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Abstract

Milk is a complex food consisting of numerous constituents, mainly water, fat, protein, lactose, and minerals. All the constituents have different chemical structures and properties. There are various techniques available to identify the constituents both qualitatively and quantitatively. The common techniques used are chromatography, spectroscopy, electrophoresis method, supercritical fluid extraction, immunoassay, and thermal analysis. Chromatography is a separation of molecules between two different phases: mobile phase and stationary phase. It is a fast, accurate, and sensible method. Spectroscopy is the study of the interaction of the light with the material. The movement of a charged molecule in an electric field is electrophoresis. Supercritical fluid extraction is a separation technique used as a preliminary step for the isolation. Antigen and antibody interaction is the basis of immunoassay. Thermal analysis detects the physicochemical change due to exposure of temperature. Chromatography, spectroscopy, and thermal analysis methods can detect the major constituents of milk and milk products, i.e., proteins, fat, vitamins, and lactose. Minerals are generally detected by atomic absorption spectroscopy. Electrophoresis differentiates the milk proteins, and immunoassay is used to detect pesticide, antibiotics, and microbes including pathogens.

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  • 18 August 2020

    The affiliations in the contributors list in the FM for the editors and in the chapters 1, 8, 10, 11 and 14 were swapped incorrectly.

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Author information

Authors and Affiliations

  1. Dairy Chemistry, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India

    Anuradha Kumari

  2. College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India

    Niraj Kumar Singh

  3. National Dairy Development Board Dairy Services, New Delhi, India

    Sonika Choudhary

  4. DST Women Scientist-C, PFC-TIFAC, New Delhi, India

    Dev Priya

  5. Leibniz Institute of Farm Animal Biology (FBN), Dummerstorf, Germany

    Arpna Sharma

Authors
  1. Anuradha Kumari
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  2. Niraj Kumar Singh
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  3. Sonika Choudhary
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  4. Dev Priya
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  5. Arpna Sharma
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Corresponding author

Correspondence to Anuradha Kumari .

Editor information

Editors and Affiliations

  1. Department of Food Science and Technology, Nebraska Innovation Campus (NIC), University of Nebraska, Lincoln, NE, USA

    Jagrani Minj

  2. Department of Dairy Microbiology, College of Dairy Science and Technology, Kerala Veterinary and Animal Sciences University, Thrissur, Kerala, India

    Aparna Sudhakaran V

  3. Dairy Chemistry, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India

    Anuradha Kumari

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Kumari, A., Singh, N.K., Choudhary, S., Priya, D., Sharma, A. (2020). Application of Research Methodologies in Dairy Chemistry. In: Minj, J., Sudhakaran V, A., Kumari, A. (eds) Dairy Processing: Advanced Research to Applications. Springer, Singapore. https://doi.org/10.1007/978-981-15-2608-4_10

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