Abstract
Sesamum indicum L. is an oil crop mentioned in early Hindu legends and shows seeds of different colours. Sesame seeds, oil and other parts of plant are ethno-medicinally significant and have numerous uses from preparations of hair oils to treatments of various ailments in human and cattle. Qualitative and quantitative phytochemical investigations provide the comprehensive account of different types and classes of metabolites and their quantities present in different parts of plant. In preliminary phytochemical investigation in seed, leaf, shoot and root of certified variety of white sesame G1 alkaloids were detected. Quantification of alkaloids present in different parts of G1 variety was performed by non-spectrophotometric (weighing) method and spectrophotometric method. According to both methods, shoots of G1 variety exhibited highest amount of alkaloids. Amount quantified per gram of tissue by weighing method was found to be the lowest in roots whereas the same quantified per millilitre of extract by spectrophotometric method was observed to be the lowest in seeds. Alkaloids present in this plant were further analysed using thin layer chromatography and advanced techniques like liquid chromatography mass spectrophotometry. The former showed the presence of caffeine or compounds of same molecular mass whereas the latter indicated compounds present in proportionately highest amount in all four tissues. The study proposed use of herbage of an agriculture crop as a source of valuable compounds.
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Acknowledgements
I am thankful to Dr. R.S. Zunjarrao, Head and Principal, Department of Botany, Modern College of Arts, Science and Commerce, Shivajinagar, Pune-411005, Maharashtra, India, for all the support to carry out this study.
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Nagpurkar, M., Patil, N.M. (2020). Qualitative and Quantitative Phytochemical Studies in Different Parts of Sesamum indicum L.. In: Khasim, S.M., Long, C., Thammasiri, K., Lutken, H. (eds) Medicinal Plants: Biodiversity, Sustainable Utilization and Conservation. Springer, Singapore. https://doi.org/10.1007/978-981-15-1636-8_35
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